Bifidobacterium dolichotidis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430FSH8|A0A430FSH8_9BIFI Alpha/beta hydrolase fold protein OS=Bifidobacterium dolichotidis OX=2306976 GN=D2E26_0391 PE=4 SV=1
MM1 pKa = 7.55CHH3 pKa = 6.28TNNHH7 pKa = 5.06QQTMTQDD14 pKa = 3.08QGASSDD20 pKa = 3.6GDD22 pKa = 3.14KK23 pKa = 11.21DD24 pKa = 3.89FQNEE28 pKa = 3.54ATQQDD33 pKa = 4.1EE34 pKa = 4.29QQEE37 pKa = 4.2QSQQGQQGQQSEE49 pKa = 4.55QPQHH53 pKa = 6.87CKK55 pKa = 8.12QQHH58 pKa = 5.23GNMHH62 pKa = 6.26SEE64 pKa = 3.82ASVQNGVGGTAADD77 pKa = 4.06DD78 pKa = 3.8QADD81 pKa = 3.51AQYY84 pKa = 11.35LVDD87 pKa = 3.45QHH89 pKa = 7.06AGSEE93 pKa = 4.23DD94 pKa = 3.39AGVAEE99 pKa = 5.65NGDD102 pKa = 3.7QSQQPMQQPNGDD114 pKa = 3.65GTGVMSPEE122 pKa = 4.26DD123 pKa = 3.59PRR125 pKa = 11.84GEE127 pKa = 4.18GSLEE131 pKa = 4.2AIANGTGAEE140 pKa = 4.02QGSASEE146 pKa = 3.94QSGPNTANGQHH157 pKa = 6.4CMQHH161 pKa = 5.5MNAA164 pKa = 4.57

Molecular weight: 17.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430FNZ2|A0A430FNZ2_9BIFI ATPase OS=Bifidobacterium dolichotidis OX=2306976 GN=D2E26_1360 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.79MKK15 pKa = 9.36HH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84KK40 pKa = 8.81SLAAA44 pKa = 4.22

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1448

0

1448

539544

29

1873

372.6

40.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.607 ± 0.073

0.978 ± 0.021

6.158 ± 0.049

5.941 ± 0.062

3.471 ± 0.039

7.112 ± 0.059

2.477 ± 0.033

5.623 ± 0.043

4.013 ± 0.051

8.781 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.029

3.75 ± 0.041

4.513 ± 0.036

4.418 ± 0.048

5.329 ± 0.056

6.212 ± 0.049

6.192 ± 0.047

7.63 ± 0.053

1.301 ± 0.029

2.669 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski