Mitsuokella sp. oral taxon 131 str. W9106

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Selenomonadaceae; Mitsuokella; unclassified Mitsuokella; Mitsuokella sp. oral taxon 131

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2TDG3|U2TDG3_9FIRM 30S ribosomal protein S4 OS=Mitsuokella sp. oral taxon 131 str. W9106 OX=1321781 GN=rpsD PE=3 SV=1
MM1 pKa = 7.93AYY3 pKa = 10.47GIMILAEE10 pKa = 4.05RR11 pKa = 11.84RR12 pKa = 11.84DD13 pKa = 3.72GDD15 pKa = 3.57MEE17 pKa = 4.71RR18 pKa = 11.84YY19 pKa = 7.44CTNCGKK25 pKa = 9.56PLKK28 pKa = 10.7AVDD31 pKa = 4.5RR32 pKa = 11.84FCANCGKK39 pKa = 9.4PCPISSRR46 pKa = 11.84EE47 pKa = 3.67RR48 pKa = 11.84AAPSCSAVRR57 pKa = 11.84RR58 pKa = 11.84EE59 pKa = 4.17EE60 pKa = 4.43PSGLWKK66 pKa = 10.36FLAGSALGVMVANLFGHH83 pKa = 7.33ASHH86 pKa = 7.8DD87 pKa = 3.77AQANAMAQASAADD100 pKa = 3.63HH101 pKa = 6.34HH102 pKa = 6.77AQGGDD107 pKa = 3.3ADD109 pKa = 3.86YY110 pKa = 11.25DD111 pKa = 3.89AYY113 pKa = 11.47LEE115 pKa = 4.66GYY117 pKa = 10.03RR118 pKa = 11.84EE119 pKa = 4.11GHH121 pKa = 6.12QDD123 pKa = 4.14ALDD126 pKa = 4.03DD127 pKa = 4.44DD128 pKa = 4.37FRR130 pKa = 11.84EE131 pKa = 4.19PVDD134 pKa = 4.75YY135 pKa = 11.15GDD137 pKa = 5.08EE138 pKa = 4.58DD139 pKa = 4.46DD140 pKa = 5.0ADD142 pKa = 3.77AYY144 pKa = 10.69AYY146 pKa = 10.54DD147 pKa = 4.12GGRR150 pKa = 11.84DD151 pKa = 3.77DD152 pKa = 6.46DD153 pKa = 4.3MDD155 pKa = 4.16MDD157 pKa = 5.13SDD159 pKa = 5.58CDD161 pKa = 4.05DD162 pKa = 4.96DD163 pKa = 7.06CDD165 pKa = 4.66DD166 pKa = 5.23GYY168 pKa = 11.89GDD170 pKa = 5.2GYY172 pKa = 11.3DD173 pKa = 5.72DD174 pKa = 6.88FDD176 pKa = 6.25DD177 pKa = 5.29CDD179 pKa = 5.46DD180 pKa = 4.34YY181 pKa = 12.14DD182 pKa = 4.98EE183 pKa = 5.11EE184 pKa = 4.84DD185 pKa = 3.22

Molecular weight: 20.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2UKR7|U2UKR7_9FIRM Peptidase M16 inactive domain protein OS=Mitsuokella sp. oral taxon 131 str. W9106 OX=1321781 GN=HMPREF1985_00050 PE=4 SV=1
MM1 pKa = 7.12KK2 pKa = 9.29QTYY5 pKa = 9.09QPNNHH10 pKa = 5.49WRR12 pKa = 11.84KK13 pKa = 7.63KK14 pKa = 6.32THH16 pKa = 5.67GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.85TKK25 pKa = 10.22GGRR28 pKa = 11.84LVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2309

0

2309

714581

38

3584

309.5

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.799 ± 0.083

1.286 ± 0.024

5.429 ± 0.043

6.622 ± 0.057

3.966 ± 0.048

7.833 ± 0.059

2.314 ± 0.019

6.275 ± 0.056

5.295 ± 0.054

9.387 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.094 ± 0.024

3.015 ± 0.042

3.887 ± 0.035

3.088 ± 0.031

6.048 ± 0.06

5.197 ± 0.035

5.231 ± 0.052

7.15 ± 0.047

0.935 ± 0.018

3.148 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski