Proteome-pI 2.0 - Proteome Isoelectric Point Database

Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; Shewanella pealeana

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 4181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A8H240|A8H240_SHEPA 5'-nucleotidase OS=Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) OX=398579 GN=Spea_1300 PE=3 SV=1

MM1 pKa = 7.47NNQNEE6 pKa = 4.25KK7 pKa = 10.79SINNHH12 pKa = 6.15LGLLFIMTALLSACGGGGDD31 pKa = 4.89GEE33 pKa = 5.07SDD35 pKa = 3.61TPAPIATPTEE45 pKa = 4.54TTTSTDD51 pKa = 2.84TTTTEE56 pKa = 5.17DD57 pKa = 3.81PTPDD61 pKa = 3.55PAADD65 pKa = 3.69STSLDD70 pKa = 3.98DD71 pKa = 5.51LVVDD75 pKa = 5.03ADD77 pKa = 3.98NKK79 pKa = 11.05LEE81 pKa = 4.07SAYY84 pKa = 11.1LLTIDD89 pKa = 3.65VDD91 pKa = 4.0RR92 pKa = 11.84VSTEE96 pKa = 3.2RR97 pKa = 11.84GYY99 pKa = 11.36FSLCDD104 pKa = 3.46NYY106 pKa = 10.34QAQGDD111 pKa = 4.78GYY113 pKa = 10.79SVNFDD118 pKa = 3.22SCLFRR123 pKa = 11.84GPLTDD128 pKa = 3.41GQLNKK133 pKa = 9.87EE134 pKa = 4.35VKK136 pKa = 9.43VANHH140 pKa = 6.37EE141 pKa = 4.3SQLIAVIWFYY151 pKa = 11.16EE152 pKa = 4.33GQAPQFVEE160 pKa = 3.87WQYY163 pKa = 11.72SQDD166 pKa = 3.22SDD168 pKa = 4.14TQVLTMNN175 pKa = 4.73

Molecular weight: 19.05 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A8GYG4|A8GYG4_SHEPA Protoporphyrinogen IX dehydrogenase [quinone] OS=Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) OX=398579 GN=hemG PE=3 SV=1

MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVGGRR29 pKa = 11.84KK30 pKa = 9.3VIARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.35GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
4181	0	4181	1420895	31	5444	339.8	37.58

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.069 ± 0.038	1.086 ± 0.016	5.571 ± 0.042	6.084 ± 0.033	4.045 ± 0.025	6.885 ± 0.04	2.119 ± 0.02	6.354 ± 0.031	5.196 ± 0.036	10.576 ± 0.052

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.647 ± 0.023	4.258 ± 0.028	3.829 ± 0.025	4.69 ± 0.034	4.249 ± 0.034	6.945 ± 0.034	5.309 ± 0.036	6.842 ± 0.031	1.197 ± 0.016	3.049 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski