Mycoplasma putrefaciens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1378 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B0Q606|A0A3B0Q606_9MOLU Predicted metal-dependent RNase consists of a metallo-beta-lactamase domain and an RNA-binding KH domain (Fragment) OS=Mycoplasma putrefaciens OX=2123 GN=NCTC10155_00827 PE=4 SV=1
MM1 pKa = 8.1IFDD4 pKa = 4.14QPLKK8 pKa = 10.69AITPGQQAVFYY19 pKa = 10.48LDD21 pKa = 4.52EE22 pKa = 5.09ICLGGAVIDD31 pKa = 4.12QVNN34 pKa = 2.84

Molecular weight: 3.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B0PKL4|A0A3B0PKL4_9MOLU Uncharacterized protein (Fragment) OS=Mycoplasma putrefaciens OX=2123 GN=NCTC10155_00316 PE=4 SV=1
MM1 pKa = 7.49TNNKK5 pKa = 9.42VIYY8 pKa = 9.86RR9 pKa = 11.84GTGRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 7.99TSIAQVILTPGSGKK29 pKa = 10.82VIVNGKK35 pKa = 9.4AALDD39 pKa = 3.71FFPYY43 pKa = 9.16ATLVQDD49 pKa = 4.87LEE51 pKa = 4.4QPLLATGTLKK61 pKa = 11.13DD62 pKa = 3.47FDD64 pKa = 3.59ITVKK68 pKa = 10.78VNGGGFTGQAGATRR82 pKa = 11.84LGIARR87 pKa = 11.84ALLQASEE94 pKa = 5.01DD95 pKa = 3.58YY96 pKa = 10.84RR97 pKa = 11.84KK98 pKa = 10.24ALRR101 pKa = 11.84NEE103 pKa = 4.02GLLTRR108 pKa = 11.84DD109 pKa = 3.2ARR111 pKa = 11.84IKK113 pKa = 9.97EE114 pKa = 4.0RR115 pKa = 11.84KK116 pKa = 9.54KK117 pKa = 10.2YY118 pKa = 9.78GLRR121 pKa = 11.84GARR124 pKa = 11.84RR125 pKa = 11.84APQFSKK131 pKa = 10.95RR132 pKa = 3.57

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1378

0

1378

173983

29

747

126.3

14.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.596 ± 0.096

0.604 ± 0.021

5.741 ± 0.067

5.948 ± 0.083

4.945 ± 0.06

4.694 ± 0.079

1.383 ± 0.034

9.614 ± 0.08

9.958 ± 0.085

9.951 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.036

7.056 ± 0.083

2.699 ± 0.079

4.429 ± 0.059

2.902 ± 0.057

6.457 ± 0.076

5.42 ± 0.061

5.961 ± 0.064

0.633 ± 0.018

3.682 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski