Rutstroemia sp. NJR-2017a BVV2

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Rutstroemiaceae; Rutstroemia; unclassified Rutstroemia

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8646 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S7PL44|A0A2S7PL44_9HELO Aldo keto reductase protein OS=Rutstroemia sp. NJR-2017a BVV2 OX=2070413 GN=CJF31_00007056 PE=4 SV=1
MM1 pKa = 7.46AFEE4 pKa = 5.35SGTAYY9 pKa = 10.03PEE11 pKa = 3.8SDD13 pKa = 3.68ADD15 pKa = 3.96DD16 pKa = 4.2EE17 pKa = 4.84YY18 pKa = 11.48EE19 pKa = 4.35RR20 pKa = 11.84SVHH23 pKa = 6.09NSSPVLANDD32 pKa = 3.72SDD34 pKa = 4.18ADD36 pKa = 3.87SDD38 pKa = 4.07GLSSSEE44 pKa = 4.25HH45 pKa = 5.86TPTTYY50 pKa = 11.0GNHH53 pKa = 6.24GFGDD57 pKa = 4.21TLPEE61 pKa = 4.09ANITEE66 pKa = 4.35WTADD70 pKa = 3.41DD71 pKa = 4.23CADD74 pKa = 3.65FVGSLGLRR82 pKa = 11.84QYY84 pKa = 11.7GDD86 pKa = 3.27QFLGMALFRR95 pKa = 11.84MVV97 pKa = 3.16

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S7NR91|A0A2S7NR91_9HELO Dihydroxy-acid dehydratase protein OS=Rutstroemia sp. NJR-2017a BVV2 OX=2070413 GN=CJF31_00007440 PE=3 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84RR3 pKa = 11.84SPLMRR8 pKa = 11.84RR9 pKa = 11.84IFPLMRR15 pKa = 11.84RR16 pKa = 11.84IFPLMRR22 pKa = 11.84RR23 pKa = 11.84IFLLMMKK30 pKa = 9.25RR31 pKa = 11.84TLLMRR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.29RR39 pKa = 11.84ALMRR43 pKa = 11.84KK44 pKa = 9.23KK45 pKa = 10.33NLLIGKK51 pKa = 9.43RR52 pKa = 11.84IMIPTRR58 pKa = 11.84LISTGPHH65 pKa = 4.72QRR67 pKa = 11.84LKK69 pKa = 10.95VPSLGAIPRR78 pKa = 11.84GVQKK82 pKa = 10.47IDD84 pKa = 3.26GSVV87 pKa = 2.51

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8646

0

8646

4317793

8

6066

499.4

55.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.169 ± 0.021

1.172 ± 0.01

5.488 ± 0.019

6.409 ± 0.033

3.716 ± 0.017

7.003 ± 0.025

2.263 ± 0.011

5.261 ± 0.02

5.257 ± 0.028

8.786 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.009

3.952 ± 0.015

5.779 ± 0.028

3.77 ± 0.018

5.727 ± 0.026

8.559 ± 0.032

6.165 ± 0.02

6.008 ± 0.02

1.439 ± 0.009

2.855 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski