Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus subtilis group; Bacillus licheniformis

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4164 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q65EV6|Q65EV6_BACLD Uncharacterized protein OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BL00826 PE=4 SV=1
MM1 pKa = 7.72GYY3 pKa = 10.63DD4 pKa = 3.69PLISALRR11 pKa = 11.84KK12 pKa = 9.67YY13 pKa = 10.61KK14 pKa = 11.06NNDD17 pKa = 4.03LIIEE21 pKa = 4.35WKK23 pKa = 10.53SGLKK27 pKa = 10.58VIGEE31 pKa = 4.34PDD33 pKa = 3.2TLYY36 pKa = 9.94EE37 pKa = 4.21TDD39 pKa = 4.79NGLDD43 pKa = 4.06DD44 pKa = 6.47DD45 pKa = 4.48DD46 pKa = 4.98VNYY49 pKa = 10.32IEE51 pKa = 4.44YY52 pKa = 10.37DD53 pKa = 3.2AVAFQVNNILSQPTNKK69 pKa = 10.25DD70 pKa = 3.03CGVYY74 pKa = 9.97DD75 pKa = 3.84WLRR78 pKa = 11.84QGEE81 pKa = 4.3SSLIEE86 pKa = 3.47ISLYY90 pKa = 10.72EE91 pKa = 4.09DD92 pKa = 3.59TPSAVYY98 pKa = 8.7LTDD101 pKa = 3.69GQKK104 pKa = 9.33IWEE107 pKa = 4.23MDD109 pKa = 3.12SDD111 pKa = 4.03EE112 pKa = 4.19

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65CN8|Q65CN8_BACLD Stage 0 sporulation protein J OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=spo0J PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.27RR12 pKa = 11.84SKK14 pKa = 9.34VHH16 pKa = 5.82GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.55NGRR28 pKa = 11.84LVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4164

0

4164

1201246

22

3588

288.5

32.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.048 ± 0.039

0.768 ± 0.012

5.154 ± 0.033

7.398 ± 0.052

4.551 ± 0.035

7.212 ± 0.045

2.185 ± 0.018

7.155 ± 0.034

7.089 ± 0.048

9.748 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.744 ± 0.019

3.796 ± 0.027

3.751 ± 0.022

3.585 ± 0.025

4.396 ± 0.028

6.028 ± 0.029

5.226 ± 0.034

6.748 ± 0.028

1.03 ± 0.014

3.388 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski