Brevundimonas sp. MYb31

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; unclassified Brevundimonas

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3210 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S8WAN0|A0A2S8WAN0_9CAUL Polysaccharide biosynthesis protein OS=Brevundimonas sp. MYb31 OX=1848616 GN=CQ026_02385 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84LALACASLLALTVGAGAAQAQSAPEE27 pKa = 3.73VAFNIGVVSDD37 pKa = 3.66YY38 pKa = 11.09VFRR41 pKa = 11.84GASQTDD47 pKa = 3.8EE48 pKa = 5.11DD49 pKa = 4.68PALQGGIDD57 pKa = 3.4ATFGGFYY64 pKa = 10.32AGTWASMVDD73 pKa = 4.5FGDD76 pKa = 3.97DD77 pKa = 2.89TDD79 pKa = 5.71AEE81 pKa = 4.16VDD83 pKa = 3.69LYY85 pKa = 11.39GGYY88 pKa = 8.19RR89 pKa = 11.84TEE91 pKa = 4.05AAGYY95 pKa = 9.41ALDD98 pKa = 4.2FGVVGYY104 pKa = 8.54FYY106 pKa = 11.39VNEE109 pKa = 4.23PSAADD114 pKa = 3.39YY115 pKa = 9.78TYY117 pKa = 11.59VEE119 pKa = 5.28FKK121 pKa = 10.69AAASRR126 pKa = 11.84AIGPATVGAAVYY138 pKa = 8.32YY139 pKa = 10.71SPDD142 pKa = 3.13FFGVDD147 pKa = 3.67DD148 pKa = 3.89EE149 pKa = 4.53ATYY152 pKa = 11.32LEE154 pKa = 4.76ANAAYY159 pKa = 10.38ALADD163 pKa = 3.08KK164 pKa = 8.41WTVSGAVGHH173 pKa = 5.67QWLNVTDD180 pKa = 6.25DD181 pKa = 3.68YY182 pKa = 10.0TTWNVGLAYY191 pKa = 10.53ALTDD195 pKa = 3.5NLAVDD200 pKa = 4.03VRR202 pKa = 11.84YY203 pKa = 10.12HH204 pKa = 6.19DD205 pKa = 3.93TDD207 pKa = 3.03VDD209 pKa = 4.47GPLTDD214 pKa = 3.9DD215 pKa = 3.8RR216 pKa = 11.84FVVGLKK222 pKa = 10.64ASFF225 pKa = 3.45

Molecular weight: 23.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S8W634|A0A2S8W634_9CAUL Pilus assembly protein TadE OS=Brevundimonas sp. MYb31 OX=1848616 GN=CQ026_09650 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.2NGQKK29 pKa = 9.53IVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3210

0

3210

1021726

39

4438

318.3

34.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.825 ± 0.068

0.685 ± 0.013

5.986 ± 0.036

5.605 ± 0.042

3.495 ± 0.026

8.935 ± 0.042

1.801 ± 0.025

4.444 ± 0.033

2.909 ± 0.035

9.961 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.019

2.447 ± 0.032

5.362 ± 0.033

3.209 ± 0.026

7.292 ± 0.044

5.145 ± 0.037

5.353 ± 0.032

7.65 ± 0.04

1.474 ± 0.018

2.108 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski