Streptococcus phage phiNJ3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0RXB6|A0A1B0RXB6_9CAUD Uncharacterized protein OS=Streptococcus phage phiNJ3 OX=1701259 GN=phiNJ3_10 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.52LKK4 pKa = 10.66DD5 pKa = 3.35ILEE8 pKa = 4.55LGTYY12 pKa = 9.43GFNPDD17 pKa = 3.62CKK19 pKa = 10.98VEE21 pKa = 4.13IFNMDD26 pKa = 3.22NFEE29 pKa = 4.08EE30 pKa = 4.3RR31 pKa = 11.84LEE33 pKa = 4.14NEE35 pKa = 4.24GFDD38 pKa = 5.31EE39 pKa = 5.48ILIPQNDD46 pKa = 4.22DD47 pKa = 3.14VTIYY51 pKa = 10.18PYY53 pKa = 11.43AFLIEE58 pKa = 4.24DD59 pKa = 4.28SILIAMTEE67 pKa = 3.91EE68 pKa = 4.47DD69 pKa = 5.42DD70 pKa = 4.4NTNEE74 pKa = 4.2CC75 pKa = 4.51

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0RXD0|A0A1B0RXD0_9CAUD Uncharacterized protein OS=Streptococcus phage phiNJ3 OX=1701259 GN=phiNJ3_38 PE=4 SV=1
MM1 pKa = 7.74PRR3 pKa = 11.84RR4 pKa = 11.84PSTPCKK10 pKa = 10.41QNGCPNLVPYY20 pKa = 8.56GHH22 pKa = 7.39KK23 pKa = 10.79YY24 pKa = 10.02CDD26 pKa = 3.26NHH28 pKa = 6.74KK29 pKa = 11.26SNLQLEE35 pKa = 4.64AKK37 pKa = 8.25STKK40 pKa = 10.13AKK42 pKa = 10.25GYY44 pKa = 9.42NGQWTKK50 pKa = 11.25ARR52 pKa = 11.84LRR54 pKa = 11.84YY55 pKa = 10.0LKK57 pKa = 9.81VHH59 pKa = 6.46PLCVQCKK66 pKa = 9.75AKK68 pKa = 10.52GRR70 pKa = 11.84LTKK73 pKa = 10.1ATVVDD78 pKa = 5.28HH79 pKa = 6.23ITPHH83 pKa = 6.87RR84 pKa = 11.84GDD86 pKa = 4.29QEE88 pKa = 4.75RR89 pKa = 11.84FWNQTNWQALCKK101 pKa = 9.93SCHH104 pKa = 5.64DD105 pKa = 4.22RR106 pKa = 11.84KK107 pKa = 8.93TKK109 pKa = 8.13TTDD112 pKa = 2.58RR113 pKa = 11.84YY114 pKa = 10.6VEE116 pKa = 3.73YY117 pKa = 9.41TYY119 pKa = 11.18RR120 pKa = 11.84FF121 pKa = 3.55

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

16336

42

1089

263.5

29.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.489 ± 0.358

0.955 ± 0.132

5.791 ± 0.312

7.413 ± 0.34

3.967 ± 0.174

6.201 ± 0.243

1.947 ± 0.133

7.027 ± 0.335

7.291 ± 0.255

9.078 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.161

4.701 ± 0.174

3.116 ± 0.195

4.346 ± 0.2

4.689 ± 0.209

6.795 ± 0.31

6.342 ± 0.441

6.342 ± 0.219

1.286 ± 0.093

3.801 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski