Alfalfa leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Capulavirus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0PRX7|A0A0K0PRX7_9GEMI Replication-associated protein OS=Alfalfa leaf curl virus OX=1306546 GN=repA PE=3 SV=1
MM1 pKa = 7.78EE2 pKa = 4.83SRR4 pKa = 11.84LYY6 pKa = 10.93LLEE9 pKa = 4.68EE10 pKa = 4.33LPATYY15 pKa = 10.43GKK17 pKa = 10.4LQGFCTDD24 pKa = 4.34LFLAEE29 pKa = 4.5QIRR32 pKa = 11.84KK33 pKa = 9.71ASEE36 pKa = 3.38LKK38 pKa = 9.71MFKK41 pKa = 9.65EE42 pKa = 3.96AQMYY46 pKa = 8.0MVLRR50 pKa = 11.84QAFRR54 pKa = 11.84RR55 pKa = 11.84SQRR58 pKa = 11.84NKK60 pKa = 10.29APWPSKK66 pKa = 9.3VAQFNMDD73 pKa = 3.73LALTISRR80 pKa = 11.84AKK82 pKa = 10.29QISEE86 pKa = 4.01EE87 pKa = 4.0AQLLVDD93 pKa = 3.72YY94 pKa = 10.28KK95 pKa = 11.18KK96 pKa = 10.81KK97 pKa = 10.56IEE99 pKa = 4.92DD100 pKa = 3.59GSHH103 pKa = 6.91EE104 pKa = 3.74IRR106 pKa = 11.84EE107 pKa = 4.36NVWIGPGPFLGSEE120 pKa = 4.01EE121 pKa = 4.58GEE123 pKa = 4.09NDD125 pKa = 3.37SSLSTNTTWADD136 pKa = 3.23SEE138 pKa = 4.54AQLSSEE144 pKa = 5.22DD145 pKa = 4.81PICSSS150 pKa = 3.58

Molecular weight: 17.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0PS93|A0A0K0PS93_9GEMI V4 OS=Alfalfa leaf curl virus OX=1306546 GN=V4 PE=4 SV=1
MM1 pKa = 6.99VRR3 pKa = 11.84TRR5 pKa = 11.84SGRR8 pKa = 11.84TYY10 pKa = 11.18GSAQALSWGRR20 pKa = 11.84KK21 pKa = 5.75RR22 pKa = 11.84ARR24 pKa = 11.84TTVRR28 pKa = 11.84SRR30 pKa = 11.84PTLLGPIRR38 pKa = 11.84RR39 pKa = 11.84PSYY42 pKa = 9.03QVKK45 pKa = 8.56TRR47 pKa = 11.84YY48 pKa = 9.57APHH51 pKa = 6.95RR52 pKa = 11.84PQTKK56 pKa = 8.87IHH58 pKa = 6.17SLANTRR64 pKa = 11.84VVSGANEE71 pKa = 4.52GYY73 pKa = 8.33GWHH76 pKa = 6.24VSGVPIGSGFEE87 pKa = 4.22DD88 pKa = 3.12RR89 pKa = 11.84HH90 pKa = 6.45SDD92 pKa = 3.52KK93 pKa = 11.16IKK95 pKa = 10.7INSLNFKK102 pKa = 8.3MQMMTSDD109 pKa = 4.43AGTQTTLWHH118 pKa = 5.61NVYY121 pKa = 9.92MFLVKK126 pKa = 10.56DD127 pKa = 3.82NSGGAQVPKK136 pKa = 10.4FNSICMMDD144 pKa = 3.73NSNPATAEE152 pKa = 3.64IDD154 pKa = 3.47HH155 pKa = 6.98DD156 pKa = 4.41SKK158 pKa = 11.8DD159 pKa = 3.06RR160 pKa = 11.84FQIIRR165 pKa = 11.84RR166 pKa = 11.84WRR168 pKa = 11.84FQFKK172 pKa = 10.81GNSTRR177 pKa = 11.84NGVAYY182 pKa = 10.18DD183 pKa = 3.73CAKK186 pKa = 10.22NRR188 pKa = 11.84HH189 pKa = 5.84DD190 pKa = 3.67FRR192 pKa = 11.84ANVKK196 pKa = 10.03LNSISEE202 pKa = 4.4FKK204 pKa = 10.87SATDD208 pKa = 3.46GSYY211 pKa = 11.84ANTQKK216 pKa = 10.88NAYY219 pKa = 7.83TMYY222 pKa = 10.4FVPQTYY228 pKa = 11.21DD229 pKa = 3.09MVVDD233 pKa = 4.23GHH235 pKa = 6.18CTMKK239 pKa = 9.86YY240 pKa = 8.42TSIVV244 pKa = 3.18

Molecular weight: 27.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1243

43

330

177.6

20.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.827 ± 0.775

1.931 ± 0.348

5.551 ± 0.685

5.39 ± 1.032

4.183 ± 0.38

5.229 ± 0.795

2.172 ± 0.336

6.275 ± 0.58

5.068 ± 0.854

7.723 ± 0.955

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.496

4.907 ± 0.565

6.356 ± 1.277

4.747 ± 0.443

6.999 ± 0.453

8.528 ± 0.685

6.436 ± 0.488

4.666 ± 0.771

2.333 ± 0.274

4.103 ± 0.391
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski