Enterococcus phage vB_EfaS_IME197

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2MYJ7|A0A0S2MYJ7_9CAUD YopX domain-containing protein OS=Enterococcus phage vB_EfaS_IME197 OX=1747326 PE=4 SV=1
MM1 pKa = 7.58LANEE5 pKa = 4.55YY6 pKa = 10.32FDD8 pKa = 3.66QSSEE12 pKa = 4.15EE13 pKa = 4.0VDD15 pKa = 3.77SDD17 pKa = 5.55EE18 pKa = 4.19LACWIDD24 pKa = 3.46GNADD28 pKa = 3.56LFFNAWVCGYY38 pKa = 9.11EE39 pKa = 4.06VEE41 pKa = 5.08KK42 pKa = 10.72EE43 pKa = 3.62QLYY46 pKa = 9.72YY47 pKa = 11.23VSFGFDD53 pKa = 2.55RR54 pKa = 11.84FLIKK58 pKa = 10.45GDD60 pKa = 3.85CKK62 pKa = 9.27TTTGEE67 pKa = 3.8DD68 pKa = 4.3FIWIEE73 pKa = 3.71VWEE76 pKa = 4.75CIEE79 pKa = 4.13NEE81 pKa = 4.11GFEE84 pKa = 4.26EE85 pKa = 4.24LVKK88 pKa = 10.22FTEE91 pKa = 4.15QEE93 pKa = 3.36IKK95 pKa = 10.75AIDD98 pKa = 3.54EE99 pKa = 4.69RR100 pKa = 11.84YY101 pKa = 8.76WPFAVKK107 pKa = 10.4VGEE110 pKa = 4.2EE111 pKa = 3.86

Molecular weight: 13.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2MYF0|A0A0S2MYF0_9CAUD Capsid and scaffold protein OS=Enterococcus phage vB_EfaS_IME197 OX=1747326 PE=4 SV=1
MM1 pKa = 7.63ARR3 pKa = 11.84NQVRR7 pKa = 11.84INGLAGISKK16 pKa = 10.06KK17 pKa = 10.56LKK19 pKa = 10.57RR20 pKa = 11.84NAQLDD25 pKa = 3.93DD26 pKa = 3.58VKK28 pKa = 11.14KK29 pKa = 10.31VVRR32 pKa = 11.84NNTAEE37 pKa = 4.08LTANMQAEE45 pKa = 4.34AGKK48 pKa = 10.5VLTGHH53 pKa = 6.39WEE55 pKa = 3.96GKK57 pKa = 10.35KK58 pKa = 9.82FVKK61 pKa = 8.91PTGATKK67 pKa = 10.42RR68 pKa = 11.84SIVMRR73 pKa = 11.84LSNNGFSGHH82 pKa = 6.26TGPGTEE88 pKa = 4.04YY89 pKa = 11.04APYY92 pKa = 9.88LINGTRR98 pKa = 11.84FMVKK102 pKa = 9.2RR103 pKa = 11.84DD104 pKa = 3.69FFLPPLKK111 pKa = 9.69QQKK114 pKa = 9.72VKK116 pKa = 10.51FRR118 pKa = 11.84TDD120 pKa = 3.28LEE122 pKa = 4.32RR123 pKa = 11.84LMKK126 pKa = 10.6

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12249

43

1135

182.8

20.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.156 ± 0.461

0.661 ± 0.119

6.115 ± 0.25

7.821 ± 0.374

4.008 ± 0.298

5.494 ± 0.314

1.323 ± 0.174

7.348 ± 0.259

9.364 ± 0.491

7.935 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.2

6.547 ± 0.325

2.808 ± 0.19

4.066 ± 0.195

3.772 ± 0.282

6.76 ± 0.457

6.205 ± 0.361

5.96 ± 0.232

1.061 ± 0.126

4.033 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski