Polaribacter reichenbachii

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3418 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1B8U4M2|A0A1B8U4M2_9FLAO Peptidase_S8 domain-containing protein OS=Polaribacter reichenbachii OX=996801 GN=LPB301_05110 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.44SKK4 pKa = 10.85LSVFIFILFIFYY16 pKa = 10.82CKK18 pKa = 10.0DD19 pKa = 3.06VKK21 pKa = 11.06AQLSKK26 pKa = 11.05KK27 pKa = 10.04HH28 pKa = 6.45FIPPLTYY35 pKa = 10.53AEE37 pKa = 4.47EE38 pKa = 4.67GNANPEE44 pKa = 3.76SQYY47 pKa = 11.07FYY49 pKa = 10.92ISTPTNKK56 pKa = 9.95DD57 pKa = 2.52VSYY60 pKa = 9.75TIKK63 pKa = 10.78QIGQDD68 pKa = 3.32NDD70 pKa = 2.84ITGIVSSSNPQEE82 pKa = 3.95IFIATGDD89 pKa = 3.59SQLFVNANEE98 pKa = 4.26TGTVHH103 pKa = 5.92TNKK106 pKa = 10.34GYY108 pKa = 10.12IIEE111 pKa = 4.52ATDD114 pKa = 3.52VIYY117 pKa = 11.25VSVRR121 pKa = 11.84VLAGGGAQAGALVSKK136 pKa = 10.56GSSALGTTFRR146 pKa = 11.84AGMFTNEE153 pKa = 4.17NPQSNYY159 pKa = 10.6LNFVSVMASEE169 pKa = 5.52DD170 pKa = 3.41NTQITFDD177 pKa = 5.46DD178 pKa = 3.76ITPGLEE184 pKa = 3.57IKK186 pKa = 10.55NYY188 pKa = 9.26TSTFPFSVVLNEE200 pKa = 3.97GEE202 pKa = 4.38SYY204 pKa = 10.54IVATSADD211 pKa = 3.81DD212 pKa = 4.22VIVNRR217 pKa = 11.84DD218 pKa = 3.44GLIGTLISSDD228 pKa = 3.16KK229 pKa = 10.99NIVVNTGSANGSFHH243 pKa = 6.99NGGGRR248 pKa = 11.84DD249 pKa = 3.61YY250 pKa = 11.43GIDD253 pKa = 3.54QIVGADD259 pKa = 4.08KK260 pKa = 10.85IGTDD264 pKa = 3.82YY265 pKa = 11.29IFVKK269 pKa = 10.85GDD271 pKa = 3.51GSNGWEE277 pKa = 3.82NVLIVAHH284 pKa = 6.89EE285 pKa = 4.71DD286 pKa = 3.22NTVVSRR292 pKa = 11.84NGINSTINKK301 pKa = 9.71GEE303 pKa = 3.95YY304 pKa = 9.34TLIEE308 pKa = 4.2GDD310 pKa = 3.58EE311 pKa = 4.37FNANGNLFVKK321 pKa = 9.82TSKK324 pKa = 10.21PAFAYY329 pKa = 10.23QGVGANTSEE338 pKa = 4.34ANQGMFFVPPLSCEE352 pKa = 4.01SRR354 pKa = 11.84GKK356 pKa = 10.39VDD358 pKa = 4.6NIPNIEE364 pKa = 4.48SIGNTTFTGGITIVTNVNASVNINSQPITNFATSGPFSIDD404 pKa = 2.8IDD406 pKa = 3.75NDD408 pKa = 4.19GIADD412 pKa = 3.77YY413 pKa = 8.03EE414 pKa = 4.66TYY416 pKa = 10.77KK417 pKa = 9.7VTNLTGNISVDD428 pKa = 3.42SSDD431 pKa = 4.26EE432 pKa = 4.18LYY434 pKa = 10.82CAYY437 pKa = 10.75FNVNGAATSGSFYY450 pKa = 10.75SGFPTAPEE458 pKa = 4.06INFDD462 pKa = 3.93TTVSTLGNCIPNLTLEE478 pKa = 4.46AANTNLFDD486 pKa = 4.58SIKK489 pKa = 9.26WEE491 pKa = 4.32YY492 pKa = 10.66YY493 pKa = 10.79NEE495 pKa = 4.25TTSTWEE501 pKa = 3.84EE502 pKa = 3.48RR503 pKa = 11.84SSNNTYY509 pKa = 10.71KK510 pKa = 10.63PIEE513 pKa = 4.37SEE515 pKa = 3.69PGKK518 pKa = 10.63YY519 pKa = 9.73RR520 pKa = 11.84LIGTIDD526 pKa = 3.35CTGATFEE533 pKa = 4.72SIEE536 pKa = 4.36IPVSICPDD544 pKa = 3.77DD545 pKa = 4.31FDD547 pKa = 6.96GDD549 pKa = 4.63LIIDD553 pKa = 4.18NLDD556 pKa = 3.02VDD558 pKa = 4.21IDD560 pKa = 3.72NDD562 pKa = 4.77GILNCDD568 pKa = 3.39EE569 pKa = 4.47SLGNATLNLADD580 pKa = 4.8ADD582 pKa = 3.78NPEE585 pKa = 5.31IIFQDD590 pKa = 3.26SSTNSAILSRR600 pKa = 11.84VYY602 pKa = 10.8SEE604 pKa = 4.89SDD606 pKa = 3.17VTNTYY611 pKa = 9.23TGQNNGNFQSIIYY624 pKa = 9.21PSVDD628 pKa = 2.7SKK630 pKa = 11.46LQYY633 pKa = 10.44EE634 pKa = 4.64LNFTQNVNFKK644 pKa = 8.89FTQNRR649 pKa = 11.84NLDD652 pKa = 3.53HH653 pKa = 6.94TISDD657 pKa = 3.98GEE659 pKa = 4.14FFILKK664 pKa = 10.04IGPSNKK670 pKa = 9.51NVTLLDD676 pKa = 4.56PDD678 pKa = 4.11DD679 pKa = 4.01QLLIDD684 pKa = 4.45SNFDD688 pKa = 3.46GEE690 pKa = 4.52FEE692 pKa = 4.32SGITNISASEE702 pKa = 3.48IHH704 pKa = 6.63FKK706 pKa = 11.17YY707 pKa = 10.25KK708 pKa = 10.99ANTTGASSNFKK719 pKa = 10.26FVANQVDD726 pKa = 3.9QIDD729 pKa = 4.42FKK731 pKa = 11.29HH732 pKa = 6.07QSTGLAASSTFSGNLEE748 pKa = 4.19LTCFTLDD755 pKa = 3.25SDD757 pKa = 4.36GDD759 pKa = 4.15GIEE762 pKa = 4.59NMFDD766 pKa = 3.83LDD768 pKa = 4.16SDD770 pKa = 3.87NDD772 pKa = 4.8GIPDD776 pKa = 3.4ISEE779 pKa = 3.91SSATKK784 pKa = 10.21IILSNTDD791 pKa = 3.0ADD793 pKa = 5.33LDD795 pKa = 3.92GLDD798 pKa = 4.53DD799 pKa = 3.93VFIGIITNIDD809 pKa = 3.4TDD811 pKa = 3.58NDD813 pKa = 4.82GIPNHH818 pKa = 6.95LDD820 pKa = 2.75VDD822 pKa = 4.04ADD824 pKa = 3.52NDD826 pKa = 4.82GIFDD830 pKa = 3.89FVEE833 pKa = 4.17AGITEE838 pKa = 4.16TDD840 pKa = 3.41ANNDD844 pKa = 4.17GILDD848 pKa = 4.19DD849 pKa = 4.13ATTANVGLNGLLNALEE865 pKa = 4.22TSPDD869 pKa = 3.73NNILAYY875 pKa = 9.65TILDD879 pKa = 3.5TDD881 pKa = 4.1ADD883 pKa = 3.94NIYY886 pKa = 11.24NFLEE890 pKa = 4.31LDD892 pKa = 3.94ADD894 pKa = 3.83NDD896 pKa = 3.5NCFDD900 pKa = 3.57VFEE903 pKa = 6.32AGFTDD908 pKa = 4.12NNDD911 pKa = 3.86DD912 pKa = 4.9GILDD916 pKa = 3.73ATPFSVNNNGKK927 pKa = 9.94IINNSDD933 pKa = 3.49GYY935 pKa = 6.96TTPNSDD941 pKa = 4.72YY942 pKa = 9.7ITSAPIEE949 pKa = 4.27INTPFEE955 pKa = 4.41DD956 pKa = 5.37VIFCEE961 pKa = 4.06NDD963 pKa = 2.86TDD965 pKa = 4.84VITIDD970 pKa = 3.3TTADD974 pKa = 3.13SFQWQISPDD983 pKa = 3.31GSTWSSITDD992 pKa = 3.19NAVYY996 pKa = 10.75SNSTSASLQITNTPLSYY1013 pKa = 10.69HH1014 pKa = 6.45NYY1016 pKa = 7.99QYY1018 pKa = 11.21RR1019 pKa = 11.84VILNRR1024 pKa = 11.84TGNACTEE1031 pKa = 4.19TSNSITLTVNPLPILKK1047 pKa = 10.33ANPEE1051 pKa = 4.31LNQCIEE1057 pKa = 4.15VADD1060 pKa = 4.14TNTTVNLTLAEE1071 pKa = 4.21INISEE1076 pKa = 4.45TTNVTFEE1083 pKa = 4.46YY1084 pKa = 10.4YY1085 pKa = 10.75EE1086 pKa = 4.46DD1087 pKa = 3.45MAGANLIADD1096 pKa = 3.85PTSYY1100 pKa = 10.29PVQVNITEE1108 pKa = 4.25TVYY1111 pKa = 11.2VKK1113 pKa = 10.69VISEE1117 pKa = 4.3FSCEE1121 pKa = 3.89TDD1123 pKa = 4.02LSEE1126 pKa = 4.16LTINVGQTIRR1136 pKa = 11.84NSYY1139 pKa = 11.08NDD1141 pKa = 4.28LQPPVCDD1148 pKa = 4.84DD1149 pKa = 3.9FLDD1152 pKa = 3.9VDD1154 pKa = 4.34GNDD1157 pKa = 3.37TAVNSDD1163 pKa = 3.38TDD1165 pKa = 3.98NITNFYY1171 pKa = 10.25LDD1173 pKa = 3.22KK1174 pKa = 10.43TAIINGINPPMNTEE1188 pKa = 3.7VFFYY1192 pKa = 10.61EE1193 pKa = 4.47NEE1195 pKa = 4.0SDD1197 pKa = 4.37RR1198 pKa = 11.84DD1199 pKa = 3.6NSLNEE1204 pKa = 3.52IDD1206 pKa = 3.64VTNFRR1211 pKa = 11.84NNISKK1216 pKa = 10.81NEE1218 pKa = 4.09VINIPNGIQFPIFYY1232 pKa = 10.01KK1233 pKa = 10.42ILSTINNDD1241 pKa = 3.36CQGIGEE1247 pKa = 4.85FYY1249 pKa = 10.84VQIDD1253 pKa = 4.01TVPTAEE1259 pKa = 4.19VVPDD1263 pKa = 5.27LEE1265 pKa = 5.18LCDD1268 pKa = 5.09DD1269 pKa = 5.4AIDD1272 pKa = 4.19GNATNGIVQYY1282 pKa = 11.0FDD1284 pKa = 5.42LEE1286 pKa = 4.47SQTTAILNGQNAADD1300 pKa = 3.87FTVTYY1305 pKa = 10.2HH1306 pKa = 7.98DD1307 pKa = 4.73SEE1309 pKa = 4.4TDD1311 pKa = 3.15ANAGIDD1317 pKa = 3.71EE1318 pKa = 4.34LTSPFEE1324 pKa = 3.78NSVRR1328 pKa = 11.84DD1329 pKa = 3.63SQPIYY1334 pKa = 11.18VRR1336 pKa = 11.84VTNNTTGCFTDD1347 pKa = 3.2HH1348 pKa = 6.83TSFNVIVNPVPIANFVNDD1366 pKa = 5.67LEE1368 pKa = 4.59VCDD1371 pKa = 5.04DD1372 pKa = 4.13NSDD1375 pKa = 3.19GSARR1379 pKa = 11.84NGFSQSIDD1387 pKa = 3.94LEE1389 pKa = 4.39SQTAGILGAQDD1400 pKa = 3.75PNIHH1404 pKa = 6.17IVTYY1408 pKa = 10.27HH1409 pKa = 7.04RR1410 pKa = 11.84SLADD1414 pKa = 3.47AQSGNLPLVSPYY1426 pKa = 10.63TNNTIDD1432 pKa = 3.64RR1433 pKa = 11.84EE1434 pKa = 4.41TIYY1437 pKa = 11.16VRR1439 pKa = 11.84ILNTNSMCVNGISNFDD1455 pKa = 3.86VIVNPEE1461 pKa = 4.09PTFEE1465 pKa = 4.35TPTNLAYY1472 pKa = 10.77CDD1474 pKa = 4.77DD1475 pKa = 5.16DD1476 pKa = 6.7LDD1478 pKa = 6.17GDD1480 pKa = 4.13DD1481 pKa = 5.54ANGIVQNIDD1490 pKa = 3.41LDD1492 pKa = 4.27SKK1494 pKa = 9.78ITEE1497 pKa = 4.09ILGASQSPNDD1507 pKa = 3.63FWVTFHH1513 pKa = 7.59KK1514 pKa = 10.75SQADD1518 pKa = 3.5ATSGNDD1524 pKa = 3.51PVVSPYY1530 pKa = 11.15QNTT1533 pKa = 3.29

Molecular weight:
166.7 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1B8TWH8|A0A1B8TWH8_9FLAO Malate dehydrogenase OS=Polaribacter reichenbachii OX=996801 GN=mdh PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 3.92TITTDD27 pKa = 3.73KK28 pKa = 10.91PEE30 pKa = 4.37KK31 pKa = 10.55SLLAPKK37 pKa = 9.91KK38 pKa = 10.38RR39 pKa = 11.84SGGRR43 pKa = 11.84NNQGRR48 pKa = 11.84MTTRR52 pKa = 11.84NIGGGHH58 pKa = 4.99KK59 pKa = 9.43QRR61 pKa = 11.84YY62 pKa = 8.74RR63 pKa = 11.84IIDD66 pKa = 3.84FKK68 pKa = 10.95RR69 pKa = 11.84DD70 pKa = 3.68KK71 pKa = 11.21NDD73 pKa = 2.9MPATVKK79 pKa = 10.14TIEE82 pKa = 4.0YY83 pKa = 9.86DD84 pKa = 3.47PNRR87 pKa = 11.84TAFIALLSYY96 pKa = 11.49ADD98 pKa = 3.5GEE100 pKa = 4.22KK101 pKa = 10.25RR102 pKa = 11.84YY103 pKa = 10.74VIAQNGLKK111 pKa = 10.19VGQTIVSGSGVSPEE125 pKa = 4.69IGNAMPLSEE134 pKa = 4.97IPLGTTISCIEE145 pKa = 3.88LHH147 pKa = 6.66PGQGAVMARR156 pKa = 11.84SAGSFAQLMARR167 pKa = 11.84DD168 pKa = 3.96GKK170 pKa = 10.52YY171 pKa = 9.12ATVKK175 pKa = 10.42LPSGEE180 pKa = 4.06TRR182 pKa = 11.84LILLTCSATIGVVSNSDD199 pKa = 3.21HH200 pKa = 6.09QLLVSGKK207 pKa = 8.88AGRR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84VNAVRR224 pKa = 11.84MNPVDD229 pKa = 3.4HH230 pKa = 7.07PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.41GFKK256 pKa = 9.24TRR258 pKa = 11.84SKK260 pKa = 9.37TKK262 pKa = 10.4ASNKK266 pKa = 9.95YY267 pKa = 9.56IIEE270 pKa = 4.06RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.1KK274 pKa = 9.82

Molecular weight:
29.88 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3418

0

3418

1227233

50

2383

359.1

40.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.955 ± 0.046

0.656 ± 0.011

5.61 ± 0.029

6.479 ± 0.041

5.46 ± 0.041

6.142 ± 0.044

1.624 ± 0.018

8.363 ± 0.04

8.775 ± 0.056

9.038 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.948 ± 0.019

6.952 ± 0.051

3.13 ± 0.02

3.156 ± 0.022

3.085 ± 0.022

6.57 ± 0.033

5.857 ± 0.034

5.967 ± 0.027

1.115 ± 0.016

4.12 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski