Pseudomonas phage phi6 (Bacteriophage phi-6)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes; Mindivirales; Cystoviridae; Cystovirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P11124|RDRP_BPPH6 RNA-directed RNA polymerase OS=Pseudomonas phage phi6 OX=10879 GN=P2 PE=1 SV=3
MM1 pKa = 7.39TLYY4 pKa = 10.67LVPPLDD10 pKa = 4.0SADD13 pKa = 3.84KK14 pKa = 10.13EE15 pKa = 4.4LPALASKK22 pKa = 10.95AGVTLLEE29 pKa = 4.02IEE31 pKa = 4.77FLHH34 pKa = 6.24EE35 pKa = 4.44LWPHH39 pKa = 6.23LSGGQIVIAALNANNLAILNRR60 pKa = 11.84HH61 pKa = 5.44MSTLLVEE68 pKa = 4.65LPVAVMAVPGASYY81 pKa = 10.62RR82 pKa = 11.84SDD84 pKa = 2.83WNMIAHH90 pKa = 7.69ALPSEE95 pKa = 4.06DD96 pKa = 4.97WITLSNKK103 pKa = 7.22MLKK106 pKa = 10.24SGLLANDD113 pKa = 3.88TVQGEE118 pKa = 4.4KK119 pKa = 10.42RR120 pKa = 11.84SGAEE124 pKa = 3.51PLSPNVYY131 pKa = 9.32TDD133 pKa = 3.04ALSRR137 pKa = 11.84LGIATAHH144 pKa = 7.27AIPVEE149 pKa = 4.1PEE151 pKa = 3.57QPFDD155 pKa = 3.81VDD157 pKa = 3.61EE158 pKa = 4.81VSAA161 pKa = 4.47

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q38563|P14_BPPH6 Protein P14 OS=Pseudomonas phage phi6 OX=10879 GN=P14 PE=4 SV=1
MM1 pKa = 7.33VPLKK5 pKa = 10.56ISTLEE10 pKa = 4.04SQLQPLVKK18 pKa = 10.25LVATEE23 pKa = 4.17TPGALVAYY31 pKa = 10.15ARR33 pKa = 11.84GLSSADD39 pKa = 3.26RR40 pKa = 11.84SRR42 pKa = 11.84LYY44 pKa = 10.62RR45 pKa = 11.84LLRR48 pKa = 11.84SLEE51 pKa = 3.73QAIPKK56 pKa = 9.95LSSAVVSATTLAARR70 pKa = 11.84GLL72 pKa = 3.84

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

3573

42

769

274.8

29.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.867 ± 0.696

0.756 ± 0.217

5.29 ± 0.488

4.786 ± 0.634

3.554 ± 0.378

7.389 ± 0.636

1.707 ± 0.261

5.01 ± 0.571

4.282 ± 0.377

9.684 ± 0.57

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.277

3.387 ± 0.401

4.898 ± 0.284

3.722 ± 0.497

5.122 ± 0.639

7.305 ± 0.349

5.709 ± 0.475

8.2 ± 0.702

1.539 ± 0.279

3.051 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski