Salinicola sp. MIT1003

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Salinicola; unclassified Salinicola

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3468 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S2DHH7|A0A1S2DHH7_9GAMM Phosphonate metabolism protein PhnP OS=Salinicola sp. MIT1003 OX=1882734 GN=BC443_05575 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.47KK3 pKa = 8.85STTGLLLGSALVVGLSGCASSGMEE27 pKa = 4.17STGSSSDD34 pKa = 3.52QAWYY38 pKa = 10.29QSPFVCGIAGGLIGGGVGYY57 pKa = 8.28ATSGDD62 pKa = 3.72SDD64 pKa = 4.36EE65 pKa = 4.74DD66 pKa = 3.27TGAAVGGVAGATAGALLCADD86 pKa = 3.97YY87 pKa = 11.17SSDD90 pKa = 4.56VMDD93 pKa = 4.36SDD95 pKa = 4.8GDD97 pKa = 4.09GVPDD101 pKa = 6.13DD102 pKa = 5.8RR103 pKa = 11.84DD104 pKa = 3.43QCPNTPAGVAVDD116 pKa = 4.79AVGCPLDD123 pKa = 3.62TDD125 pKa = 4.11GDD127 pKa = 4.39GVPDD131 pKa = 5.28YY132 pKa = 10.64MDD134 pKa = 3.44QCPGTPAGVEE144 pKa = 4.28VNAQGCPLDD153 pKa = 3.75SDD155 pKa = 4.27GDD157 pKa = 4.25GVPDD161 pKa = 4.49YY162 pKa = 11.09QDD164 pKa = 3.21QCPDD168 pKa = 3.29TPAGAEE174 pKa = 4.21VNSLGCVEE182 pKa = 5.07DD183 pKa = 3.92LVLRR187 pKa = 11.84DD188 pKa = 3.48VNFEE192 pKa = 3.85FDD194 pKa = 4.0SAQLTANAEE203 pKa = 4.32AILDD207 pKa = 4.06GVSEE211 pKa = 4.38KK212 pKa = 11.14LMTNDD217 pKa = 3.02QLRR220 pKa = 11.84IRR222 pKa = 11.84LEE224 pKa = 3.85GHH226 pKa = 5.96TDD228 pKa = 3.22SVGPAAYY235 pKa = 10.02NKK237 pKa = 9.91EE238 pKa = 3.89LSQRR242 pKa = 11.84RR243 pKa = 11.84ADD245 pKa = 3.64SVKK248 pKa = 10.6SYY250 pKa = 10.88LVNKK254 pKa = 9.28GFSADD259 pKa = 3.56NITTIGYY266 pKa = 10.17GEE268 pKa = 4.27EE269 pKa = 4.06QPVASNDD276 pKa = 3.64TAEE279 pKa = 4.3GRR281 pKa = 11.84AQNRR285 pKa = 11.84RR286 pKa = 11.84VEE288 pKa = 4.13LGEE291 pKa = 3.99WEE293 pKa = 4.08

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2DCE5|A0A1S2DCE5_9GAMM GntR family transcriptional regulator OS=Salinicola sp. MIT1003 OX=1882734 GN=BC443_03945 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.42NGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3468

0

3468

1139571

43

2602

328.6

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.971 ± 0.047

0.915 ± 0.012

6.058 ± 0.04

6.179 ± 0.042

3.509 ± 0.024

8.146 ± 0.039

2.399 ± 0.024

4.986 ± 0.034

2.771 ± 0.035

11.034 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.019

2.658 ± 0.025

4.876 ± 0.025

3.975 ± 0.033

7.25 ± 0.044

5.733 ± 0.031

5.057 ± 0.03

7.065 ± 0.033

1.513 ± 0.019

2.436 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski