Hartmannibacter diazotrophicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Pleomorphomonadaceae; Hartmannibacter

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C9D3S9|A0A2C9D3S9_9RHIZ Bacteriophage head-tail adaptor OS=Hartmannibacter diazotrophicus OX=1482074 GN=HDIA_1342 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.27LKK4 pKa = 10.76SILLGTAALAGMTHH18 pKa = 6.81GALAADD24 pKa = 4.64AMAEE28 pKa = 4.17APVPTAVNYY37 pKa = 10.4VEE39 pKa = 5.18VCDD42 pKa = 3.93AQGAGFFVIPGKK54 pKa = 7.46EE55 pKa = 3.95TCFKK59 pKa = 10.13IDD61 pKa = 2.98GRR63 pKa = 11.84VRR65 pKa = 11.84TGFKK69 pKa = 10.19YY70 pKa = 10.88DD71 pKa = 3.56EE72 pKa = 5.69DD73 pKa = 4.22GTTNPAGGTADD84 pKa = 3.48AYY86 pKa = 9.94TWFADD91 pKa = 3.29ARR93 pKa = 11.84IGFDD97 pKa = 3.2ARR99 pKa = 11.84TASDD103 pKa = 3.69YY104 pKa = 11.49GPVRR108 pKa = 11.84SYY110 pKa = 11.24FRR112 pKa = 11.84IKK114 pKa = 10.45VSSSGNVVADD124 pKa = 3.46QAFVQVGYY132 pKa = 10.96LLTGYY137 pKa = 10.4SDD139 pKa = 4.03SDD141 pKa = 3.62VLAAAGLDD149 pKa = 3.52EE150 pKa = 4.43TGEE153 pKa = 4.11YY154 pKa = 11.18GNFRR158 pKa = 11.84DD159 pKa = 3.98VTFGLPTDD167 pKa = 4.6DD168 pKa = 5.19NGGLQFDD175 pKa = 5.25LLADD179 pKa = 4.04DD180 pKa = 4.86LGGGFFAGVQVIDD193 pKa = 4.08GGAAGTFSQQYY204 pKa = 9.09NKK206 pKa = 9.29TAEE209 pKa = 4.11SVAFGGVVGITGQPWGAASLFAMYY233 pKa = 10.25DD234 pKa = 3.56DD235 pKa = 5.38EE236 pKa = 7.31DD237 pKa = 4.36DD238 pKa = 3.96QVSVRR243 pKa = 11.84ATGNFNVMEE252 pKa = 4.61GLQIAAVIGYY262 pKa = 6.94MTNNDD267 pKa = 3.41GNVGYY272 pKa = 8.44MAQGNVDD279 pKa = 3.86TVALGLGASYY289 pKa = 11.35DD290 pKa = 3.57VTSDD294 pKa = 3.41VNVYY298 pKa = 10.64LSGIYY303 pKa = 10.3GFNDD307 pKa = 2.82NSTSDD312 pKa = 3.45SYY314 pKa = 11.89DD315 pKa = 3.29ILGGAAWTFAEE326 pKa = 4.84GTSLVGEE333 pKa = 4.36VSYY336 pKa = 11.16TDD338 pKa = 3.37NPGVSGAVGATVGLIRR354 pKa = 11.84KK355 pKa = 6.94WW356 pKa = 3.18

Molecular weight: 36.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C9D1C2|A0A2C9D1C2_9RHIZ Acetoacetyl CoA synthase NphT7 OS=Hartmannibacter diazotrophicus OX=1482074 GN=nphT7 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4966

0

4966

1563237

29

2507

314.8

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.354 ± 0.043

0.847 ± 0.012

5.963 ± 0.03

5.835 ± 0.032

3.812 ± 0.021

8.604 ± 0.029

2.012 ± 0.018

5.364 ± 0.023

3.326 ± 0.027

10.098 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.589 ± 0.017

2.494 ± 0.017

5.122 ± 0.025

2.801 ± 0.018

6.846 ± 0.033

5.58 ± 0.029

5.366 ± 0.024

7.594 ± 0.028

1.246 ± 0.012

2.148 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski