Pseudomonas guineae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3M7K8|A0A1I3M7K8_9PSED Protease I OS=Pseudomonas guineae OX=425504 GN=SAMN05216206_3190 PE=3 SV=1
MM1 pKa = 7.66PPIDD5 pKa = 4.4TEE7 pKa = 4.09QQLAARR13 pKa = 11.84LAILEE18 pKa = 4.13NPEE21 pKa = 4.11TQDD24 pKa = 3.31CTVYY28 pKa = 10.7RR29 pKa = 11.84ADD31 pKa = 3.75EE32 pKa = 3.97QDD34 pKa = 3.65PEE36 pKa = 4.29AEE38 pKa = 4.18EE39 pKa = 4.63VDD41 pKa = 4.03LGDD44 pKa = 5.2AKK46 pKa = 11.32VLFCGPFEE54 pKa = 5.7APADD58 pKa = 3.35WDD60 pKa = 3.51AAEE63 pKa = 4.15RR64 pKa = 11.84EE65 pKa = 4.53EE66 pKa = 4.57YY67 pKa = 10.56FGDD70 pKa = 3.7SAPEE74 pKa = 3.8LFVNARR80 pKa = 11.84IEE82 pKa = 4.37CEE84 pKa = 3.84AKK86 pKa = 10.27PGSKK90 pKa = 9.6SHH92 pKa = 6.48FSVDD96 pKa = 2.61IGDD99 pKa = 3.96YY100 pKa = 10.46VAAQPGEE107 pKa = 4.31GEE109 pKa = 4.12VVMFYY114 pKa = 10.97VHH116 pKa = 7.86DD117 pKa = 4.12YY118 pKa = 11.61LEE120 pKa = 5.76DD121 pKa = 3.85DD122 pKa = 3.74TGCTYY127 pKa = 11.26VLLRR131 pKa = 11.84DD132 pKa = 3.99EE133 pKa = 4.95EE134 pKa = 4.39VLEE137 pKa = 4.26

Molecular weight: 15.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3FLK6|A0A1I3FLK6_9PSED ATP synthase epsilon chain OS=Pseudomonas guineae OX=425504 GN=atpC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.06SGRR28 pKa = 11.84AVLQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3794

0

3794

1233122

29

3029

325.0

35.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.176 ± 0.04

1.031 ± 0.014

5.226 ± 0.032

5.854 ± 0.036

3.611 ± 0.026

7.78 ± 0.04

2.248 ± 0.019

4.857 ± 0.027

3.506 ± 0.032

12.233 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.018

3.061 ± 0.025

4.669 ± 0.024

4.914 ± 0.038

6.223 ± 0.038

5.898 ± 0.026

4.573 ± 0.027

6.913 ± 0.039

1.402 ± 0.017

2.479 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski