Mycobacterium phage Indlulamithi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Indlulamithivirus; Mycobacterium virus Indlulamithi

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VCJ1|A0A649VCJ1_9CAUD Uncharacterized protein OS=Mycobacterium phage Indlulamithi OX=2656582 GN=29 PE=4 SV=1
MM1 pKa = 7.28TAPTNATWAVSYY13 pKa = 9.07RR14 pKa = 11.84APGIDD19 pKa = 4.25PEE21 pKa = 4.41NPDD24 pKa = 3.56DD25 pKa = 4.4LFRR28 pKa = 11.84LVFICEE34 pKa = 4.06KK35 pKa = 10.34GQEE38 pKa = 3.84QAIRR42 pKa = 11.84VRR44 pKa = 11.84DD45 pKa = 3.76MYY47 pKa = 11.02ATMGMGCEE55 pKa = 4.05VLYY58 pKa = 8.63ATPEE62 pKa = 3.93IDD64 pKa = 2.85WQLFGEE70 pKa = 4.57DD71 pKa = 3.77DD72 pKa = 3.97GTPVVYY78 pKa = 10.38EE79 pKa = 4.52PGTDD83 pKa = 3.64NPLDD87 pKa = 4.09PNAVVAEE94 pKa = 4.6DD95 pKa = 4.39DD96 pKa = 4.47SGDD99 pKa = 3.66EE100 pKa = 4.18PTTT103 pKa = 3.66

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VCV9|A0A649VCV9_9CAUD Uncharacterized protein OS=Mycobacterium phage Indlulamithi OX=2656582 GN=109 PE=4 SV=1
MM1 pKa = 7.31GKK3 pKa = 9.37VRR5 pKa = 11.84SKK7 pKa = 11.13AQWRR11 pKa = 11.84WMFANKK17 pKa = 9.98KK18 pKa = 9.54SFARR22 pKa = 11.84RR23 pKa = 11.84WAHH26 pKa = 5.29ASKK29 pKa = 10.65GKK31 pKa = 10.34AYY33 pKa = 9.72KK34 pKa = 10.25RR35 pKa = 11.84LPKK38 pKa = 9.99KK39 pKa = 9.9VRR41 pKa = 11.84KK42 pKa = 7.01TKK44 pKa = 10.12KK45 pKa = 8.39SAGRR49 pKa = 11.84RR50 pKa = 11.84VKK52 pKa = 10.56RR53 pKa = 3.69

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

111

0

111

22191

34

1862

199.9

22.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.877 ± 0.386

0.964 ± 0.149

7.165 ± 0.225

6.25 ± 0.393

3.56 ± 0.131

8.066 ± 0.487

1.915 ± 0.164

5.372 ± 0.185

5.403 ± 0.291

7.377 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.146

4.421 ± 0.197

5.214 ± 0.15

4.286 ± 0.278

5.854 ± 0.329

5.827 ± 0.186

5.746 ± 0.224

6.453 ± 0.184

1.812 ± 0.156

3.069 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski