Bovine ephemeral fever virus (BEFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ephemerovirus; Bovine fever ephemerovirus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0V8W3|A0A0A0V8W3_BEFV GDP polyribonucleotidyltransferase OS=Bovine ephemeral fever virus OX=11303 GN=L PE=4 SV=1
MM1 pKa = 7.31SHH3 pKa = 7.47LKK5 pKa = 9.92PKK7 pKa = 10.41IMTGAYY13 pKa = 9.75DD14 pKa = 3.76AEE16 pKa = 4.35KK17 pKa = 10.59LRR19 pKa = 11.84KK20 pKa = 9.14NLQEE24 pKa = 4.04QIALEE29 pKa = 4.09EE30 pKa = 4.8DD31 pKa = 3.4EE32 pKa = 6.26LEE34 pKa = 4.34NQTDD38 pKa = 3.97FEE40 pKa = 4.85NNKK43 pKa = 9.94EE44 pKa = 3.97EE45 pKa = 4.88SNISNNPLIIKK56 pKa = 8.35QEE58 pKa = 3.87PGIYY62 pKa = 9.52PIEE65 pKa = 4.63INLEE69 pKa = 4.08DD70 pKa = 4.0LEE72 pKa = 4.34KK73 pKa = 11.29ANGIEE78 pKa = 4.02EE79 pKa = 4.12DD80 pKa = 3.85WEE82 pKa = 4.14NSLLKK87 pKa = 10.15IIEE90 pKa = 4.46SSDD93 pKa = 3.47VTPKK97 pKa = 10.86LSWDD101 pKa = 3.87DD102 pKa = 3.58EE103 pKa = 4.76FEE105 pKa = 4.27NDD107 pKa = 3.52TYY109 pKa = 11.17KK110 pKa = 11.06GCNVFTKK117 pKa = 10.25DD118 pKa = 3.82LCNDD122 pKa = 3.38SGNQEE127 pKa = 4.11KK128 pKa = 11.23NNVQIKK134 pKa = 9.72QSSLADD140 pKa = 3.51VAQVLSLFHH149 pKa = 6.48IRR151 pKa = 11.84SDD153 pKa = 3.1VDD155 pKa = 3.55YY156 pKa = 9.08KK157 pKa = 10.23TEE159 pKa = 4.01KK160 pKa = 10.43DD161 pKa = 3.35NKK163 pKa = 9.64GLVRR167 pKa = 11.84IIKK170 pKa = 10.09LSKK173 pKa = 9.22KK174 pKa = 10.33DD175 pKa = 3.8KK176 pKa = 10.76SIEE179 pKa = 4.19SNKK182 pKa = 9.84RR183 pKa = 11.84DD184 pKa = 3.51IVDD187 pKa = 3.55QEE189 pKa = 4.31SDD191 pKa = 2.9KK192 pKa = 10.89HH193 pKa = 5.39TEE195 pKa = 3.99CNSNFDD201 pKa = 3.5AVMDD205 pKa = 3.71QFQKK209 pKa = 11.01GIRR212 pKa = 11.84VKK214 pKa = 10.61KK215 pKa = 10.24RR216 pKa = 11.84FGKK219 pKa = 10.76GYY221 pKa = 10.97VKK223 pKa = 10.44INADD227 pKa = 3.65NMPGTYY233 pKa = 9.53HH234 pKa = 7.52DD235 pKa = 5.06LYY237 pKa = 11.47NVISAVKK244 pKa = 9.83GEE246 pKa = 4.14KK247 pKa = 9.12TVEE250 pKa = 3.53EE251 pKa = 4.31MIRR254 pKa = 11.84YY255 pKa = 8.27LFKK258 pKa = 10.55KK259 pKa = 10.03SKK261 pKa = 9.79SFKK264 pKa = 10.62SISKK268 pKa = 8.5TLNIDD273 pKa = 3.89EE274 pKa = 5.4MILCC278 pKa = 4.91

Molecular weight: 32.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0VBA2|A0A0A0VBA2_BEFV Isoform of A0A0A0V4V7 Alpha 3 protein OS=Bovine ephemeral fever virus OX=11303 GN=alpha PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 6.12KK3 pKa = 10.88GLLSNFWNDD12 pKa = 2.85FKK14 pKa = 11.19QWSEE18 pKa = 3.81DD19 pKa = 3.21RR20 pKa = 11.84KK21 pKa = 10.5VEE23 pKa = 4.54IVNWWSNLEE32 pKa = 4.17SKK34 pKa = 10.51VRR36 pKa = 11.84LGFWIILIILLGILAIRR53 pKa = 11.84IAIKK57 pKa = 10.34VYY59 pKa = 10.12QCVKK63 pKa = 9.78LTTKK67 pKa = 10.14GVKK70 pKa = 9.62KK71 pKa = 10.09IKK73 pKa = 10.25RR74 pKa = 11.84VIKK77 pKa = 10.08KK78 pKa = 9.4KK79 pKa = 10.62RR80 pKa = 11.84SIKK83 pKa = 10.22KK84 pKa = 9.1YY85 pKa = 10.38RR86 pKa = 11.84KK87 pKa = 6.76TT88 pKa = 3.33

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

3

12

4809

48

2144

400.8

46.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.807 ± 0.482

1.975 ± 0.22

5.614 ± 0.369

7.798 ± 0.419

4.034 ± 0.203

5.511 ± 0.312

2.371 ± 0.227

8.526 ± 0.435

8.692 ± 0.519

10.002 ± 0.79

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.343

6.758 ± 0.264

3.098 ± 0.224

2.89 ± 0.15

5.053 ± 0.324

7.216 ± 0.515

4.408 ± 0.328

4.658 ± 0.396

1.871 ± 0.209

4.055 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski