Gordonia phage GRU3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0K0N694|A0A0K0N694_9CAUD Uncharacterized protein OS=Gordonia phage GRU3 OX=1647473 GN=GRU3_24 PE=4 SV=1
MM1 pKa = 7.14STLTDD6 pKa = 3.36ALGRR10 pKa = 11.84VVDD13 pKa = 4.34ALTAAGIAATTNPADD28 pKa = 4.04VIAPGAWVIPHH39 pKa = 7.6DD40 pKa = 3.68ITDD43 pKa = 3.67PTLCGGLTVRR53 pKa = 11.84ADD55 pKa = 3.6VCLVAPDD62 pKa = 4.19NGTANAIAILGDD74 pKa = 4.37LLDD77 pKa = 3.66QAAPVLTFDD86 pKa = 3.56EE87 pKa = 5.23PVRR90 pKa = 11.84SMAVTPPGLSALPALVITTTTDD112 pKa = 2.52

Molecular weight:
11.2 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0K0N6J0|A0A0K0N6J0_9CAUD Uncharacterized protein OS=Gordonia phage GRU3 OX=1647473 GN=GRU3_22 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.24GATRR6 pKa = 11.84SRR8 pKa = 11.84FSPKK12 pKa = 10.27VIAQQADD19 pKa = 3.71RR20 pKa = 11.84AEE22 pKa = 4.97LDD24 pKa = 3.55DD25 pKa = 5.08CSDD28 pKa = 3.29MFTWSAAIAAFISHH42 pKa = 7.1LSAGGRR48 pKa = 11.84ARR50 pKa = 11.84GTRR53 pKa = 11.84EE54 pKa = 3.35LRR56 pKa = 11.84RR57 pKa = 11.84YY58 pKa = 9.7HH59 pKa = 6.82LLCFAEE65 pKa = 4.59VVEE68 pKa = 4.58PLSPLAVTVTDD79 pKa = 4.12LEE81 pKa = 4.24AWLGRR86 pKa = 11.84EE87 pKa = 3.84DD88 pKa = 2.96WTANTRR94 pKa = 11.84RR95 pKa = 11.84SARR98 pKa = 11.84STLVGFFSWVSANGWRR114 pKa = 11.84ADD116 pKa = 3.54NPAALLATVTGVLGRR131 pKa = 11.84PRR133 pKa = 11.84PCPEE137 pKa = 3.32QFLRR141 pKa = 11.84SAVIAAGPRR150 pKa = 11.84EE151 pKa = 4.27RR152 pKa = 11.84LMLALGAGCGLRR164 pKa = 11.84RR165 pKa = 11.84GEE167 pKa = 4.04IARR170 pKa = 11.84VRR172 pKa = 11.84GDD174 pKa = 3.53HH175 pKa = 7.09VEE177 pKa = 4.04DD178 pKa = 4.96TIDD181 pKa = 3.87GPILRR186 pKa = 11.84VLGKK190 pKa = 10.34GNKK193 pKa = 7.92TRR195 pKa = 11.84TVPISDD201 pKa = 3.82DD202 pKa = 2.82MAMRR206 pKa = 11.84LRR208 pKa = 11.84EE209 pKa = 3.83RR210 pKa = 11.84AGYY213 pKa = 10.08AFPSPRR219 pKa = 11.84GGHH222 pKa = 5.94LTPAHH227 pKa = 5.76VGKK230 pKa = 10.26LVSRR234 pKa = 11.84LLPPGWTTHH243 pKa = 4.72TLRR246 pKa = 11.84HH247 pKa = 5.89RR248 pKa = 11.84FASAAYY254 pKa = 9.79RR255 pKa = 11.84ADD257 pKa = 3.86RR258 pKa = 11.84DD259 pKa = 3.24IRR261 pKa = 11.84AVQEE265 pKa = 4.27LLGHH269 pKa = 6.66ASVATTQIYY278 pKa = 7.84TAIPDD283 pKa = 3.83DD284 pKa = 3.59ATRR287 pKa = 11.84RR288 pKa = 11.84ASLAAAIVAA297 pKa = 4.31

Molecular weight:
32.29 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

5377

34

656

206.8

22.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.116 ± 0.795

0.763 ± 0.151

6.379 ± 0.609

4.036 ± 0.351

2.808 ± 0.18

8.369 ± 0.328

1.804 ± 0.255

5.133 ± 0.528

2.827 ± 0.26

7.532 ± 0.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.174

3.199 ± 0.374

6.156 ± 0.524

2.548 ± 0.238

6.974 ± 0.725

5.505 ± 0.278

7.104 ± 0.42

8.22 ± 0.559

2.12 ± 0.28

2.232 ± 0.268

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski