Wellfleet Bay virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Quaranjavirus; unclassified Quaranjavirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A1E6X3|A0A0A1E6X3_9ORTO RNA-directed RNA polymerase catalytic subunit OS=Wellfleet Bay virus OX=1566309 GN=PB1 PE=4 SV=1
MM1 pKa = 7.35AFEE4 pKa = 4.77VYY6 pKa = 10.13RR7 pKa = 11.84LAISNPILYY16 pKa = 9.92DD17 pKa = 3.35PRR19 pKa = 11.84FVSVAGEE26 pKa = 4.12NSEE29 pKa = 3.99HH30 pKa = 6.73WPRR33 pKa = 11.84DD34 pKa = 3.06NWKK37 pKa = 10.16KK38 pKa = 10.38RR39 pKa = 11.84EE40 pKa = 3.66EE41 pKa = 4.3SLRR44 pKa = 11.84HH45 pKa = 5.62DD46 pKa = 3.85QVCALLMNTASRR58 pKa = 11.84FPSHH62 pKa = 6.97LEE64 pKa = 3.49AAKK67 pKa = 10.19EE68 pKa = 4.15SSSSKK73 pKa = 8.95SQIYY77 pKa = 10.23EE78 pKa = 3.86EE79 pKa = 4.98GEE81 pKa = 3.88KK82 pKa = 10.54QFNQEE87 pKa = 4.04EE88 pKa = 4.59IEE90 pKa = 4.17GLMEE94 pKa = 5.28AGQMHH99 pKa = 6.72LTEE102 pKa = 4.69SFSTSVRR109 pKa = 11.84QDD111 pKa = 3.43DD112 pKa = 4.07EE113 pKa = 4.64EE114 pKa = 4.32EE115 pKa = 4.53SEE117 pKa = 4.67GEE119 pKa = 4.15GGEE122 pKa = 4.53SGDD125 pKa = 5.41DD126 pKa = 3.67EE127 pKa = 6.4DD128 pKa = 6.53DD129 pKa = 3.45EE130 pKa = 5.15FAIGDD135 pKa = 3.46YY136 pKa = 9.71WGPPEE141 pKa = 3.91QTEE144 pKa = 4.24EE145 pKa = 4.11EE146 pKa = 4.34MEE148 pKa = 3.98EE149 pKa = 4.17MINVGEE155 pKa = 4.25PDD157 pKa = 4.43LVDD160 pKa = 3.22SDD162 pKa = 3.4KK163 pKa = 11.12RR164 pKa = 11.84YY165 pKa = 8.65TILEE169 pKa = 4.27GVTGAKK175 pKa = 8.03YY176 pKa = 7.98TQIAFSTLWGIDD188 pKa = 3.33TQKK191 pKa = 10.37QWDD194 pKa = 4.14IIDD197 pKa = 3.51KK198 pKa = 9.13QEE200 pKa = 3.77RR201 pKa = 11.84KK202 pKa = 9.79FIEE205 pKa = 4.05VKK207 pKa = 8.55ITSRR211 pKa = 11.84NPYY214 pKa = 8.65QVWEE218 pKa = 4.28EE219 pKa = 4.12VKK221 pKa = 10.39SASDD225 pKa = 2.86ISEE228 pKa = 4.05EE229 pKa = 4.12HH230 pKa = 6.23FGACIVHH237 pKa = 7.09DD238 pKa = 4.17EE239 pKa = 4.62GGGSFKK245 pKa = 10.88QYY247 pKa = 10.43VFNIDD252 pKa = 4.27EE253 pKa = 4.3MPGWKK258 pKa = 9.32QVEE261 pKa = 4.23DD262 pKa = 3.82FLIRR266 pKa = 11.84RR267 pKa = 11.84YY268 pKa = 10.42EE269 pKa = 3.84FFLEE273 pKa = 4.16NGAVPVAGGDD283 pKa = 3.54EE284 pKa = 3.98DD285 pKa = 5.8RR286 pKa = 11.84YY287 pKa = 10.21PVWDD291 pKa = 4.72PIISEE296 pKa = 5.02KK297 pKa = 9.68IHH299 pKa = 7.04DD300 pKa = 4.85WIHH303 pKa = 6.53PLWQPDD309 pKa = 3.93KK310 pKa = 11.18INARR314 pKa = 11.84VDD316 pKa = 3.06NSPRR320 pKa = 11.84YY321 pKa = 8.87EE322 pKa = 4.02PFNIGLFLNLLDD334 pKa = 5.09DD335 pKa = 4.24PRR337 pKa = 11.84ARR339 pKa = 11.84NCSKK343 pKa = 9.93SAKK346 pKa = 9.16WNGKK350 pKa = 9.19LMPFSWNSAVATTCDD365 pKa = 3.41KK366 pKa = 11.27DD367 pKa = 4.0LDD369 pKa = 3.98MVEE372 pKa = 4.09EE373 pKa = 4.8IIRR376 pKa = 11.84HH377 pKa = 5.39FDD379 pKa = 3.47CEE381 pKa = 4.18SWSVVNWKK389 pKa = 9.03EE390 pKa = 3.73QSAEE394 pKa = 4.05SLVNGLKK401 pKa = 10.76LLLEE405 pKa = 4.22RR406 pKa = 11.84FKK408 pKa = 11.42EE409 pKa = 4.31GFIEE413 pKa = 4.26RR414 pKa = 11.84NSYY417 pKa = 10.56IPVKK421 pKa = 9.04KK422 pKa = 8.59TKK424 pKa = 10.44HH425 pKa = 4.16GWTVKK430 pKa = 10.36GEE432 pKa = 4.02RR433 pKa = 11.84HH434 pKa = 4.55TSEE437 pKa = 3.79IQEE440 pKa = 4.22MLSEE444 pKa = 5.35LGIGWKK450 pKa = 10.04FYY452 pKa = 9.88KK453 pKa = 9.37HH454 pKa = 5.42TGIVEE459 pKa = 3.84EE460 pKa = 5.39DD461 pKa = 3.08MRR463 pKa = 11.84QPEE466 pKa = 4.34SEE468 pKa = 4.36DD469 pKa = 3.41QEE471 pKa = 4.27MMRR474 pKa = 11.84WDD476 pKa = 3.58DD477 pKa = 3.25WLTEE481 pKa = 3.95LRR483 pKa = 11.84ISEE486 pKa = 4.31SQGLQGGRR494 pKa = 11.84VFKK497 pKa = 11.03DD498 pKa = 3.41DD499 pKa = 3.65VFKK502 pKa = 10.59EE503 pKa = 4.15APSFHH508 pKa = 7.44ILDD511 pKa = 3.97EE512 pKa = 4.22PAKK515 pKa = 10.12RR516 pKa = 11.84ICTEE520 pKa = 3.68LFDD523 pKa = 5.33LFRR526 pKa = 11.84THH528 pKa = 7.78KK529 pKa = 10.48IGATCAKK536 pKa = 9.95VMNFYY541 pKa = 11.19SRR543 pKa = 11.84MGGTYY548 pKa = 10.15LRR550 pKa = 11.84AANGGKK556 pKa = 9.97SKK558 pKa = 8.91MHH560 pKa = 6.63SSLAILPLYY569 pKa = 8.79YY570 pKa = 9.91KK571 pKa = 9.79EE572 pKa = 4.02YY573 pKa = 10.68RR574 pKa = 11.84GDD576 pKa = 3.75RR577 pKa = 11.84EE578 pKa = 4.15EE579 pKa = 4.18KK580 pKa = 8.1TSQRR584 pKa = 11.84VMVGFVVRR592 pKa = 11.84GPHH595 pKa = 5.89HH596 pKa = 6.92VRR598 pKa = 11.84DD599 pKa = 3.84ATDD602 pKa = 4.13TINLIIAEE610 pKa = 4.38KK611 pKa = 10.74VNLSLEE617 pKa = 4.05EE618 pKa = 3.98CKK620 pKa = 10.29RR621 pKa = 11.84RR622 pKa = 11.84LRR624 pKa = 11.84GGVLTSSGWFIRR636 pKa = 11.84KK637 pKa = 8.47NAIRR641 pKa = 11.84KK642 pKa = 9.1ADD644 pKa = 3.57PTYY647 pKa = 11.2LSFLHH652 pKa = 6.59NSLFVPVNFLGEE664 pKa = 5.04LITNHH669 pKa = 5.73PQRR672 pKa = 11.84SRR674 pKa = 11.84TKK676 pKa = 10.18EE677 pKa = 3.73DD678 pKa = 2.88PKK680 pKa = 11.01YY681 pKa = 10.2WDD683 pKa = 4.11VILAGCLTSCRR694 pKa = 11.84PFHH697 pKa = 7.06LEE699 pKa = 3.96RR700 pKa = 11.84IVEE703 pKa = 4.24TVLMGIIGKK712 pKa = 7.75SQEE715 pKa = 4.04EE716 pKa = 5.61GYY718 pKa = 10.96HH719 pKa = 6.41DD720 pKa = 4.5MLRR723 pKa = 11.84KK724 pKa = 10.15VYY726 pKa = 9.0MLLMARR732 pKa = 11.84GRR734 pKa = 11.84GEE736 pKa = 3.54IALVMDD742 pKa = 4.93LKK744 pKa = 11.69AMAEE748 pKa = 4.05EE749 pKa = 4.22MNEE752 pKa = 3.93CLINSAYY759 pKa = 10.01VFWVHH764 pKa = 7.2CEE766 pKa = 3.79LLDD769 pKa = 3.9FMKK772 pKa = 10.39FVKK775 pKa = 10.5DD776 pKa = 3.06KK777 pKa = 11.13TPFNEE782 pKa = 4.19HH783 pKa = 5.99LL784 pKa = 3.95

Molecular weight:
90.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A1E760|A0A0A1E760_9ORTO Matrix protein OS=Wellfleet Bay virus OX=1566309 GN=M PE=4 SV=1
MM1 pKa = 6.9QASPEE6 pKa = 4.28AIQARR11 pKa = 11.84KK12 pKa = 9.32EE13 pKa = 4.02RR14 pKa = 11.84LLKK17 pKa = 10.4LARR20 pKa = 11.84LVNQVAEE27 pKa = 4.45EE28 pKa = 4.28EE29 pKa = 4.32NGHH32 pKa = 7.08DD33 pKa = 4.9VLNLLKK39 pKa = 10.54KK40 pKa = 10.71EE41 pKa = 4.05PVCNKK46 pKa = 10.01RR47 pKa = 11.84VITKK51 pKa = 7.44YY52 pKa = 10.65ARR54 pKa = 11.84VVKK57 pKa = 10.51DD58 pKa = 3.76PDD60 pKa = 5.1PIATTQLLMGQKK72 pKa = 10.23FPILAKK78 pKa = 10.2KK79 pKa = 10.46SYY81 pKa = 10.03LRR83 pKa = 11.84YY84 pKa = 7.68YY85 pKa = 9.96TEE87 pKa = 4.23AEE89 pKa = 4.01RR90 pKa = 11.84EE91 pKa = 4.1EE92 pKa = 5.09LFSNDD97 pKa = 2.96DD98 pKa = 3.52CRR100 pKa = 11.84RR101 pKa = 11.84VGWVKK106 pKa = 10.63CSRR109 pKa = 11.84KK110 pKa = 10.28AIDD113 pKa = 3.14LWISKK118 pKa = 8.88GKK120 pKa = 9.42PLTEE124 pKa = 4.02EE125 pKa = 3.42QRR127 pKa = 11.84QIIKK131 pKa = 10.22VLYY134 pKa = 8.88EE135 pKa = 3.99SSIEE139 pKa = 4.03MAKK142 pKa = 10.59DD143 pKa = 4.3FYY145 pKa = 11.52SRR147 pKa = 11.84AWDD150 pKa = 3.68RR151 pKa = 11.84ATVTYY156 pKa = 10.63GRR158 pKa = 11.84VPLEE162 pKa = 4.13RR163 pKa = 11.84QSVATRR169 pKa = 11.84NVLVNVPRR177 pKa = 11.84EE178 pKa = 4.04YY179 pKa = 9.96RR180 pKa = 11.84QKK182 pKa = 10.75AVVEE186 pKa = 4.26IVQPGYY192 pKa = 10.96NLNQPEE198 pKa = 4.22VKK200 pKa = 9.82IYY202 pKa = 9.85VQQILEE208 pKa = 4.54KK209 pKa = 9.13IGQRR213 pKa = 11.84IVTGMSIVDD222 pKa = 3.44QARR225 pKa = 11.84ILMNSLDD232 pKa = 3.94PKK234 pKa = 10.71RR235 pKa = 11.84RR236 pKa = 11.84ILPVALSLHH245 pKa = 6.44GEE247 pKa = 4.52LAQHH251 pKa = 6.84SIAYY255 pKa = 9.33YY256 pKa = 9.58GNNWMITQLPGRR268 pKa = 11.84TYY270 pKa = 9.56STPGDD275 pKa = 3.81TYY277 pKa = 11.64DD278 pKa = 3.5MRR280 pKa = 11.84RR281 pKa = 11.84ICAFILSRR289 pKa = 11.84ISKK292 pKa = 10.62LEE294 pKa = 3.94LNRR297 pKa = 11.84KK298 pKa = 9.4RR299 pKa = 11.84EE300 pKa = 3.9ALGRR304 pKa = 11.84LKK306 pKa = 10.51RR307 pKa = 11.84GKK309 pKa = 10.13EE310 pKa = 3.78PFIEE314 pKa = 4.82TIKK317 pKa = 9.87KK318 pKa = 7.94TPEE321 pKa = 4.02DD322 pKa = 3.68YY323 pKa = 10.59AVKK326 pKa = 10.13VIKK329 pKa = 10.48SIMRR333 pKa = 11.84LPCSRR338 pKa = 11.84KK339 pKa = 8.16HH340 pKa = 6.94DD341 pKa = 3.87YY342 pKa = 10.78FGTEE346 pKa = 3.98MIVSKK351 pKa = 10.49KK352 pKa = 10.58GPNTEE357 pKa = 4.34VVTHH361 pKa = 6.53PSGIQWRR368 pKa = 11.84EE369 pKa = 3.67YY370 pKa = 9.68IDD372 pKa = 3.38EE373 pKa = 4.03EE374 pKa = 4.54KK375 pKa = 10.43IYY377 pKa = 10.31FRR379 pKa = 11.84HH380 pKa = 5.94LEE382 pKa = 3.87CRR384 pKa = 11.84GWFTHH389 pKa = 6.21NRR391 pKa = 11.84ATLTEE396 pKa = 4.04ITFSITDD403 pKa = 4.5RR404 pKa = 11.84ITFEE408 pKa = 3.96NLLVNIANYY417 pKa = 9.48IRR419 pKa = 11.84YY420 pKa = 9.18DD421 pKa = 3.08WVYY424 pKa = 11.04RR425 pKa = 11.84PGKK428 pKa = 9.62SIKK431 pKa = 8.38EE432 pKa = 3.95LRR434 pKa = 11.84DD435 pKa = 3.27LTMLEE440 pKa = 4.4VKK442 pKa = 10.46KK443 pKa = 10.51EE444 pKa = 3.74PWKK447 pKa = 10.79ILGTNKK453 pKa = 9.99RR454 pKa = 11.84VWEE457 pKa = 4.39EE458 pKa = 3.68YY459 pKa = 8.68WRR461 pKa = 11.84HH462 pKa = 6.36IEE464 pKa = 4.91DD465 pKa = 3.65SLITITDD472 pKa = 3.15EE473 pKa = 3.9MKK475 pKa = 10.76NYY477 pKa = 8.75LTFEE481 pKa = 4.14SKK483 pKa = 10.83VNVRR487 pKa = 11.84CVYY490 pKa = 9.12KK491 pKa = 10.43TEE493 pKa = 4.11IPEE496 pKa = 4.05EE497 pKa = 4.47TEE499 pKa = 3.25VHH501 pKa = 5.81YY502 pKa = 9.93EE503 pKa = 3.83VRR505 pKa = 11.84PDD507 pKa = 3.45GSLEE511 pKa = 4.21EE512 pKa = 4.06IDD514 pKa = 3.82GNSRR518 pKa = 11.84IPYY521 pKa = 9.74IEE523 pKa = 4.58IKK525 pKa = 9.34TAGIPHH531 pKa = 7.7LDD533 pKa = 3.6MTWNSNDD540 pKa = 3.24MYY542 pKa = 10.31IIPLLHH548 pKa = 6.35PQISMMRR555 pKa = 11.84TIEE558 pKa = 4.01FHH560 pKa = 6.78LLRR563 pKa = 11.84PNLEE567 pKa = 3.75EE568 pKa = 7.73DD569 pKa = 2.9IMKK572 pKa = 10.92NNFSTHH578 pKa = 5.78NAHH581 pKa = 6.55CPYY584 pKa = 9.42WLEE587 pKa = 4.14AASMAGFCNQARR599 pKa = 11.84MMLYY603 pKa = 10.71GAMFKK608 pKa = 10.84LKK610 pKa = 9.43TIPKK614 pKa = 9.36IFLAYY619 pKa = 9.81LYY621 pKa = 9.91CFSGYY626 pKa = 9.41PRR628 pKa = 11.84AHH630 pKa = 5.76TKK632 pKa = 9.62YY633 pKa = 10.57NEE635 pKa = 4.08RR636 pKa = 11.84ARR638 pKa = 11.84MKK640 pKa = 10.34TGGFLDD646 pKa = 5.79LYY648 pKa = 11.11AKK650 pKa = 10.48DD651 pKa = 3.73GNFRR655 pKa = 11.84RR656 pKa = 11.84DD657 pKa = 3.35PEE659 pKa = 4.26TGKK662 pKa = 9.59WLIFDD667 pKa = 4.35KK668 pKa = 10.76EE669 pKa = 4.43WVGVVTDD676 pKa = 4.33MGPEE680 pKa = 4.15PEE682 pKa = 4.03LLAYY686 pKa = 8.78TFLNGFRR693 pKa = 11.84LEE695 pKa = 4.29SVKK698 pKa = 10.27KK699 pKa = 10.46RR700 pKa = 11.84KK701 pKa = 9.42GDD703 pKa = 3.26HH704 pKa = 6.1GLANLRR710 pKa = 11.84EE711 pKa = 4.14GMEE714 pKa = 4.13ALKK717 pKa = 10.37EE718 pKa = 4.15KK719 pKa = 10.56QDD721 pKa = 3.37GEE723 pKa = 4.38MIYY726 pKa = 10.94VRR728 pKa = 11.84IGGDD732 pKa = 3.12VKK734 pKa = 11.19LLVRR738 pKa = 11.84DD739 pKa = 3.58RR740 pKa = 11.84TTDD743 pKa = 2.87AKK745 pKa = 10.51RR746 pKa = 11.84ALAIKK751 pKa = 10.2RR752 pKa = 11.84GRR754 pKa = 11.84EE755 pKa = 3.36RR756 pKa = 11.84VLEE759 pKa = 4.17DD760 pKa = 3.93RR761 pKa = 11.84EE762 pKa = 4.35LEE764 pKa = 4.25LMSSLQPKK772 pKa = 8.83RR773 pKa = 11.84AKK775 pKa = 10.5KK776 pKa = 9.39EE777 pKa = 3.74

Molecular weight:
90.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3767

128

784

538.1

61.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.053 ± 0.405

1.752 ± 0.339

5.256 ± 0.38

8.097 ± 0.749

3.955 ± 0.32

5.946 ± 0.338

2.177 ± 0.194

5.946 ± 0.328

7.008 ± 0.321

8.866 ± 0.436

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.318 ± 0.351

4.831 ± 0.406

4.088 ± 0.247

3.132 ± 0.205

6.291 ± 0.759

6.822 ± 0.547

5.177 ± 0.473

6.053 ± 0.235

1.805 ± 0.247

3.424 ± 0.314

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski