Geodermatophilus sp. DF01_2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Geodermatophilus; unclassified Geodermatophilus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4069 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9N948|A0A4Y9N948_9ACTN Phosphoenolpyruvate carboxykinase [GTP] OS=Geodermatophilus sp. DF01_2 OX=2559610 GN=pckG PE=3 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84RR3 pKa = 11.84AAVALLATAALLLVSGCSVVVVGRR27 pKa = 11.84PSAAQPPAADD37 pKa = 3.66VPPGEE42 pKa = 4.4VGVAGATEE50 pKa = 4.07EE51 pKa = 4.26PVDD54 pKa = 3.78VLARR58 pKa = 11.84NALADD63 pKa = 3.97LEE65 pKa = 4.92TYY67 pKa = 7.49WTEE70 pKa = 3.62QFPDD74 pKa = 3.27VFGQEE79 pKa = 4.18FVPLQGGYY87 pKa = 10.57FSVDD91 pKa = 3.36PDD93 pKa = 4.09DD94 pKa = 4.83VDD96 pKa = 3.67PAAYY100 pKa = 8.43PQGIGCGEE108 pKa = 4.4DD109 pKa = 3.48PRR111 pKa = 11.84DD112 pKa = 3.59VEE114 pKa = 5.52SNAFYY119 pKa = 10.67CQAPDD124 pKa = 3.76APNSDD129 pKa = 3.97SISYY133 pKa = 10.51DD134 pKa = 3.0RR135 pKa = 11.84AFLQEE140 pKa = 3.95LAEE143 pKa = 4.1QYY145 pKa = 11.33GRR147 pKa = 11.84FIPALVMAHH156 pKa = 6.2EE157 pKa = 5.39FGHH160 pKa = 6.49AVQGRR165 pKa = 11.84VGYY168 pKa = 9.0PDD170 pKa = 5.09DD171 pKa = 5.1SISVEE176 pKa = 4.11TQADD180 pKa = 4.0CFAGAWTAWVADD192 pKa = 3.98GQAEE196 pKa = 4.23HH197 pKa = 6.15SQIRR201 pKa = 11.84APEE204 pKa = 4.1LDD206 pKa = 3.48EE207 pKa = 4.08VLRR210 pKa = 11.84GYY212 pKa = 11.13LLLRR216 pKa = 11.84DD217 pKa = 3.88PVGVTTEE224 pKa = 4.11AAHH227 pKa = 6.46GSYY230 pKa = 10.46FDD232 pKa = 3.78RR233 pKa = 11.84VSAFQEE239 pKa = 4.48GFDD242 pKa = 4.93AGPTVCRR249 pKa = 11.84DD250 pKa = 2.93EE251 pKa = 4.6FTAQRR256 pKa = 11.84PYY258 pKa = 10.16TQDD261 pKa = 2.83AFSTANEE268 pKa = 4.01AASGGNAPFGLAQQYY283 pKa = 9.99AAEE286 pKa = 4.56ALPEE290 pKa = 3.91FWDD293 pKa = 3.64RR294 pKa = 11.84AFAAEE299 pKa = 4.09FGEE302 pKa = 4.7TFVSPTLEE310 pKa = 4.31PFAGTAPTCAPDD322 pKa = 4.7DD323 pKa = 4.23RR324 pKa = 11.84DD325 pKa = 3.98LVLCADD331 pKa = 4.5EE332 pKa = 5.77DD333 pKa = 4.24LVGYY337 pKa = 8.37DD338 pKa = 3.26VRR340 pKa = 11.84DD341 pKa = 3.59LVGPAYY347 pKa = 9.58EE348 pKa = 4.1QLEE351 pKa = 4.43SADD354 pKa = 3.72YY355 pKa = 11.12AVITAASLPYY365 pKa = 10.54ALAARR370 pKa = 11.84EE371 pKa = 3.87QLGLSTDD378 pKa = 3.6DD379 pKa = 3.33EE380 pKa = 4.42AAIRR384 pKa = 11.84SAVCLTGWFSRR395 pKa = 11.84AFFDD399 pKa = 5.53GEE401 pKa = 4.42LEE403 pKa = 4.2AADD406 pKa = 4.51ISPGDD411 pKa = 3.39IDD413 pKa = 4.02EE414 pKa = 4.95AVQFLLTYY422 pKa = 8.74GTDD425 pKa = 3.37PSVVPEE431 pKa = 4.06VEE433 pKa = 3.88LTGFQLVDD441 pKa = 3.55LFRR444 pKa = 11.84NGFFDD449 pKa = 4.68GAAACDD455 pKa = 3.55VGLL458 pKa = 4.5

Molecular weight: 48.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9MHB7|A0A4Y9MHB7_9ACTN GAF domain-containing protein OS=Geodermatophilus sp. DF01_2 OX=2559610 GN=E4P41_18625 PE=4 SV=1
RR1 pKa = 7.57PGAPGAAVRR10 pKa = 11.84RR11 pKa = 11.84PPLAPRR17 pKa = 11.84PAAGRR22 pKa = 11.84PRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84PRR29 pKa = 11.84SAGRR33 pKa = 11.84PRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84PAAGSRR43 pKa = 11.84RR44 pKa = 11.84PARR47 pKa = 11.84RR48 pKa = 11.84SAPARR53 pKa = 11.84ARR55 pKa = 11.84RR56 pKa = 11.84ASRR59 pKa = 11.84PAAGGRR65 pKa = 11.84APAPPSRR72 pKa = 11.84CRR74 pKa = 11.84SGG76 pKa = 3.28

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4069

0

4069

1250318

32

2622

307.3

32.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.307 ± 0.062

0.721 ± 0.009

6.25 ± 0.03

5.781 ± 0.039

2.487 ± 0.021

9.66 ± 0.038

2.048 ± 0.018

2.673 ± 0.027

1.311 ± 0.021

10.628 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.588 ± 0.014

1.393 ± 0.016

6.329 ± 0.031

2.697 ± 0.019

8.542 ± 0.042

4.688 ± 0.027

5.839 ± 0.026

9.87 ± 0.036

1.444 ± 0.017

1.744 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski