Streptococcus phage IPP10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5S872|A0A1S5S872_9CAUD Lytic amidase OS=Streptococcus phage IPP10 OX=1916151 GN=IPP10_00063 PE=4 SV=1
MM1 pKa = 7.51SRR3 pKa = 11.84LQPRR7 pKa = 11.84PEE9 pKa = 3.91KK10 pKa = 10.78NYY12 pKa = 10.37PNYY15 pKa = 10.85NWDD18 pKa = 3.65DD19 pKa = 4.05LDD21 pKa = 5.81RR22 pKa = 11.84FIQDD26 pKa = 3.37VLSNPAFKK34 pKa = 10.7VCCVDD39 pKa = 3.32PKK41 pKa = 11.04LYY43 pKa = 10.48AIPKK47 pKa = 9.93DD48 pKa = 4.05EE49 pKa = 5.58IISEE53 pKa = 4.61CISAGYY59 pKa = 9.87AVEE62 pKa = 3.91EE63 pKa = 4.49RR64 pKa = 11.84EE65 pKa = 4.85DD66 pKa = 4.31GILNISS72 pKa = 3.4

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5S861|A0A1S5S861_9CAUD Capsid and scaffold protein OS=Streptococcus phage IPP10 OX=1916151 GN=IPP10_00045 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 9.42PYY8 pKa = 9.73SGKK11 pKa = 9.53IKK13 pKa = 10.6KK14 pKa = 9.77PIEE17 pKa = 3.75QAIDD21 pKa = 3.35LLEE24 pKa = 4.78RR25 pKa = 11.84ISRR28 pKa = 11.84LEE30 pKa = 3.87SQVISLANHH39 pKa = 5.71EE40 pKa = 4.41MFKK43 pKa = 10.41IPSSRR48 pKa = 11.84SSTAA52 pKa = 2.78

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12942

34

2402

205.4

23.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.846 ± 0.586

0.525 ± 0.084

6.158 ± 0.314

8.028 ± 0.509

4.157 ± 0.251

6.112 ± 0.521

1.422 ± 0.189

6.39 ± 0.247

8.87 ± 0.427

8.244 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.25

5.44 ± 0.307

2.789 ± 0.259

3.979 ± 0.206

4.605 ± 0.301

6.29 ± 0.417

6.236 ± 0.332

6.212 ± 0.261

1.344 ± 0.153

3.871 ± 0.38
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski