Escherichia phage Mu (Bacteriophage Mu)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Muvirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9T1V4|BP45_BPMU Baseplate puncturing device gp45 OS=Escherichia phage Mu OX=10677 GN=Mup45 PE=1 SV=1
MM1 pKa = 7.68TDD3 pKa = 4.66LAIIWTNGRR12 pKa = 11.84GDD14 pKa = 3.51IAQDD18 pKa = 4.38GIDD21 pKa = 4.03MLTDD25 pKa = 3.93DD26 pKa = 5.32SLTTDD31 pKa = 3.15VTISLFTDD39 pKa = 3.69RR40 pKa = 11.84RR41 pKa = 11.84ALDD44 pKa = 3.79SDD46 pKa = 4.03TLPDD50 pKa = 3.86GSDD53 pKa = 4.3DD54 pKa = 4.28RR55 pKa = 11.84RR56 pKa = 11.84GWWGDD61 pKa = 3.27SYY63 pKa = 11.52RR64 pKa = 11.84DD65 pKa = 3.52RR66 pKa = 11.84PIGSRR71 pKa = 11.84LWLLSRR77 pKa = 11.84EE78 pKa = 4.06KK79 pKa = 10.11ATPDD83 pKa = 3.03TLEE86 pKa = 3.9RR87 pKa = 11.84ARR89 pKa = 11.84GYY91 pKa = 11.0AEE93 pKa = 5.35EE94 pKa = 4.03ALEE97 pKa = 4.01WLKK100 pKa = 9.71TAGRR104 pKa = 11.84VSAINVRR111 pKa = 11.84AEE113 pKa = 3.82QLHH116 pKa = 5.41QGWLYY121 pKa = 11.05LYY123 pKa = 10.13IALTLPDD130 pKa = 4.04GSVIPYY136 pKa = 8.87EE137 pKa = 4.41FKK139 pKa = 10.91AAFNGVV145 pKa = 3.07

Molecular weight: 16.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03763|TARGB_BPMU ATP-dependent target DNA activator B OS=Escherichia phage Mu OX=10677 GN=B PE=1 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84NIKK6 pKa = 9.16MATDD10 pKa = 3.59AQNWLQARR18 pKa = 11.84GSHH21 pKa = 5.44VNEE24 pKa = 4.58SYY26 pKa = 11.04LGVARR31 pKa = 11.84PILEE35 pKa = 3.65ITYY38 pKa = 9.73PPVEE42 pKa = 4.04LVKK45 pKa = 10.55NAVRR49 pKa = 11.84IMEE52 pKa = 4.6HH53 pKa = 6.22KK54 pKa = 10.57SGVARR59 pKa = 11.84SVWTARR65 pKa = 11.84LNGCQIIWRR74 pKa = 4.14

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

1

56

12031

39

690

214.8

23.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.199 ± 0.432

1.189 ± 0.161

5.818 ± 0.279

6.392 ± 0.363

2.942 ± 0.157

7.506 ± 0.356

1.521 ± 0.138

5.245 ± 0.246

4.804 ± 0.293

9.085 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.793 ± 0.171

4.289 ± 0.406

3.965 ± 0.165

4.33 ± 0.198

7.165 ± 0.319

5.81 ± 0.278

6.076 ± 0.344

6.334 ± 0.245

1.895 ± 0.133

2.643 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski