Photobacterium damselae subsp. piscicida DI21

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Photobacterium; Photobacterium damselae; Photobacterium damselae subsp. piscicida

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0PS95|A0A0K0PS95_PHODP Fimbrial usher family protein OS=Photobacterium damselae subsp. piscicida DI21 OX=1200302 PE=4 SV=1
MM1 pKa = 7.39TMNKK5 pKa = 9.65VILATAILVTTSTAHH20 pKa = 6.76AQTQDD25 pKa = 3.11TADD28 pKa = 3.29ISVSGSINAANTCTISVDD46 pKa = 3.5GGGTLDD52 pKa = 3.92AGSHH56 pKa = 4.53NTVDD60 pKa = 4.28LVSTGDD66 pKa = 3.59RR67 pKa = 11.84ISEE70 pKa = 3.96FKK72 pKa = 10.75EE73 pKa = 3.8FNATVGCTFPTAIAVKK89 pKa = 8.71YY90 pKa = 8.57TSSLPPQPDD99 pKa = 2.68KK100 pKa = 11.16SYY102 pKa = 11.48RR103 pKa = 11.84LGAYY107 pKa = 6.95QTNSGKK113 pKa = 9.96VAAHH117 pKa = 7.03LYY119 pKa = 10.07AAIGGSNAPTAGGVDD134 pKa = 3.31QAFAAIGDD142 pKa = 3.97QDD144 pKa = 4.64LSTISTSSTLTPAADD159 pKa = 3.65GTPISGVVGDD169 pKa = 4.29SRR171 pKa = 11.84NIYY174 pKa = 8.41TVIDD178 pKa = 3.89ANNNPVAATAFVLPFKK194 pKa = 10.74LGVWSDD200 pKa = 3.71DD201 pKa = 3.77TSSGWLDD208 pKa = 4.93DD209 pKa = 3.58IAGSSFSLSSTVTLEE224 pKa = 3.75LHH226 pKa = 6.95SII228 pKa = 4.01

Molecular weight: 23.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0PS63|A0A0K0PS63_PHODP Uncharacterized protein OS=Photobacterium damselae subsp. piscicida DI21 OX=1200302 PE=4 SV=1
MM1 pKa = 7.13YY2 pKa = 10.38SSYY5 pKa = 11.05LPRR8 pKa = 11.84EE9 pKa = 3.42NHH11 pKa = 7.0IIGKK15 pKa = 9.67RR16 pKa = 11.84YY17 pKa = 7.72RR18 pKa = 11.84QRR20 pKa = 11.84IEE22 pKa = 3.86RR23 pKa = 11.84TNLTLRR29 pKa = 11.84SRR31 pKa = 11.84LKK33 pKa = 10.04RR34 pKa = 11.84LVRR37 pKa = 11.84KK38 pKa = 8.12TIGFSKK44 pKa = 10.42SEE46 pKa = 3.92EE47 pKa = 3.94MHH49 pKa = 6.93DD50 pKa = 3.66KK51 pKa = 11.17VIGTFIEE58 pKa = 4.23RR59 pKa = 11.84EE60 pKa = 4.17FYY62 pKa = 10.52HH63 pKa = 7.02

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

34085

40

3994

309.9

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.194 ± 0.364

1.3 ± 0.098

5.246 ± 0.169

5.278 ± 0.152

3.949 ± 0.148

5.809 ± 0.16

2.752 ± 0.154

6.701 ± 0.29

6.167 ± 0.409

10.406 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.101

5.032 ± 0.288

3.993 ± 0.173

5.158 ± 0.389

4.615 ± 0.205

7.502 ± 0.223

5.956 ± 0.148

5.832 ± 0.152

1.326 ± 0.085

3.55 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski