Hubei sobemo-like virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEN9|A0A1L3KEN9_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 7 OX=1923240 PE=4 SV=1
MM1 pKa = 7.1FVSSNVKK8 pKa = 10.26AMEE11 pKa = 4.23QIEE14 pKa = 4.51MVLTMMMVSLVVTLSVKK31 pKa = 9.82MLTEE35 pKa = 3.84LVRR38 pKa = 11.84WYY40 pKa = 10.67LRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84AQYY47 pKa = 10.93LYY49 pKa = 11.03AKK51 pKa = 9.54KK52 pKa = 10.34QEE54 pKa = 4.27DD55 pKa = 3.84LGRR58 pKa = 11.84LPEE61 pKa = 4.11SMRR64 pKa = 11.84EE65 pKa = 3.95GSDD68 pKa = 3.37LVTVPLANFPFVVGLYY84 pKa = 8.78SVRR87 pKa = 11.84RR88 pKa = 11.84QGAAEE93 pKa = 3.95KK94 pKa = 10.43VYY96 pKa = 10.88LGTGTLLEE104 pKa = 4.58GWLVTAGHH112 pKa = 5.38VVEE115 pKa = 4.89TAAGRR120 pKa = 11.84DD121 pKa = 3.92MFALTYY127 pKa = 10.67DD128 pKa = 3.9GVYY131 pKa = 10.45HH132 pKa = 6.93EE133 pKa = 5.86LGQFHH138 pKa = 7.32TIYY141 pKa = 10.27TDD143 pKa = 3.46LVMTKK148 pKa = 10.25APQGYY153 pKa = 9.28KK154 pKa = 9.76SGRR157 pKa = 11.84VEE159 pKa = 4.02ALQLASYY166 pKa = 8.99AQLVAARR173 pKa = 11.84GTANSSMGILKK184 pKa = 10.05HH185 pKa = 5.67DD186 pKa = 3.68TQVAFGFCSYY196 pKa = 10.52SGSSVQGFSGAPYY209 pKa = 8.9TNGSKK214 pKa = 10.43VLAIHH219 pKa = 6.92LGGGTSGNYY228 pKa = 9.69GYY230 pKa = 10.46SASFVMAQIHH240 pKa = 5.46VYY242 pKa = 9.77KK243 pKa = 10.39RR244 pKa = 11.84PEE246 pKa = 3.97SSEE249 pKa = 4.04LEE251 pKa = 4.01AMEE254 pKa = 5.0RR255 pKa = 11.84AMKK258 pKa = 10.03SAKK261 pKa = 10.26KK262 pKa = 10.24EE263 pKa = 4.14DD264 pKa = 3.82IEE266 pKa = 4.23WEE268 pKa = 4.0RR269 pKa = 11.84GLDD272 pKa = 3.43EE273 pKa = 3.89TRR275 pKa = 11.84VRR277 pKa = 11.84VGGRR281 pKa = 11.84YY282 pKa = 7.37FTLDD286 pKa = 2.97NDD288 pKa = 4.61EE289 pKa = 4.03FDD291 pKa = 3.82EE292 pKa = 4.35WSEE295 pKa = 3.92LNEE298 pKa = 4.1FGEE301 pKa = 4.18WFYY304 pKa = 11.68DD305 pKa = 3.64EE306 pKa = 5.06EE307 pKa = 5.28PEE309 pKa = 4.46SKK311 pKa = 10.24RR312 pKa = 11.84KK313 pKa = 9.44RR314 pKa = 11.84FRR316 pKa = 11.84VKK318 pKa = 10.27RR319 pKa = 11.84KK320 pKa = 9.4AVDD323 pKa = 3.56YY324 pKa = 10.35EE325 pKa = 4.16EE326 pKa = 5.38PDD328 pKa = 3.61YY329 pKa = 11.3EE330 pKa = 4.33PEE332 pKa = 4.2GVEE335 pKa = 5.57DD336 pKa = 4.83PFLEE340 pKa = 4.44SKK342 pKa = 10.21PPKK345 pKa = 9.89DD346 pKa = 3.67SSSGALEE353 pKa = 3.86KK354 pKa = 10.51RR355 pKa = 11.84IEE357 pKa = 3.98LLAEE361 pKa = 4.53NIASIQSLLTSQSQDD376 pKa = 2.83MGGLKK381 pKa = 9.96EE382 pKa = 4.51RR383 pKa = 11.84LNKK386 pKa = 10.14LDD388 pKa = 3.72TACVSMRR395 pKa = 11.84NIEE398 pKa = 4.39SKK400 pKa = 10.73LSEE403 pKa = 4.27TFASQLTMSVNTLEE417 pKa = 4.37QRR419 pKa = 11.84LTAQIKK425 pKa = 9.52SVSDD429 pKa = 3.52ARR431 pKa = 11.84PHH433 pKa = 6.22PEE435 pKa = 3.64KK436 pKa = 10.02TASTNGSDD444 pKa = 3.36TSSPPSIPEE453 pKa = 3.82VPQVSVSSGSTQPSVMRR470 pKa = 11.84WATMDD475 pKa = 3.41SDD477 pKa = 4.09FATMLEE483 pKa = 3.87WRR485 pKa = 11.84NSVDD489 pKa = 3.19RR490 pKa = 11.84SSPSYY495 pKa = 8.07PHH497 pKa = 6.18WRR499 pKa = 11.84EE500 pKa = 3.29EE501 pKa = 3.98YY502 pKa = 7.48MTQKK506 pKa = 10.68GFSTEE511 pKa = 3.77QKK513 pKa = 8.86KK514 pKa = 10.81ALVVRR519 pKa = 11.84LKK521 pKa = 10.45NYY523 pKa = 9.69KK524 pKa = 9.78VSSQRR529 pKa = 11.84KK530 pKa = 4.8VARR533 pKa = 11.84KK534 pKa = 9.35AKK536 pKa = 10.39SAIQQ540 pKa = 3.27

Molecular weight: 60.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEN9|A0A1L3KEN9_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 7 OX=1923240 PE=4 SV=1
MM1 pKa = 7.77VDD3 pKa = 3.23RR4 pKa = 11.84CLMHH8 pKa = 6.86PFIQSEE14 pKa = 4.03IRR16 pKa = 11.84NPMDD20 pKa = 3.43VVSKK24 pKa = 10.94AGWAPLPAGYY34 pKa = 10.28QRR36 pKa = 11.84MIAEE40 pKa = 4.38FPEE43 pKa = 4.18KK44 pKa = 9.67QAYY47 pKa = 10.11AIDD50 pKa = 3.75KK51 pKa = 10.41KK52 pKa = 10.9LWDD55 pKa = 3.4WTMPGWVVLEE65 pKa = 3.92YY66 pKa = 11.14VLAKK70 pKa = 10.35LRR72 pKa = 11.84QCRR75 pKa = 11.84EE76 pKa = 3.71HH77 pKa = 6.85SLLWEE82 pKa = 3.71WMVWRR87 pKa = 11.84RR88 pKa = 11.84IYY90 pKa = 11.16YY91 pKa = 9.86SFGPGAVLAEE101 pKa = 4.0PTGAIWRR108 pKa = 11.84QTFWGLMKK116 pKa = 10.57SGSLLTLSMNGASQYY131 pKa = 10.93LEE133 pKa = 4.8HH134 pKa = 6.87ALAWLRR140 pKa = 11.84MLDD143 pKa = 3.98GGPISWPPRR152 pKa = 11.84MWAMGDD158 pKa = 3.35DD159 pKa = 3.53TLARR163 pKa = 11.84MNSEE167 pKa = 3.7WVDD170 pKa = 3.68GYY172 pKa = 11.34LRR174 pKa = 11.84EE175 pKa = 4.04LAKK178 pKa = 10.08TGCIVKK184 pKa = 10.05LAEE187 pKa = 3.91NSRR190 pKa = 11.84EE191 pKa = 3.73FSGFRR196 pKa = 11.84VEE198 pKa = 5.06GDD200 pKa = 3.5SVANAIVTPIYY211 pKa = 9.75PEE213 pKa = 3.61KK214 pKa = 10.78HH215 pKa = 5.54KK216 pKa = 10.88FLVKK220 pKa = 10.31HH221 pKa = 5.46VRR223 pKa = 11.84AEE225 pKa = 4.2DD226 pKa = 3.46EE227 pKa = 4.24HH228 pKa = 8.63SVFLSFSLLYY238 pKa = 10.92ALARR242 pKa = 11.84PGWLNNVIGRR252 pKa = 11.84TDD254 pKa = 3.29VTVGPMQRR262 pKa = 11.84LWAKK266 pKa = 10.59GMAKK270 pKa = 10.45LEE272 pKa = 4.31LFDD275 pKa = 5.41TLPEE279 pKa = 3.89WTRR282 pKa = 11.84FWDD285 pKa = 3.36

Molecular weight: 32.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

825

285

540

412.5

46.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.758 ± 0.351

0.606 ± 0.236

4.364 ± 0.105

7.758 ± 0.763

3.515 ± 0.182

6.788 ± 0.122

1.697 ± 0.216

2.909 ± 0.503

5.576 ± 0.537

9.212 ± 0.881

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.242 ± 0.355

2.545 ± 0.047

4.485 ± 0.598

3.636 ± 0.625

6.303 ± 0.192

9.091 ± 1.84

5.212 ± 0.716

7.515 ± 0.449

3.03 ± 1.553

3.758 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski