Xanthomonas phage phiLf (Bacteriophage phi-Lf)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q38617|REP_BPPHL Replication-associated protein G2P OS=Xanthomonas phage phiLf OX=28365 GN=II PE=3 SV=1
MM1 pKa = 7.39SPSLFLGWGDD11 pKa = 4.17DD12 pKa = 3.8YY13 pKa = 11.65FVVLWIHH20 pKa = 5.71GRR22 pKa = 11.84AVRR25 pKa = 11.84LGRR28 pKa = 11.84RR29 pKa = 11.84QGVGRR34 pKa = 11.84VIRR37 pKa = 11.84VSLAMLILLALTFSPIVHH55 pKa = 6.21ATCVQTEE62 pKa = 4.21PSTSANNGSWACSDD76 pKa = 3.37QGEE79 pKa = 4.47AFAKK83 pKa = 9.55ASSMGVPADD92 pKa = 4.24LSVCRR97 pKa = 11.84MKK99 pKa = 10.71SIRR102 pKa = 11.84AVSSGPGVFSQRR114 pKa = 11.84MTYY117 pKa = 10.29PGDD120 pKa = 3.51TCGIGYY126 pKa = 9.9DD127 pKa = 4.05LDD129 pKa = 4.73IGTGNATYY137 pKa = 10.39PDD139 pKa = 3.88TATCAKK145 pKa = 10.15RR146 pKa = 11.84PSQSGWTNPTAPTPSDD162 pKa = 3.5VCNDD166 pKa = 2.92GCYY169 pKa = 8.73YY170 pKa = 10.45TYY172 pKa = 11.14AVDD175 pKa = 3.65AGGPKK180 pKa = 10.35GYY182 pKa = 9.08TYY184 pKa = 10.92VPSGATCTTDD194 pKa = 4.31DD195 pKa = 4.42AAPPIDD201 pKa = 5.37DD202 pKa = 5.32GGDD205 pKa = 3.45GDD207 pKa = 5.9DD208 pKa = 5.68DD209 pKa = 4.77GGGDD213 pKa = 3.56GGGDD217 pKa = 3.3GGGDD221 pKa = 3.3GGGDD225 pKa = 3.3GGGDD229 pKa = 3.3GGGDD233 pKa = 3.3GGGDD237 pKa = 3.3GGGDD241 pKa = 3.3GGGDD245 pKa = 3.25GGGDD249 pKa = 3.53GDD251 pKa = 5.0GGGDD255 pKa = 3.82GDD257 pKa = 5.14GDD259 pKa = 4.06GDD261 pKa = 4.01GDD263 pKa = 4.09GDD265 pKa = 4.05GEE267 pKa = 4.39EE268 pKa = 4.66GGEE271 pKa = 4.07GAPMSEE277 pKa = 4.89LYY279 pKa = 10.33KK280 pKa = 10.79KK281 pKa = 10.4SGKK284 pKa = 7.4TVEE287 pKa = 4.37SVLSKK292 pKa = 10.98FNTQVRR298 pKa = 11.84GTPMVAGIGDD308 pKa = 4.1FMKK311 pKa = 10.52VPSGGSCPVFSLGASKK327 pKa = 9.08WWDD330 pKa = 2.73AMTINFHH337 pKa = 6.92CGGDD341 pKa = 3.5FLAFLRR347 pKa = 11.84AAGWVILAIAAYY359 pKa = 9.15AAIRR363 pKa = 11.84IAVTT367 pKa = 3.32

Molecular weight: 36.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q38617-2|REP-2_BPPHL Isoform of Q38617 Isoform G10P of Replication-associated protein G2P OS=Xanthomonas phage phiLf OX=28365 GN=II PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84GHH4 pKa = 7.07FNRR7 pKa = 11.84LKK9 pKa = 10.44SGRR12 pKa = 11.84ARR14 pKa = 11.84WNRR17 pKa = 11.84EE18 pKa = 3.23SFRR21 pKa = 11.84YY22 pKa = 9.38VWVGEE27 pKa = 3.78LTQRR31 pKa = 11.84FRR33 pKa = 11.84PHH35 pKa = 4.67YY36 pKa = 10.3HH37 pKa = 5.23VMLWVPQGMFFGKK50 pKa = 9.92VDD52 pKa = 3.46QRR54 pKa = 11.84GWWPHH59 pKa = 5.12GSSQIEE65 pKa = 4.12KK66 pKa = 10.46ARR68 pKa = 11.84NCVGYY73 pKa = 9.54LAKK76 pKa = 10.57YY77 pKa = 9.55ASKK80 pKa = 9.13FTAITAAAFPKK91 pKa = 10.2GFRR94 pKa = 11.84THH96 pKa = 6.56GCGGLNTEE104 pKa = 4.92SKK106 pKa = 10.88RR107 pKa = 11.84EE108 pKa = 3.99LRR110 pKa = 11.84WWKK113 pKa = 10.13APKK116 pKa = 9.76DD117 pKa = 3.52ARR119 pKa = 11.84EE120 pKa = 4.1ALGGEE125 pKa = 3.86ADD127 pKa = 3.2IRR129 pKa = 11.84KK130 pKa = 9.88AKK132 pKa = 10.54GGWFDD137 pKa = 3.86RR138 pKa = 11.84LTGEE142 pKa = 4.94FWPSPWKK149 pKa = 9.18VTFIFGRR156 pKa = 11.84TFAWKK161 pKa = 10.04VVQLL165 pKa = 4.0

Molecular weight: 19.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

2

10

1825

38

440

182.5

19.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.74 ± 0.924

1.589 ± 0.488

5.973 ± 1.152

3.616 ± 0.562

4.548 ± 0.806

12.164 ± 2.123

1.534 ± 0.33

3.945 ± 0.464

4.822 ± 0.749

6.411 ± 0.797

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.63 ± 0.482

2.466 ± 0.422

5.26 ± 0.543

3.562 ± 0.571

6.63 ± 1.078

5.973 ± 0.533

6.027 ± 0.436

6.74 ± 0.46

2.74 ± 0.673

2.63 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski