Wenling tombus-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH34|A0A1L3KH34_9VIRU RNA-directed RNA polymerase OS=Wenling tombus-like virus 4 OX=1923546 PE=4 SV=1
MM1 pKa = 7.84PCMEE5 pKa = 5.44DD6 pKa = 2.8VTMATTVEE14 pKa = 4.24ATDD17 pKa = 3.4SSIWARR23 pKa = 11.84KK24 pKa = 8.33VPHH27 pKa = 6.19SVAIATAEE35 pKa = 4.08QVCLLEE41 pKa = 4.29TVEE44 pKa = 4.32IRR46 pKa = 11.84VPHH49 pKa = 6.7PLHH52 pKa = 6.58PGKK55 pKa = 10.81GLTDD59 pKa = 4.96LNTLAAQFLASKK71 pKa = 9.35PNQWLTQVEE80 pKa = 4.41PTPFQTWVKK89 pKa = 9.52RR90 pKa = 11.84FSPSRR95 pKa = 11.84QTQLEE100 pKa = 3.95AGRR103 pKa = 11.84EE104 pKa = 3.84WVARR108 pKa = 11.84HH109 pKa = 5.68GLLEE113 pKa = 4.65KK114 pKa = 10.7DD115 pKa = 3.44SIMAVFVKK123 pKa = 10.78VEE125 pKa = 4.04TSVTGTDD132 pKa = 3.66PRR134 pKa = 11.84NISPRR139 pKa = 11.84DD140 pKa = 3.41PRR142 pKa = 11.84FLSTLGPFTSRR153 pKa = 11.84LEE155 pKa = 4.02QLAVSCPYY163 pKa = 10.08LVKK166 pKa = 10.88GLDD169 pKa = 3.34TYY171 pKa = 11.6KK172 pKa = 10.57RR173 pKa = 11.84GPLLASYY180 pKa = 8.96WRR182 pKa = 11.84ACVIEE187 pKa = 4.16TDD189 pKa = 4.45FSRR192 pKa = 11.84FDD194 pKa = 3.36MTVSQDD200 pKa = 3.12IINTVEE206 pKa = 3.6RR207 pKa = 11.84SLFRR211 pKa = 11.84RR212 pKa = 11.84CFPEE216 pKa = 4.46GLYY219 pKa = 10.14PDD221 pKa = 5.62LDD223 pKa = 3.98ACLSYY228 pKa = 11.02LDD230 pKa = 4.17TMTGVSDD237 pKa = 3.96LGIMYY242 pKa = 7.69TVSGTRR248 pKa = 11.84ASGDD252 pKa = 3.06AHH254 pKa = 5.77TSIANGYY261 pKa = 8.78INRR264 pKa = 11.84FVIWSCLRR272 pKa = 11.84HH273 pKa = 6.35LDD275 pKa = 3.54PSKK278 pKa = 10.22WDD280 pKa = 3.65SFHH283 pKa = 7.05EE284 pKa = 4.71GDD286 pKa = 5.06DD287 pKa = 3.8GVIFLDD293 pKa = 3.38QDD295 pKa = 3.13IFEE298 pKa = 4.6TARR301 pKa = 11.84EE302 pKa = 4.04CLKK305 pKa = 10.64FASCLGFKK313 pKa = 10.63LKK315 pKa = 10.51VICPEE320 pKa = 4.17SQDD323 pKa = 3.47SAVFCGRR330 pKa = 11.84FTCTTCHH337 pKa = 7.09RR338 pKa = 11.84EE339 pKa = 4.04YY340 pKa = 11.05CDD342 pKa = 4.52LPRR345 pKa = 11.84ALSKK349 pKa = 10.67FHH351 pKa = 6.19TTTKK355 pKa = 10.93NGDD358 pKa = 3.78CKK360 pKa = 11.25SLVLAKK366 pKa = 10.35ALSYY370 pKa = 10.38FATDD374 pKa = 2.36AHH376 pKa = 5.73TPIMGVLCDD385 pKa = 4.56ALIKK389 pKa = 10.62LLSPLVSQRR398 pKa = 11.84LMRR401 pKa = 11.84RR402 pKa = 11.84RR403 pKa = 11.84LHH405 pKa = 6.02TMNWWDD411 pKa = 3.85RR412 pKa = 11.84ARR414 pKa = 11.84VLQGINMTLADD425 pKa = 4.12VSPCCRR431 pKa = 11.84ASVSQTSGFDD441 pKa = 3.33PPLQVAAEE449 pKa = 3.95QQLAKK454 pKa = 9.69WAVGVDD460 pKa = 3.54EE461 pKa = 4.82WNPIAVSDD469 pKa = 4.1FLVDD473 pKa = 4.53IPGQLCVYY481 pKa = 10.34

Molecular weight: 53.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGS1|A0A1L3KGS1_9VIRU Capsid protein OS=Wenling tombus-like virus 4 OX=1923546 PE=3 SV=1
MM1 pKa = 7.41FFQYY5 pKa = 10.98LLPVLTAIGLLRR17 pKa = 11.84GSTTQLDD24 pKa = 4.13HH25 pKa = 7.07TWFEE29 pKa = 4.32WLGLPLYY36 pKa = 10.56FSLPDD41 pKa = 2.92SWQWVSEE48 pKa = 3.7WCEE51 pKa = 3.72YY52 pKa = 11.05LGLKK56 pKa = 8.75TLMGVPDD63 pKa = 4.22ADD65 pKa = 4.0RR66 pKa = 11.84FQDD69 pKa = 5.43LIDD72 pKa = 4.98DD73 pKa = 4.39CTQLEE78 pKa = 4.48VTISSWKK85 pKa = 8.92LAKK88 pKa = 10.39SLGSFSPVGFSNRR101 pKa = 11.84YY102 pKa = 7.31YY103 pKa = 9.83VCKK106 pKa = 10.54VLIGRR111 pKa = 11.84GYY113 pKa = 9.04VTEE116 pKa = 4.06QSAPLLLRR124 pKa = 11.84LMALPWLVIDD134 pKa = 5.32WILSLGPIALIILTTCTIISVCALALYY161 pKa = 9.83VCYY164 pKa = 10.43ILARR168 pKa = 11.84RR169 pKa = 11.84SNIQVACDD177 pKa = 3.7PPPFTHH183 pKa = 7.54RR184 pKa = 11.84EE185 pKa = 3.99VKK187 pKa = 10.48DD188 pKa = 3.21ILCSYY193 pKa = 8.84FAKK196 pKa = 10.79NKK198 pKa = 10.27LDD200 pKa = 3.69VDD202 pKa = 4.39SVDD205 pKa = 4.33PDD207 pKa = 3.74PTGHH211 pKa = 7.23PKK213 pKa = 10.02LAYY216 pKa = 9.33IRR218 pKa = 11.84RR219 pKa = 11.84QMEE222 pKa = 3.7KK223 pKa = 10.7AMQVFLLRR231 pKa = 11.84LNRR234 pKa = 11.84KK235 pKa = 8.61IRR237 pKa = 11.84DD238 pKa = 3.51VGGSLSRR245 pKa = 11.84SAKK248 pKa = 9.99LLKK251 pKa = 10.27QGSKK255 pKa = 10.38HH256 pKa = 5.3ICFPNLTSADD266 pKa = 3.75KK267 pKa = 10.93EE268 pKa = 4.01RR269 pKa = 11.84LEE271 pKa = 3.97LAKK274 pKa = 10.76RR275 pKa = 11.84NLDD278 pKa = 3.39AEE280 pKa = 4.31AWEE283 pKa = 4.97HH284 pKa = 6.55IGLHH288 pKa = 6.3KK289 pKa = 10.77GDD291 pKa = 4.19EE292 pKa = 4.49CPHH295 pKa = 6.72RR296 pKa = 11.84SLPSYY301 pKa = 7.36MTYY304 pKa = 10.86VDD306 pKa = 4.24FHH308 pKa = 7.06LSPEE312 pKa = 4.19QLCRR316 pKa = 11.84TIKK319 pKa = 10.76SPTLILTHH327 pKa = 7.32DD328 pKa = 3.98FAKK331 pKa = 10.36VDD333 pKa = 3.57KK334 pKa = 11.31DD335 pKa = 3.57GEE337 pKa = 4.11QWFNGEE343 pKa = 4.42CDD345 pKa = 2.96IKK347 pKa = 10.86RR348 pKa = 11.84YY349 pKa = 10.49GDD351 pKa = 4.08FISFQTRR358 pKa = 11.84GGLKK362 pKa = 8.7YY363 pKa = 8.8THH365 pKa = 7.27GYY367 pKa = 8.78HH368 pKa = 6.2AWEE371 pKa = 4.31NEE373 pKa = 3.63GTICTTEE380 pKa = 3.66GTLRR384 pKa = 11.84YY385 pKa = 8.9YY386 pKa = 10.23RR387 pKa = 11.84VYY389 pKa = 9.55EE390 pKa = 3.77AHH392 pKa = 6.82GSIIIYY398 pKa = 9.0ACPVAGEE405 pKa = 3.97YY406 pKa = 9.51TPRR409 pKa = 11.84VDD411 pKa = 4.0NTLCSTAGSADD422 pKa = 3.38AYY424 pKa = 9.94KK425 pKa = 10.85LNNGVEE431 pKa = 4.13AHH433 pKa = 5.95VKK435 pKa = 10.42GDD437 pKa = 3.48VVQVGDD443 pKa = 4.19HH444 pKa = 6.8EE445 pKa = 4.53ISKK448 pKa = 8.97DD449 pKa = 3.08TLYY452 pKa = 10.71RR453 pKa = 11.84VAYY456 pKa = 9.91QMSLLPRR463 pKa = 11.84DD464 pKa = 4.34EE465 pKa = 4.83KK466 pKa = 10.68WLPNCSSVLRR476 pKa = 11.84GRR478 pKa = 11.84FTADD482 pKa = 3.01EE483 pKa = 4.37MPASALHH490 pKa = 7.12DD491 pKa = 3.66SLQFVVMLADD501 pKa = 4.28RR502 pKa = 11.84MAVSYY507 pKa = 8.93RR508 pKa = 11.84QSMIGDD514 pKa = 3.55PQSYY518 pKa = 8.28NFLMRR523 pKa = 11.84RR524 pKa = 11.84VLLLVLNLTSRR535 pKa = 11.84LPDD538 pKa = 3.45TLSAPIVAAFRR549 pKa = 11.84SFRR552 pKa = 11.84RR553 pKa = 11.84FLSGKK558 pKa = 8.0SPRR561 pKa = 11.84HH562 pKa = 5.71ALSSWMWTVHH572 pKa = 6.26RR573 pKa = 11.84VPTYY577 pKa = 10.38EE578 pKa = 4.28VMWDD582 pKa = 3.3NVAAMLIDD590 pKa = 3.91PKK592 pKa = 10.75KK593 pKa = 10.17PNSAKK598 pKa = 10.36QPFPFSGATAVAPTVEE614 pKa = 4.45QPSGDD619 pKa = 3.5TSQDD623 pKa = 2.93EE624 pKa = 4.54GKK626 pKa = 9.42PACRR630 pKa = 11.84DD631 pKa = 3.33RR632 pKa = 11.84KK633 pKa = 9.98EE634 pKa = 4.39SSSSCSPPSPGSSGVGKK651 pKa = 9.89SQAILQPAGLPDD663 pKa = 4.71DD664 pKa = 4.7PANLPATTSANTQALPQGGVGAGKK688 pKa = 10.16KK689 pKa = 9.5KK690 pKa = 10.9AEE692 pKa = 4.45TISRR696 pKa = 11.84TICIHH701 pKa = 5.83AVHH704 pKa = 7.05GGRR707 pKa = 11.84NHH709 pKa = 7.37GDD711 pKa = 3.18DD712 pKa = 3.56CRR714 pKa = 11.84GYY716 pKa = 10.41GLQYY720 pKa = 10.54LGKK723 pKa = 9.41EE724 pKa = 4.39GSALRR729 pKa = 11.84RR730 pKa = 11.84DD731 pKa = 3.98RR732 pKa = 11.84NRR734 pKa = 11.84GASVPIGDD742 pKa = 4.4RR743 pKa = 11.84RR744 pKa = 11.84NSGTPPPPSRR754 pKa = 11.84KK755 pKa = 9.35RR756 pKa = 11.84ADD758 pKa = 3.76RR759 pKa = 11.84SQHH762 pKa = 5.11TGSTISGEE770 pKa = 4.26QTKK773 pKa = 11.21SMVDD777 pKa = 3.15SSGANSLPDD786 pKa = 3.63VGQKK790 pKa = 9.6ILPVKK795 pKa = 9.56TNAARR800 pKa = 11.84GGKK803 pKa = 9.58GVGGATRR810 pKa = 11.84PVGKK814 pKa = 9.98GFHH817 pKa = 6.37NGSVRR822 pKa = 11.84QGGDD826 pKa = 2.88VSHH829 pKa = 7.31RR830 pKa = 11.84NGSSQYY836 pKa = 10.74KK837 pKa = 8.71PARR840 pKa = 11.84SS841 pKa = 3.28

Molecular weight: 92.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1793

471

841

597.7

66.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.362 ± 0.07

2.398 ± 0.653

6.525 ± 0.402

3.625 ± 0.34

3.346 ± 0.45

7.418 ± 0.892

2.175 ± 0.438

4.294 ± 0.069

4.573 ± 0.375

9.091 ± 0.826

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.181

3.123 ± 0.51

5.968 ± 0.214

4.239 ± 0.545

6.358 ± 0.2

8.087 ± 0.631

7.25 ± 1.109

6.972 ± 0.5

1.785 ± 0.213

3.235 ± 0.434
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski