Sierra Nevada virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Nyamiviridae; Nyavirus; Sierra Nevada nyavirus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067YEW0|A0A067YEW0_9MONO P protein OS=Sierra Nevada virus OX=1424280 GN=SenV_gp4 PE=4 SV=1
MM1 pKa = 8.25DD2 pKa = 3.73YY3 pKa = 10.47HH4 pKa = 6.07VHH6 pKa = 6.32RR7 pKa = 11.84HH8 pKa = 5.93HH9 pKa = 7.12DD10 pKa = 3.29QCKK13 pKa = 8.8QGEE16 pKa = 4.39NQDD19 pKa = 3.47HH20 pKa = 6.24SEE22 pKa = 3.97EE23 pKa = 4.48SEE25 pKa = 3.99IEE27 pKa = 3.98LLISEE32 pKa = 4.55SEE34 pKa = 4.19EE35 pKa = 4.01EE36 pKa = 4.21EE37 pKa = 4.14EE38 pKa = 4.32GAEE41 pKa = 4.04TGEE44 pKa = 4.42EE45 pKa = 4.24GAEE48 pKa = 3.92AEE50 pKa = 4.0PSKK53 pKa = 11.02RR54 pKa = 11.84EE55 pKa = 3.78EE56 pKa = 4.11EE57 pKa = 4.13EE58 pKa = 3.72AGEE61 pKa = 4.04RR62 pKa = 11.84DD63 pKa = 3.43EE64 pKa = 6.27SEE66 pKa = 4.54TEE68 pKa = 4.11TSDD71 pKa = 3.63SSSNEE76 pKa = 3.79DD77 pKa = 3.51PQVEE81 pKa = 4.33EE82 pKa = 4.99PIRR85 pKa = 11.84ATRR88 pKa = 11.84APLPLYY94 pKa = 10.7GNSPEE99 pKa = 4.01QDD101 pKa = 2.66RR102 pKa = 11.84KK103 pKa = 10.8AEE105 pKa = 4.06RR106 pKa = 11.84LISAYY111 pKa = 9.53KK112 pKa = 10.1ALRR115 pKa = 11.84HH116 pKa = 6.04KK117 pKa = 10.56EE118 pKa = 4.11SEE120 pKa = 4.34SDD122 pKa = 3.76SEE124 pKa = 4.37TDD126 pKa = 3.18SSEE129 pKa = 4.08DD130 pKa = 2.97ATATKK135 pKa = 10.28RR136 pKa = 11.84PRR138 pKa = 11.84PCYY141 pKa = 9.41GDD143 pKa = 3.23RR144 pKa = 11.84RR145 pKa = 11.84FEE147 pKa = 4.11KK148 pKa = 10.78LVMEE152 pKa = 5.54CYY154 pKa = 9.93NAYY157 pKa = 10.4DD158 pKa = 4.26PNTDD162 pKa = 3.09AAANLMMRR170 pKa = 11.84RR171 pKa = 11.84KK172 pKa = 9.2FLHH175 pKa = 4.77WQEE178 pKa = 4.23NILRR182 pKa = 11.84LMSSRR187 pKa = 11.84DD188 pKa = 3.62RR189 pKa = 11.84ALHH192 pKa = 6.6DD193 pKa = 3.77YY194 pKa = 10.22GRR196 pKa = 11.84QASNITATRR205 pKa = 11.84RR206 pKa = 11.84LEE208 pKa = 3.96VGADD212 pKa = 2.87RR213 pKa = 11.84FYY215 pKa = 11.32RR216 pKa = 11.84WRR218 pKa = 11.84NLIALEE224 pKa = 4.15EE225 pKa = 3.98EE226 pKa = 4.42LAIKK230 pKa = 10.36VPLVLSLHH238 pKa = 5.93LEE240 pKa = 4.01EE241 pKa = 5.86RR242 pKa = 11.84SAAQHH247 pKa = 5.61LAEE250 pKa = 5.53DD251 pKa = 4.24EE252 pKa = 4.41EE253 pKa = 4.76LGKK256 pKa = 10.64ALDD259 pKa = 4.11KK260 pKa = 11.36LSLDD264 pKa = 3.17EE265 pKa = 4.23SEE267 pKa = 4.63RR268 pKa = 11.84RR269 pKa = 11.84RR270 pKa = 11.84ASTT273 pKa = 3.31

Molecular weight: 31.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067YFD9|A0A067YFD9_9MONO M protein OS=Sierra Nevada virus OX=1424280 GN=SenV_gp3 PE=4 SV=1
MM1 pKa = 7.26NKK3 pKa = 9.77EE4 pKa = 3.88KK5 pKa = 10.84GKK7 pKa = 9.31ATIWYY12 pKa = 9.67QMIAKK17 pKa = 8.02TPQKK21 pKa = 9.93RR22 pKa = 11.84YY23 pKa = 9.91VVTIQLLSVPSKK35 pKa = 11.46NEE37 pKa = 3.69FEE39 pKa = 5.03DD40 pKa = 4.3RR41 pKa = 11.84PHH43 pKa = 6.79PALFEE48 pKa = 4.21GSKK51 pKa = 10.81ACIFIHH57 pKa = 6.91AGEE60 pKa = 3.75AHH62 pKa = 6.48ARR64 pKa = 11.84IEE66 pKa = 4.4LDD68 pKa = 3.21PEE70 pKa = 4.34KK71 pKa = 10.66YY72 pKa = 10.4AEE74 pKa = 5.06LGTGMEE80 pKa = 4.31PCQVYY85 pKa = 10.25CLKK88 pKa = 10.75QYY90 pKa = 9.16GWKK93 pKa = 8.76PRR95 pKa = 11.84HH96 pKa = 5.72FAGHH100 pKa = 5.94PLKK103 pKa = 10.52VTFDD107 pKa = 3.73KK108 pKa = 11.04IIFKK112 pKa = 10.65EE113 pKa = 4.09PAALMLNTKK122 pKa = 10.15SSAFFFKK129 pKa = 10.48RR130 pKa = 11.84KK131 pKa = 7.94EE132 pKa = 3.67KK133 pKa = 10.34RR134 pKa = 11.84RR135 pKa = 11.84EE136 pKa = 3.71KK137 pKa = 11.24GEE139 pKa = 3.9MRR141 pKa = 11.84DD142 pKa = 3.51EE143 pKa = 4.14RR144 pKa = 5.09

Molecular weight: 16.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3682

144

1917

613.7

69.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.605 ± 1.048

2.146 ± 0.512

4.807 ± 0.342

8.365 ± 1.745

3.069 ± 0.635

5.866 ± 0.326

2.797 ± 0.397

4.237 ± 0.331

5.839 ± 1.135

11.407 ± 1.635

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.064 ± 0.201

2.988 ± 0.445

5.432 ± 0.394

3.992 ± 0.268

6.898 ± 0.857

7.089 ± 0.717

6.247 ± 0.479

4.454 ± 0.613

1.711 ± 0.387

2.988 ± 0.41
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski