Cucurbit aphid borne yellows virus associated RNA

Taxonomy: Viruses; Riboviria; Virus-associated RNAs

Average proteome isoelectric point is 8.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5BKM2|A0A0C5BKM2_9VIRU RNA-directed RNA polymerase OS=Cucurbit aphid borne yellows virus associated RNA OX=1611875 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 9.39MQLPSFSVGQHH13 pKa = 4.46ITSKK17 pKa = 10.76LVGVMPKK24 pKa = 10.33LGNKK28 pKa = 9.15LATAGKK34 pKa = 10.21VSATCGLVVAAGFSLYY50 pKa = 10.38AYY52 pKa = 10.21RR53 pKa = 11.84KK54 pKa = 8.29VCINKK59 pKa = 10.02LEE61 pKa = 4.09LVRR64 pKa = 11.84INTVPLPDD72 pKa = 4.57DD73 pKa = 4.64CIEE76 pKa = 4.8DD77 pKa = 3.81ALEE80 pKa = 4.38MEE82 pKa = 5.56DD83 pKa = 4.45DD84 pKa = 3.89AASEE88 pKa = 4.03LVQAKK93 pKa = 9.14IAKK96 pKa = 8.27EE97 pKa = 3.73AAIAAALEE105 pKa = 4.18PTKK108 pKa = 10.28PAEE111 pKa = 4.14QFAAAAKK118 pKa = 9.92IRR120 pKa = 11.84AQPPPNPLAKK130 pKa = 10.2RR131 pKa = 11.84MNRR134 pKa = 11.84LRR136 pKa = 11.84PGSTGKK142 pKa = 10.63FIRR145 pKa = 11.84VVRR148 pKa = 11.84AEE150 pKa = 4.4IKK152 pKa = 10.6AQMGTPTITAANEE165 pKa = 3.47AVIRR169 pKa = 11.84HH170 pKa = 5.67MLSKK174 pKa = 10.67YY175 pKa = 9.77CAVRR179 pKa = 11.84NIRR182 pKa = 11.84TSSYY186 pKa = 8.16AHH188 pKa = 5.96LVSRR192 pKa = 11.84VVRR195 pKa = 11.84EE196 pKa = 3.73VMTPYY201 pKa = 10.41PGDD204 pKa = 3.58SEE206 pKa = 4.35QVEE209 pKa = 4.44RR210 pKa = 11.84ASSVVNRR217 pKa = 11.84IHH219 pKa = 6.44NWLVQYY225 pKa = 10.67KK226 pKa = 9.79KK227 pKa = 8.89XGGLEE232 pKa = 3.81LARR235 pKa = 11.84GFTHH239 pKa = 7.0NEE241 pKa = 3.32TRR243 pKa = 11.84TDD245 pKa = 3.64VPGLDD250 pKa = 3.31ATNLGEE256 pKa = 4.81SRR258 pKa = 11.84PLTDD262 pKa = 3.02TNIRR266 pKa = 11.84RR267 pKa = 11.84VVGPVSSDD275 pKa = 3.14YY276 pKa = 11.43DD277 pKa = 3.33IVFYY281 pKa = 10.91TNSRR285 pKa = 11.84NNLMRR290 pKa = 11.84GLVNRR295 pKa = 11.84VLTYY299 pKa = 10.52KK300 pKa = 10.51GGPVLEE306 pKa = 4.38PTPGAWRR313 pKa = 11.84EE314 pKa = 3.99LRR316 pKa = 11.84SLAHH320 pKa = 6.52SLGNRR325 pKa = 11.84CLTTPLSSEE334 pKa = 4.22EE335 pKa = 4.09FLACYY340 pKa = 9.93AGRR343 pKa = 11.84KK344 pKa = 7.38RR345 pKa = 11.84AIYY348 pKa = 10.04AKK350 pKa = 10.25AIEE353 pKa = 4.46SFNTKK358 pKa = 9.49PWDD361 pKa = 3.56ADD363 pKa = 2.67KK364 pKa = 11.83DD365 pKa = 4.06MIVKK369 pKa = 10.42AFIKK373 pKa = 10.43KK374 pKa = 10.09EE375 pKa = 3.78KK376 pKa = 10.62DD377 pKa = 2.99KK378 pKa = 11.24LLSSNSDD385 pKa = 3.05PRR387 pKa = 11.84IIQPRR392 pKa = 11.84HH393 pKa = 4.93PRR395 pKa = 11.84FLVSLGRR402 pKa = 11.84YY403 pKa = 8.4IRR405 pKa = 11.84PLEE408 pKa = 3.8QKK410 pKa = 10.11VYY412 pKa = 9.13KK413 pKa = 10.2TYY415 pKa = 10.28TRR417 pKa = 11.84LFKK420 pKa = 10.79RR421 pKa = 11.84FAATNTPVCFKK432 pKa = 10.51GLNYY436 pKa = 9.99LRR438 pKa = 11.84RR439 pKa = 11.84GSCLKK444 pKa = 10.55EE445 pKa = 3.51KK446 pKa = 10.09WEE448 pKa = 4.43SFNSPVAVCLDD459 pKa = 3.62ASRR462 pKa = 11.84FDD464 pKa = 3.57LHH466 pKa = 8.19VSEE469 pKa = 6.09DD470 pKa = 4.03ALQHH474 pKa = 4.57THH476 pKa = 6.44LLYY479 pKa = 9.86RR480 pKa = 11.84TAIGNDD486 pKa = 3.4PLLAYY491 pKa = 9.57ILEE494 pKa = 4.39NRR496 pKa = 11.84LVTDD500 pKa = 4.44GVGWSSDD507 pKa = 2.88GAYY510 pKa = 9.8RR511 pKa = 11.84YY512 pKa = 9.02HH513 pKa = 7.04KK514 pKa = 10.61RR515 pKa = 11.84GGRR518 pKa = 11.84CSGDD522 pKa = 3.04NDD524 pKa = 3.61TSLGNVIIMLSITHH538 pKa = 6.95AFCEE542 pKa = 4.37EE543 pKa = 4.14SEE545 pKa = 4.21IPHH548 pKa = 7.15IEE550 pKa = 4.07VANDD554 pKa = 3.32GDD556 pKa = 3.9DD557 pKa = 3.24QVIIVEE563 pKa = 4.37SEE565 pKa = 3.95HH566 pKa = 6.94VEE568 pKa = 3.82KK569 pKa = 10.87VLRR572 pKa = 11.84IEE574 pKa = 5.12DD575 pKa = 3.37IFRR578 pKa = 11.84EE579 pKa = 4.24FGFLLKK585 pKa = 10.6VEE587 pKa = 4.53EE588 pKa = 4.52PVHH591 pKa = 5.09EE592 pKa = 4.32LEE594 pKa = 6.34RR595 pKa = 11.84IDD597 pKa = 4.2FCQTRR602 pKa = 11.84PVQLTPDD609 pKa = 3.24VCTMVRR615 pKa = 11.84HH616 pKa = 6.23PRR618 pKa = 11.84LAMTKK623 pKa = 10.27DD624 pKa = 3.25LTTFIPIEE632 pKa = 4.15RR633 pKa = 11.84GKK635 pKa = 10.76LKK637 pKa = 10.85YY638 pKa = 10.34HH639 pKa = 6.45MLTAIGKK646 pKa = 9.8CGMACYY652 pKa = 10.66NDD654 pKa = 3.76IPVLGAMYY662 pKa = 10.26RR663 pKa = 11.84KK664 pKa = 9.18LVKK667 pKa = 9.92IGSMNKK673 pKa = 9.81GNEE676 pKa = 3.85EE677 pKa = 4.01RR678 pKa = 11.84WWKK681 pKa = 8.27STHH684 pKa = 6.77ADD686 pKa = 3.3PAFRR690 pKa = 11.84SLSSKK695 pKa = 10.51AATNVGLTPQCRR707 pKa = 11.84ASFWKK712 pKa = 10.72AFGILPDD719 pKa = 3.57QQVALEE725 pKa = 4.51KK726 pKa = 10.32EE727 pKa = 4.31WNDD730 pKa = 3.53WEE732 pKa = 4.24PLLDD736 pKa = 4.61KK737 pKa = 10.78IEE739 pKa = 4.26YY740 pKa = 8.68VHH742 pKa = 6.81SFLGDD747 pKa = 3.54YY748 pKa = 7.89PVCEE752 pKa = 4.23GDD754 pKa = 3.51VV755 pKa = 3.25

Molecular weight: 84.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5BKM2|A0A0C5BKM2_9VIRU RNA-directed RNA polymerase OS=Cucurbit aphid borne yellows virus associated RNA OX=1611875 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 9.39MQLPSFSVGQHH13 pKa = 4.46ITSKK17 pKa = 10.76LVGVMPKK24 pKa = 10.33LGNKK28 pKa = 9.15LATAGKK34 pKa = 10.21VSATCGLVVAAGFSLYY50 pKa = 10.38AYY52 pKa = 10.21RR53 pKa = 11.84KK54 pKa = 8.29VCINKK59 pKa = 10.02LEE61 pKa = 4.09LVRR64 pKa = 11.84INTVPLPDD72 pKa = 4.57DD73 pKa = 4.64CIEE76 pKa = 4.8DD77 pKa = 3.81ALEE80 pKa = 4.38MEE82 pKa = 5.56DD83 pKa = 4.45DD84 pKa = 3.89AASEE88 pKa = 4.03LVQAKK93 pKa = 9.14IAKK96 pKa = 8.27EE97 pKa = 3.73AAIAAALEE105 pKa = 4.18PTKK108 pKa = 10.28PAEE111 pKa = 4.14QFAAAAKK118 pKa = 9.92IRR120 pKa = 11.84AQPPPNPLAKK130 pKa = 10.2RR131 pKa = 11.84MNRR134 pKa = 11.84LRR136 pKa = 11.84PGSTGKK142 pKa = 10.63FIRR145 pKa = 11.84VVRR148 pKa = 11.84AEE150 pKa = 4.4IKK152 pKa = 10.6AQMGTPTITAANEE165 pKa = 3.47AVIRR169 pKa = 11.84HH170 pKa = 5.67MLSKK174 pKa = 10.67YY175 pKa = 9.77CAVRR179 pKa = 11.84NIRR182 pKa = 11.84TSSYY186 pKa = 8.16AHH188 pKa = 5.96LVSRR192 pKa = 11.84VVRR195 pKa = 11.84EE196 pKa = 3.73VMTPYY201 pKa = 10.41PGDD204 pKa = 3.58SEE206 pKa = 4.35QVEE209 pKa = 4.44RR210 pKa = 11.84ASSVVNRR217 pKa = 11.84IHH219 pKa = 6.44NWLVQYY225 pKa = 10.87KK226 pKa = 9.86KK227 pKa = 10.91

Molecular weight: 24.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

982

227

755

491.0

54.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.572 ± 1.908

2.138 ± 0.176

4.481 ± 0.858

6.212 ± 0.227

2.953 ± 0.556

5.601 ± 0.558

2.342 ± 0.271

5.499 ± 0.106

6.619 ± 0.406

8.859 ± 0.64

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.546 ± 0.457

4.175 ± 0.098

5.601 ± 0.265

2.546 ± 0.457

7.026 ± 0.195

6.11 ± 0.027

5.397 ± 0.258

8.045 ± 0.769

1.12 ± 0.317

3.055 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski