Grapevine virus H

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A288XSD9|A0A288XSD9_9VIRU Coat protein OS=Grapevine virus H OX=2045345 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.01QSKK4 pKa = 8.85EE5 pKa = 3.93VKK7 pKa = 9.48VFKK10 pKa = 10.75VKK12 pKa = 10.5PSTSNFGEE20 pKa = 4.29LNKK23 pKa = 10.05QLSSQKK29 pKa = 10.38VYY31 pKa = 10.91DD32 pKa = 3.73VSSLEE37 pKa = 3.83KK38 pKa = 10.52LFPKK42 pKa = 10.38SIIKK46 pKa = 10.67GNVHH50 pKa = 6.93AEE52 pKa = 3.94IIVKK56 pKa = 9.95NGKK59 pKa = 9.53VDD61 pKa = 3.69VEE63 pKa = 4.37LDD65 pKa = 3.45LLGPDD70 pKa = 3.9GSDD73 pKa = 2.97ALVSYY78 pKa = 8.16GKK80 pKa = 9.72PYY82 pKa = 10.54VHH84 pKa = 6.63VGCIAIALMPHH95 pKa = 6.03GRR97 pKa = 11.84DD98 pKa = 3.48LNGAAHH104 pKa = 6.71LVVRR108 pKa = 11.84DD109 pKa = 3.26GRR111 pKa = 11.84MLSGKK116 pKa = 9.94DD117 pKa = 3.13VLCRR121 pKa = 11.84FQCDD125 pKa = 3.24LKK127 pKa = 11.16KK128 pKa = 10.57RR129 pKa = 11.84LSAFADD135 pKa = 4.13FPNYY139 pKa = 9.38FVSTEE144 pKa = 4.0DD145 pKa = 3.4VLQGFSFHH153 pKa = 7.24LGINADD159 pKa = 3.91DD160 pKa = 4.01LSFRR164 pKa = 11.84DD165 pKa = 3.94GTHH168 pKa = 6.74PFSIEE173 pKa = 3.18ITTILRR179 pKa = 11.84GCDD182 pKa = 2.92EE183 pKa = 4.63SMDD186 pKa = 3.5MRR188 pKa = 11.84YY189 pKa = 10.08ALSTLGSGAYY199 pKa = 8.69QAILNTKK206 pKa = 8.08TDD208 pKa = 3.36GGEE211 pKa = 4.05SQLIKK216 pKa = 10.5YY217 pKa = 8.51QPNDD221 pKa = 3.34VTSVMPEE228 pKa = 3.63VYY230 pKa = 8.83EE231 pKa = 4.32TIKK234 pKa = 10.9KK235 pKa = 10.42LNLGHH240 pKa = 6.55NGKK243 pKa = 8.56LHH245 pKa = 6.86KK246 pKa = 10.18EE247 pKa = 3.97RR248 pKa = 11.84SDD250 pKa = 3.36EE251 pKa = 4.91RR252 pKa = 11.84GDD254 pKa = 3.78KK255 pKa = 10.37DD256 pKa = 5.46APLDD260 pKa = 3.63WGDD263 pKa = 3.61SGG265 pKa = 4.91

Molecular weight: 29.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A288XSC2|A0A288XSC2_9VIRU Uncharacterized protein OS=Grapevine virus H OX=2045345 PE=4 SV=1
MM1 pKa = 7.92EE2 pKa = 6.24DD3 pKa = 3.43PVFLAGRR10 pKa = 11.84SSYY13 pKa = 10.69AKK15 pKa = 9.34KK16 pKa = 10.1RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84ARR21 pKa = 11.84LGICVCGSIMHH32 pKa = 7.13NIDD35 pKa = 3.3NCNKK39 pKa = 9.96SRR41 pKa = 11.84LSSAKK46 pKa = 9.67SDD48 pKa = 3.3RR49 pKa = 11.84LDD51 pKa = 2.98WVKK54 pKa = 10.6YY55 pKa = 9.99GRR57 pKa = 11.84VILEE61 pKa = 4.06DD62 pKa = 3.23EE63 pKa = 4.28TRR65 pKa = 11.84INSAIVRR72 pKa = 11.84RR73 pKa = 11.84VALEE77 pKa = 3.74YY78 pKa = 11.17NLDD81 pKa = 3.66IKK83 pKa = 11.26FEE85 pKa = 4.17GANAPRR91 pKa = 11.84HH92 pKa = 5.66PSRR95 pKa = 11.84SPEE98 pKa = 3.95EE99 pKa = 3.93TPEE102 pKa = 4.09YY103 pKa = 10.57YY104 pKa = 10.25SWW106 pKa = 4.31

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2447

106

1717

489.4

55.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.089 ± 0.784

2.166 ± 0.276

6.866 ± 0.448

6.743 ± 0.596

4.25 ± 0.561

6.62 ± 0.732

2.983 ± 0.415

4.945 ± 0.237

7.356 ± 0.757

11.157 ± 1.13

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.002 ± 0.331

4.046 ± 0.242

3.596 ± 0.27

2.942 ± 0.558

5.394 ± 1.14

7.274 ± 0.637

5.027 ± 0.753

6.048 ± 0.569

0.899 ± 0.246

3.596 ± 0.294
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski