Paludibacterium purpuratum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Chromobacteriaceae; Paludibacterium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V3DVD3|A0A4V3DVD3_9NEIS HxlR family transcriptional regulator OS=Paludibacterium purpuratum OX=1144873 GN=DFP86_106232 PE=4 SV=1
MM1 pKa = 7.53TEE3 pKa = 4.04TTEE6 pKa = 3.99MLSPINFTDD15 pKa = 3.82SACSKK20 pKa = 9.8VRR22 pKa = 11.84EE23 pKa = 4.44LIAEE27 pKa = 4.29EE28 pKa = 4.47GNPDD32 pKa = 2.99LKK34 pKa = 11.14LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.97GFTFDD54 pKa = 3.91EE55 pKa = 5.11LVNEE59 pKa = 5.34DD60 pKa = 3.49DD61 pKa = 3.72TQIARR66 pKa = 11.84EE67 pKa = 4.19GVTFLVDD74 pKa = 3.53PMSYY78 pKa = 10.19QYY80 pKa = 11.57LVGAEE85 pKa = 3.69IDD87 pKa = 3.85YY88 pKa = 11.37QEE90 pKa = 4.56GLEE93 pKa = 4.22GSQFVIKK100 pKa = 10.58NPNATTTCGCGSSFSVV116 pKa = 3.54

Molecular weight: 12.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R7B266|A0A4R7B266_9NEIS Glycosyl transferase family 2 OS=Paludibacterium purpuratum OX=1144873 GN=DFP86_10958 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.31QPSTTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.24RR14 pKa = 11.84THH16 pKa = 5.92GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3774

0

3774

1213726

29

3726

321.6

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.693 ± 0.053

1.088 ± 0.017

5.465 ± 0.033

5.164 ± 0.036

3.582 ± 0.027

7.869 ± 0.038

2.42 ± 0.017

4.83 ± 0.032

3.18 ± 0.036

11.551 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.019

2.917 ± 0.029

4.895 ± 0.032

4.465 ± 0.037

6.662 ± 0.038

5.682 ± 0.032

4.978 ± 0.033

7.082 ± 0.035

1.433 ± 0.018

2.479 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski