Pusillimonas ginsengisoli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pusillimonas

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4096 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U5LFT4|A0A4U5LFT4_9BURK Uncharacterized protein OS=Pusillimonas ginsengisoli OX=453575 GN=D7I39_15510 PE=4 SV=1
MM1 pKa = 6.89TRR3 pKa = 11.84SLIALALISSFAAVGTANATASTTTANFNVTINVLAGCEE42 pKa = 3.99FTTDD46 pKa = 3.62GGISDD51 pKa = 4.55LAFGSVAATPLTGTAPVSTAQTSFDD76 pKa = 3.84LQCTVGTAPEE86 pKa = 3.86IALTSAHH93 pKa = 5.21EE94 pKa = 4.15WEE96 pKa = 4.91MEE98 pKa = 4.33GSDD101 pKa = 5.01ADD103 pKa = 3.78NTEE106 pKa = 3.78ATISYY111 pKa = 10.89ALYY114 pKa = 10.22SDD116 pKa = 4.24SARR119 pKa = 11.84TTAWGDD125 pKa = 3.2TATITGADD133 pKa = 3.08TGAAQTHH140 pKa = 4.57VVYY143 pKa = 10.54GKK145 pKa = 9.45VANPGRR151 pKa = 11.84VAGNFKK157 pKa = 10.96DD158 pKa = 3.9VVTVTLTYY166 pKa = 11.12

Molecular weight: 16.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U5LLM1|A0A4U5LLM1_9BURK Transporter substrate-binding domain-containing protein OS=Pusillimonas ginsengisoli OX=453575 GN=D7I39_04240 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.95TRR25 pKa = 11.84SGRR28 pKa = 11.84AIINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.03QLAVV44 pKa = 3.26

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4096

0

4096

1320091

24

3017

322.3

35.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.272 ± 0.044

0.965 ± 0.012

5.282 ± 0.026

5.154 ± 0.035

3.621 ± 0.023

8.102 ± 0.043

2.34 ± 0.019

5.303 ± 0.03

3.451 ± 0.03

10.583 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.669 ± 0.021

3.012 ± 0.025

4.876 ± 0.027

4.089 ± 0.025

6.239 ± 0.036

6.104 ± 0.034

5.483 ± 0.029

7.491 ± 0.032

1.426 ± 0.018

2.539 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski