Mycobacterium phage Tortellini

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tortellinivirus; Mycobacterium virus Tortellini

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8EX09|A0A1D8EX09_9CAUD Head-to-tail adaptor OS=Mycobacterium phage Tortellini OX=1897497 GN=SEA_TORTELLINI_8 PE=4 SV=1
MM1 pKa = 7.89AYY3 pKa = 10.34AEE5 pKa = 4.98LLDD8 pKa = 3.63TDD10 pKa = 3.86MTTWCPITVHH20 pKa = 6.02YY21 pKa = 10.33KK22 pKa = 9.12VTDD25 pKa = 3.89GDD27 pKa = 3.94QVSYY31 pKa = 10.88LAVTRR36 pKa = 11.84VSFVTATGRR45 pKa = 11.84VEE47 pKa = 5.67AFACDD52 pKa = 3.56EE53 pKa = 4.44TGVATSLAPVWAIDD67 pKa = 3.66GDD69 pKa = 4.14VGHH72 pKa = 6.94NDD74 pKa = 3.21ALVAAGYY81 pKa = 7.62TVII84 pKa = 5.23

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8EX60|A0A1D8EX60_9CAUD DNA polymerase III sliding clamp OS=Mycobacterium phage Tortellini OX=1897497 GN=SEA_TORTELLINI_57 PE=4 SV=1
MM1 pKa = 7.31SRR3 pKa = 11.84RR4 pKa = 11.84IIHH7 pKa = 5.43RR8 pKa = 11.84TVHH11 pKa = 5.58KK12 pKa = 9.4RR13 pKa = 11.84TARR16 pKa = 11.84RR17 pKa = 11.84TLALTGLGALAAAGIITATPAEE39 pKa = 4.64ALPGQCGGGPLVGFPTDD56 pKa = 3.95NDD58 pKa = 4.09PATPCC63 pKa = 4.22

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15761

44

1229

207.4

22.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.207 ± 0.54

1.041 ± 0.148

6.649 ± 0.206

6.269 ± 0.328

2.919 ± 0.206

9.048 ± 0.616

1.872 ± 0.184

4.549 ± 0.204

3.363 ± 0.179

7.76 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.891 ± 0.099

2.823 ± 0.172

5.989 ± 0.201

3.877 ± 0.286

6.935 ± 0.414

5.196 ± 0.192

6.288 ± 0.249

7.011 ± 0.264

1.922 ± 0.162

2.392 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski