Buchananella hordeovulneris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; <

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5PV45|A0A1Q5PV45_9ACTO DUF4235 domain-containing protein OS=Buchananella hordeovulneris OX=52770 GN=BSZ40_07250 PE=4 SV=1
MM1 pKa = 7.63SNYY4 pKa = 10.16QMDD7 pKa = 4.29EE8 pKa = 4.05FDD10 pKa = 5.11HH11 pKa = 7.96VSDD14 pKa = 4.31DD15 pKa = 3.71QPEE18 pKa = 4.55GVHH21 pKa = 6.55RR22 pKa = 11.84APEE25 pKa = 4.23PWWRR29 pKa = 11.84PLLPYY34 pKa = 10.21LAVIILIPLLAWGAVAMLSKK54 pKa = 10.97SGDD57 pKa = 3.38QPTAGGGAAGVQTAPAADD75 pKa = 4.44DD76 pKa = 4.27GASQSVPAPATEE88 pKa = 3.83QATAPEE94 pKa = 4.1QAPATTAPAPPPEE107 pKa = 4.19QTPTPSAPEE116 pKa = 3.89GVNYY120 pKa = 9.61EE121 pKa = 4.27SQVAVYY127 pKa = 9.59NAAGIQGMAAAAVEE141 pKa = 4.76TIAEE145 pKa = 4.15AGFANGSATNFDD157 pKa = 4.07KK158 pKa = 10.93EE159 pKa = 4.43SPEE162 pKa = 4.1ANTVYY167 pKa = 10.55YY168 pKa = 10.87ASAEE172 pKa = 4.12HH173 pKa = 6.69AAAAQEE179 pKa = 4.0IATALGIGNVVEE191 pKa = 4.53DD192 pKa = 4.28ASIASSTPIAVVLVEE207 pKa = 4.47DD208 pKa = 4.83FRR210 pKa = 6.51

Molecular weight: 21.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5PUM4|A0A1Q5PUM4_9ACTO Sugar ABC transporter permease OS=Buchananella hordeovulneris OX=52770 GN=BSZ40_08125 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 9.27RR13 pKa = 11.84SKK15 pKa = 10.08VHH17 pKa = 6.29GFRR20 pKa = 11.84QRR22 pKa = 11.84MSSRR26 pKa = 11.84AGRR29 pKa = 11.84AILAMRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.85GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2109

0

2109

729133

32

2229

345.7

37.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.324 ± 0.085

0.738 ± 0.014

5.312 ± 0.038

5.53 ± 0.055

2.897 ± 0.032

8.577 ± 0.047

2.152 ± 0.023

3.615 ± 0.041

2.146 ± 0.039

10.9 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.611 ± 0.02

1.957 ± 0.028

5.932 ± 0.059

3.863 ± 0.033

7.151 ± 0.054

4.973 ± 0.035

6.052 ± 0.04

8.7 ± 0.049

1.569 ± 0.025

2.001 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski