Haliea sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Haliea; unclassified Haliea

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A519IFT1|A0A519IFT1_9GAMM VOC family protein OS=Haliea sp. OX=1932666 GN=EOO54_21010 PE=4 SV=1
MM1 pKa = 8.0ALMCPRR7 pKa = 11.84CGADD11 pKa = 3.04NGDD14 pKa = 3.44TARR17 pKa = 11.84FCKK20 pKa = 10.57SCGEE24 pKa = 4.21GLQAAAPVVQSSGTAPTEE42 pKa = 3.7PSAAPVCSSCGAVNSLGARR61 pKa = 11.84FCKK64 pKa = 10.52SCGTLLPAAASTAAAAAVVTPLPVTSPLQASGAAAFSPASPSLFTEE110 pKa = 5.1PPADD114 pKa = 3.7PVSTALPSPVAADD127 pKa = 3.89TSHH130 pKa = 6.3SPLWILLIAALLVLALGGGGYY151 pKa = 9.68WYY153 pKa = 10.21VQNRR157 pKa = 11.84GMPAFGALMGDD168 pKa = 3.69SDD170 pKa = 4.38PAAGSSDD177 pKa = 3.07AAEE180 pKa = 4.05VEE182 pKa = 4.26KK183 pKa = 11.01QEE185 pKa = 4.89EE186 pKa = 4.67VLTDD190 pKa = 3.26TAEE193 pKa = 4.21AADD196 pKa = 4.01EE197 pKa = 4.09EE198 pKa = 5.3VITEE202 pKa = 4.22VEE204 pKa = 3.62VDD206 pKa = 3.53AAADD210 pKa = 3.76TAADD214 pKa = 3.69AAADD218 pKa = 3.52AAAPAEE224 pKa = 4.57TQASPAA230 pKa = 3.69

Molecular weight:
22.48 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A519IE07|A0A519IE07_9GAMM AAA family ATPase (Fragment) OS=Haliea sp. OX=1932666 GN=EOO54_21355 PE=4 SV=1
MM1 pKa = 7.52NLRR4 pKa = 11.84HH5 pKa = 5.81MEE7 pKa = 3.9VFRR10 pKa = 11.84AVMLTGGVVGAAEE23 pKa = 4.88LLHH26 pKa = 6.64ISQPAVSKK34 pKa = 10.73ILAQAQRR41 pKa = 11.84QLGFALFVRR50 pKa = 11.84IKK52 pKa = 10.81GRR54 pKa = 11.84LVPTAEE60 pKa = 4.15GQALHH65 pKa = 7.01AEE67 pKa = 4.56VEE69 pKa = 4.58TLWRR73 pKa = 11.84SVQRR77 pKa = 11.84VQATPRR83 pKa = 11.84SGTLTLAVSASLAPYY98 pKa = 9.74LVPRR102 pKa = 11.84TLATLARR109 pKa = 11.84RR110 pKa = 11.84FPGLQTRR117 pKa = 11.84MEE119 pKa = 4.17ILVTPIMVDD128 pKa = 3.0ALLDD132 pKa = 3.5RR133 pKa = 11.84TADD136 pKa = 3.05IGVAILPNDD145 pKa = 3.92HH146 pKa = 7.28PNLVQVSRR154 pKa = 11.84HH155 pKa = 5.79DD156 pKa = 3.57CGFACVLPEE165 pKa = 3.8GHH167 pKa = 7.08KK168 pKa = 10.02LAKK171 pKa = 9.78KK172 pKa = 10.45RR173 pKa = 11.84IVTPDD178 pKa = 3.5DD179 pKa = 3.56LVGEE183 pKa = 4.27RR184 pKa = 11.84LIGSPTDD191 pKa = 3.46TPYY194 pKa = 11.43GQALLRR200 pKa = 11.84AYY202 pKa = 9.84GPHH205 pKa = 7.13ASTLNVQSLVRR216 pKa = 11.84SATSACWQAQAGGGIAVVDD235 pKa = 4.66RR236 pKa = 11.84AAVAGTTFRR245 pKa = 11.84QLVVRR250 pKa = 11.84PFQTATRR257 pKa = 11.84LPIAVLHH264 pKa = 5.47NRR266 pKa = 11.84YY267 pKa = 9.4RR268 pKa = 11.84PLSVVQQAFCDD279 pKa = 3.4AFAAVWKK286 pKa = 10.6RR287 pKa = 11.84EE288 pKa = 3.95MPRR291 pKa = 3.2

Molecular weight:
31.59 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5874

0

5874

1496469

24

1916

254.8

27.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.268 ± 0.036

0.949 ± 0.01

5.062 ± 0.026

4.856 ± 0.027

3.572 ± 0.02

8.649 ± 0.031

2.252 ± 0.016

4.234 ± 0.025

3.106 ± 0.027

10.817 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.018

2.563 ± 0.02

5.366 ± 0.028

4.045 ± 0.021

6.637 ± 0.034

5.44 ± 0.025

5.278 ± 0.026

7.693 ± 0.029

1.481 ± 0.014

2.179 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski