Wissadula yellow mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8GVB5|A0A1D8GVB5_9GEMI Capsid protein OS=Wissadula yellow mosaic virus OX=1904884 GN=CP PE=3 SV=1
MM1 pKa = 7.39PRR3 pKa = 11.84KK4 pKa = 9.22PSSFRR9 pKa = 11.84LSAKK13 pKa = 10.46NIFLTYY19 pKa = 8.62PQCDD23 pKa = 3.5LSKK26 pKa = 11.49DD27 pKa = 3.33EE28 pKa = 4.72ALQMLHH34 pKa = 5.99ALKK37 pKa = 10.21WSVVKK42 pKa = 8.52PTYY45 pKa = 10.14IRR47 pKa = 11.84VARR50 pKa = 11.84EE51 pKa = 3.67EE52 pKa = 4.28HH53 pKa = 7.09SDD55 pKa = 3.73GLPHH59 pKa = 6.51LHH61 pKa = 7.07CLIQLSGKK69 pKa = 10.24SNIKK73 pKa = 9.95DD74 pKa = 3.22VRR76 pKa = 11.84FFDD79 pKa = 3.57LTHH82 pKa = 6.69PRR84 pKa = 11.84RR85 pKa = 11.84SASFHH90 pKa = 6.84PNVQAAKK97 pKa = 9.12DD98 pKa = 3.63TNAVKK103 pKa = 10.63NYY105 pKa = 6.66ITKK108 pKa = 10.56EE109 pKa = 3.5GDD111 pKa = 3.42YY112 pKa = 10.73CEE114 pKa = 4.54SGQYY118 pKa = 8.42TVSGGTRR125 pKa = 11.84SNKK128 pKa = 10.04DD129 pKa = 3.42DD130 pKa = 3.7VFHH133 pKa = 6.76NAVNAPSVGEE143 pKa = 3.67ALEE146 pKa = 4.46IIKK149 pKa = 10.91AGDD152 pKa = 3.42PKK154 pKa = 10.67TFLVNYY160 pKa = 9.81HH161 pKa = 5.54NLKK164 pKa = 10.84GNLEE168 pKa = 4.05RR169 pKa = 11.84LFAKK173 pKa = 10.39APEE176 pKa = 4.09PWVPPFQLSSFTNVPDD192 pKa = 5.1EE193 pKa = 4.13MQEE196 pKa = 3.8WADD199 pKa = 3.73EE200 pKa = 4.25YY201 pKa = 11.01FGRR204 pKa = 11.84GSAARR209 pKa = 11.84PEE211 pKa = 4.12RR212 pKa = 11.84PISIIVEE219 pKa = 4.05GDD221 pKa = 3.08SRR223 pKa = 11.84TGKK226 pKa = 8.52TMWARR231 pKa = 11.84SLGPHH236 pKa = 6.57NYY238 pKa = 10.17LSGHH242 pKa = 6.86LDD244 pKa = 3.63FNARR248 pKa = 11.84IYY250 pKa = 10.96SNEE253 pKa = 3.69VEE255 pKa = 4.57FNVIDD260 pKa = 4.25DD261 pKa = 4.32VSPQYY266 pKa = 11.29LKK268 pKa = 10.82LKK270 pKa = 9.19HH271 pKa = 5.51WKK273 pKa = 9.67EE274 pKa = 3.98LIGAQKK280 pKa = 10.57DD281 pKa = 3.42WQSNCKK287 pKa = 9.38YY288 pKa = 10.21GKK290 pKa = 9.18PVQIKK295 pKa = 10.76GGMDD299 pKa = 3.62PSNRR303 pKa = 11.84ALQSWSGISYY313 pKa = 11.01KK314 pKa = 10.92DD315 pKa = 3.66FLDD318 pKa = 4.07KK319 pKa = 11.41EE320 pKa = 4.73EE321 pKa = 4.14NTSLRR326 pKa = 11.84NWTIKK331 pKa = 10.32NAVFITLDD339 pKa = 3.31SPLYY343 pKa = 10.31QEE345 pKa = 4.5STQAGQEE352 pKa = 4.07EE353 pKa = 5.23GHH355 pKa = 6.1QEE357 pKa = 3.92TANN360 pKa = 3.42

Molecular weight: 40.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8GVC6|A0A1D8GVC6_9GEMI Replication enhancer OS=Wissadula yellow mosaic virus OX=1904884 GN=REn PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.61RR4 pKa = 11.84DD5 pKa = 3.78APWRR9 pKa = 11.84SMAGTSKK16 pKa = 10.56VSRR19 pKa = 11.84NANYY23 pKa = 10.24SPRR26 pKa = 11.84GGYY29 pKa = 8.07GLKK32 pKa = 10.33FNKK35 pKa = 9.46AAEE38 pKa = 4.24WVNRR42 pKa = 11.84PMYY45 pKa = 9.79RR46 pKa = 11.84KK47 pKa = 8.95PRR49 pKa = 11.84IYY51 pKa = 9.6RR52 pKa = 11.84TLRR55 pKa = 11.84TPDD58 pKa = 3.21VPRR61 pKa = 11.84GCEE64 pKa = 4.4GPCKK68 pKa = 9.6IQSYY72 pKa = 7.68DD73 pKa = 3.35QRR75 pKa = 11.84HH76 pKa = 6.66DD77 pKa = 3.18IAHH80 pKa = 6.49TGKK83 pKa = 10.46VMCISDD89 pKa = 3.3VSRR92 pKa = 11.84GNGIAQRR99 pKa = 11.84VGKK102 pKa = 9.34RR103 pKa = 11.84FCVKK107 pKa = 9.73SVYY110 pKa = 10.25IIGKK114 pKa = 8.8VWMDD118 pKa = 4.5DD119 pKa = 3.6NIKK122 pKa = 10.64LKK124 pKa = 10.74NHH126 pKa = 5.99TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.75RR138 pKa = 11.84RR139 pKa = 11.84PNGNAMDD146 pKa = 4.77FGHH149 pKa = 7.56LFNMYY154 pKa = 10.64DD155 pKa = 4.01NEE157 pKa = 4.36PSTATVKK164 pKa = 10.85NDD166 pKa = 2.82LRR168 pKa = 11.84DD169 pKa = 3.52RR170 pKa = 11.84FQVLHH175 pKa = 6.7RR176 pKa = 11.84FNAKK180 pKa = 8.04VTGGQYY186 pKa = 11.1ASNEE190 pKa = 3.84QALVRR195 pKa = 11.84RR196 pKa = 11.84FWKK199 pKa = 10.46VNNHH203 pKa = 3.68VTYY206 pKa = 10.85NNQEE210 pKa = 3.68AARR213 pKa = 11.84YY214 pKa = 7.48EE215 pKa = 4.13NHH217 pKa = 6.5TEE219 pKa = 3.94NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.66FYY246 pKa = 11.34DD247 pKa = 3.3SVLNN251 pKa = 4.02

Molecular weight: 29.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1003

129

360

200.6

23.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.484 ± 0.927

1.695 ± 0.588

4.586 ± 0.673

4.686 ± 0.765

4.088 ± 0.245

5.184 ± 0.64

3.689 ± 0.56

5.085 ± 0.494

5.882 ± 0.766

7.178 ± 0.738

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.193 ± 0.604

6.68 ± 0.756

5.384 ± 0.604

4.586 ± 0.956

6.879 ± 1.001

8.275 ± 1.325

6.281 ± 1.208

6.082 ± 0.898

1.894 ± 0.168

4.187 ± 0.714
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski