Wenzhou weivirus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KL43|A0A1L3KL43_9VIRU RNA-dependent RNA polymerase OS=Wenzhou weivirus-like virus 1 OX=1923682 PE=4 SV=1
MM1 pKa = 7.36LSVRR5 pKa = 11.84EE6 pKa = 4.23GFSDD10 pKa = 3.92LKK12 pKa = 10.33YY13 pKa = 11.25GEE15 pKa = 4.17VAPYY19 pKa = 10.24LRR21 pKa = 11.84EE22 pKa = 3.83RR23 pKa = 11.84AAKK26 pKa = 9.99RR27 pKa = 11.84QGEE30 pKa = 4.27WTLTVHH36 pKa = 6.0EE37 pKa = 5.67AEE39 pKa = 4.93DD40 pKa = 3.44SWLGILSRR48 pKa = 11.84AVSFKK53 pKa = 10.98VVLTEE58 pKa = 3.73EE59 pKa = 4.88EE60 pKa = 4.31YY61 pKa = 11.4AKK63 pKa = 10.67LSRR66 pKa = 11.84ALYY69 pKa = 9.61HH70 pKa = 7.03GIRR73 pKa = 11.84NGTKK77 pKa = 8.8KK78 pKa = 9.31TSLLANLEE86 pKa = 4.03NTAQALWPTGSLDD99 pKa = 3.55HH100 pKa = 6.81VAMRR104 pKa = 11.84HH105 pKa = 4.8LAVTAVVQYY114 pKa = 7.36VTKK117 pKa = 10.44EE118 pKa = 3.77STPQGFRR125 pKa = 11.84LPYY128 pKa = 8.31WMTWGAMVTQATVGVHH144 pKa = 4.45ILGRR148 pKa = 11.84LVKK151 pKa = 10.35GAARR155 pKa = 11.84RR156 pKa = 11.84AWITGPHH163 pKa = 6.99PGPFGQLIMRR173 pKa = 11.84EE174 pKa = 4.28YY175 pKa = 10.32FATPALVMWALMTVISYY192 pKa = 10.52CGARR196 pKa = 11.84GITLSWRR203 pKa = 11.84SVLPVLSRR211 pKa = 11.84NAGTTRR217 pKa = 11.84TGPAAEE223 pKa = 4.72AAPAAPGGPPPAGATPGAGGAAPGPAGGAPPSGGTPAAGAPAPPSPPVMEE273 pKa = 4.43QPATPEE279 pKa = 4.12DD280 pKa = 4.01PEE282 pKa = 4.09NAANASEE289 pKa = 4.39DD290 pKa = 3.81LRR292 pKa = 11.84TMEE295 pKa = 4.38MPGKK299 pKa = 9.9IVGVLGQNFDD309 pKa = 3.24KK310 pKa = 10.92DD311 pKa = 3.91LPVNHH316 pKa = 6.78LPVVGVLVGPCQRR329 pKa = 11.84KK330 pKa = 8.28PNVYY334 pKa = 10.3SKK336 pKa = 9.71TVEE339 pKa = 4.0NLKK342 pKa = 10.56AAINEE347 pKa = 4.26RR348 pKa = 11.84IIKK351 pKa = 9.84KK352 pKa = 9.6ARR354 pKa = 11.84KK355 pKa = 8.64PKK357 pKa = 9.95LCRR360 pKa = 11.84ADD362 pKa = 3.37RR363 pKa = 11.84VRR365 pKa = 11.84ISRR368 pKa = 11.84LVRR371 pKa = 11.84KK372 pKa = 9.96AMSNSEE378 pKa = 3.84KK379 pKa = 10.61HH380 pKa = 6.06GVFSKK385 pKa = 10.94SRR387 pKa = 11.84IQEE390 pKa = 3.46WAIQHH395 pKa = 5.96FNLEE399 pKa = 4.26EE400 pKa = 4.01MKK402 pKa = 10.26SQKK405 pKa = 9.45WSLEE409 pKa = 3.81RR410 pKa = 11.84FRR412 pKa = 11.84NALKK416 pKa = 10.58NLYY419 pKa = 9.78EE420 pKa = 4.65KK421 pKa = 11.14DD422 pKa = 3.47PVEE425 pKa = 4.51FMLKK429 pKa = 10.47AGIKK433 pKa = 8.23PEE435 pKa = 3.94CMEE438 pKa = 4.21EE439 pKa = 4.29GKK441 pKa = 10.61APRR444 pKa = 11.84MLIADD449 pKa = 3.91GDD451 pKa = 4.11EE452 pKa = 4.41GQLMALAVIKK462 pKa = 10.61CFEE465 pKa = 4.11EE466 pKa = 4.39LLFKK470 pKa = 10.82HH471 pKa = 6.16FEE473 pKa = 3.99EE474 pKa = 5.51RR475 pKa = 11.84SIKK478 pKa = 10.33HH479 pKa = 5.45LSKK482 pKa = 10.65HH483 pKa = 4.81EE484 pKa = 3.85AMEE487 pKa = 4.0RR488 pKa = 11.84VVRR491 pKa = 11.84VLKK494 pKa = 10.84KK495 pKa = 9.43KK496 pKa = 9.74GARR499 pKa = 11.84AVEE502 pKa = 4.36GDD504 pKa = 3.23GSAWDD509 pKa = 3.99TTCGVLVRR517 pKa = 11.84SLIEE521 pKa = 3.88NPILFHH527 pKa = 7.03IFRR530 pKa = 11.84TLAEE534 pKa = 4.3FGVIPEE540 pKa = 3.86TWMAEE545 pKa = 3.64HH546 pKa = 6.86HH547 pKa = 6.15AACTKK552 pKa = 10.58EE553 pKa = 3.72KK554 pKa = 10.95LKK556 pKa = 11.22LFFSNKK562 pKa = 6.27FQKK565 pKa = 9.2VTVTIDD571 pKa = 3.78AIRR574 pKa = 11.84RR575 pKa = 11.84SGHH578 pKa = 6.55RR579 pKa = 11.84GTSCLNWWINFTMWVSSIFKK599 pKa = 9.87EE600 pKa = 4.25PEE602 pKa = 3.66RR603 pKa = 11.84FLDD606 pKa = 3.49PDD608 pKa = 3.5VRR610 pKa = 11.84KK611 pKa = 8.86GTDD614 pKa = 3.13LTEE617 pKa = 4.62KK618 pKa = 9.66EE619 pKa = 4.24RR620 pKa = 11.84WWNGTFEE627 pKa = 5.69GDD629 pKa = 4.45DD630 pKa = 4.39SLCTMCPPMLKK641 pKa = 9.7DD642 pKa = 4.93DD643 pKa = 3.87KK644 pKa = 10.92MSDD647 pKa = 3.23IFCQFWSDD655 pKa = 2.97AGFNMKK661 pKa = 9.61IVYY664 pKa = 9.59CDD666 pKa = 3.13RR667 pKa = 11.84RR668 pKa = 11.84ATFTGWHH675 pKa = 5.94LVCEE679 pKa = 4.38NGEE682 pKa = 4.21LTDD685 pKa = 3.4VRR687 pKa = 11.84APEE690 pKa = 4.31LPRR693 pKa = 11.84ALANSGVSVSPSAVQAAKK711 pKa = 10.16DD712 pKa = 3.79GKK714 pKa = 9.2MSAIKK719 pKa = 10.46VIAAASALARR729 pKa = 11.84ASDD732 pKa = 3.83FSGILPTVSNKK743 pKa = 9.53YY744 pKa = 9.31LQYY747 pKa = 11.45ANEE750 pKa = 4.35CSKK753 pKa = 11.18SDD755 pKa = 3.71YY756 pKa = 10.85VDD758 pKa = 3.73RR759 pKa = 11.84EE760 pKa = 3.81CSIRR764 pKa = 11.84CFGFDD769 pKa = 2.94GHH771 pKa = 5.79SAKK774 pKa = 10.05EE775 pKa = 3.68VRR777 pKa = 11.84EE778 pKa = 4.15RR779 pKa = 11.84IEE781 pKa = 3.78EE782 pKa = 4.07RR783 pKa = 11.84NLGVTHH789 pKa = 6.82QDD791 pKa = 3.13EE792 pKa = 4.64QKK794 pKa = 8.66TLEE797 pKa = 4.02MLGFKK802 pKa = 9.06ATHH805 pKa = 6.97DD806 pKa = 3.58EE807 pKa = 4.4LMTFTEE813 pKa = 5.29HH814 pKa = 7.16IWDD817 pKa = 4.77LEE819 pKa = 4.09PATLTAYY826 pKa = 10.05QAFRR830 pKa = 11.84EE831 pKa = 4.25SLPASWRR838 pKa = 11.84MEE840 pKa = 3.85

Molecular weight: 93.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KL43|A0A1L3KL43_9VIRU RNA-dependent RNA polymerase OS=Wenzhou weivirus-like virus 1 OX=1923682 PE=4 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84GGRR6 pKa = 11.84RR7 pKa = 11.84AGRR10 pKa = 11.84AKK12 pKa = 10.42AQPKK16 pKa = 9.39PEE18 pKa = 4.19KK19 pKa = 9.8KK20 pKa = 10.32AQGGQTSAPRR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 9.64RR33 pKa = 11.84RR34 pKa = 11.84GRR36 pKa = 11.84PNSGWAVNGRR46 pKa = 11.84AHH48 pKa = 6.81HH49 pKa = 5.45GHH51 pKa = 6.45YY52 pKa = 10.62DD53 pKa = 3.15AFDD56 pKa = 3.77PRR58 pKa = 11.84KK59 pKa = 9.78QPMARR64 pKa = 11.84AVGHH68 pKa = 5.43ATAVRR73 pKa = 11.84GFGRR77 pKa = 11.84LKK79 pKa = 10.49RR80 pKa = 11.84PSFNDD85 pKa = 2.79GMEE88 pKa = 3.9QMYY91 pKa = 10.7VFMPSTTRR99 pKa = 11.84VVGLYY104 pKa = 10.81GNYY107 pKa = 10.04KK108 pKa = 10.32SDD110 pKa = 3.07IDD112 pKa = 3.49QWRR115 pKa = 11.84GAGPNVDD122 pKa = 3.56TNTALIEE129 pKa = 3.98NMGFSEE135 pKa = 4.86EE136 pKa = 4.4SGPHH140 pKa = 3.81QTLLGKK146 pKa = 10.32FSVRR150 pKa = 11.84LRR152 pKa = 11.84NISKK156 pKa = 10.8AMDD159 pKa = 3.64ASGSVYY165 pKa = 10.66VLSLNAGMFLEE176 pKa = 4.63SLNGDD181 pKa = 2.99WTARR185 pKa = 11.84TNWTRR190 pKa = 11.84LRR192 pKa = 11.84NYY194 pKa = 10.19VLGSPRR200 pKa = 11.84TRR202 pKa = 11.84TFSGSEE208 pKa = 3.87LLKK211 pKa = 8.78TRR213 pKa = 11.84QWNTHH218 pKa = 6.75PIDD221 pKa = 3.49ATRR224 pKa = 11.84SQEE227 pKa = 4.04FQMVPPATPDD237 pKa = 3.41TQVTTFRR244 pKa = 11.84NSMDD248 pKa = 3.81SPTHH252 pKa = 6.51SMVVFLFIPGEE263 pKa = 4.31SNVRR267 pKa = 11.84NMYY270 pKa = 9.89EE271 pKa = 3.61FSFAANHH278 pKa = 4.94YY279 pKa = 9.87CRR281 pKa = 11.84YY282 pKa = 9.22EE283 pKa = 4.17VNGPLANAAEE293 pKa = 4.54PVPTAPISVIDD304 pKa = 3.87GARR307 pKa = 11.84KK308 pKa = 8.68FVEE311 pKa = 4.24NVGSLGHH318 pKa = 5.41VVEE321 pKa = 5.15DD322 pKa = 3.71VVGTINAYY330 pKa = 10.24APAGLL335 pKa = 4.01

Molecular weight: 36.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1175

335

840

587.5

65.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.043 ± 0.374

1.447 ± 0.545

3.915 ± 0.016

6.638 ± 1.166

4.085 ± 0.186

7.915 ± 0.776

2.553 ± 0.063

3.574 ± 0.563

5.532 ± 0.925

7.404 ± 0.963

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.319 ± 0.125

4.0 ± 1.076

6.128 ± 0.208

2.638 ± 0.306

7.149 ± 0.857

6.383 ± 0.37

6.043 ± 0.249

6.894 ± 0.27

2.213 ± 0.342

2.128 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski