Pogonomyrmex barbatus (red harvester ant)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea; Fo

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13753 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I9WS05|A0A6I9WS05_9HYME ankyrin repeat domain-containing protein 40-like OS=Pogonomyrmex barbatus OX=144034 GN=LOC105433216 PE=4 SV=1
MM1 pKa = 7.35NNNRR5 pKa = 11.84STNDD9 pKa = 3.09TSDD12 pKa = 3.96DD13 pKa = 3.74NEE15 pKa = 4.3EE16 pKa = 4.32NNTIDD21 pKa = 4.87DD22 pKa = 3.88MNMNSDD28 pKa = 4.1RR29 pKa = 11.84LDD31 pKa = 3.5NDD33 pKa = 2.88ISDD36 pKa = 4.72DD37 pKa = 3.82NEE39 pKa = 3.81EE40 pKa = 3.92NYY42 pKa = 10.66EE43 pKa = 3.98MNEE46 pKa = 4.14NVNTATNSNNALEE59 pKa = 4.13QLRR62 pKa = 11.84EE63 pKa = 3.96VLYY66 pKa = 10.52EE67 pKa = 4.33DD68 pKa = 4.83CDD70 pKa = 3.86LTKK73 pKa = 10.76EE74 pKa = 3.99EE75 pKa = 4.46SEE77 pKa = 4.06ILIMSLALRR86 pKa = 11.84HH87 pKa = 6.12HH88 pKa = 6.35FTDD91 pKa = 4.27AALQDD96 pKa = 4.53FIKK99 pKa = 11.18VIDD102 pKa = 4.56CYY104 pKa = 11.42ASAMYY109 pKa = 10.86SNIYY113 pKa = 9.31FF114 pKa = 3.91

Molecular weight: 13.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I9WIQ2|A0A6I9WIQ2_9HYME potassium voltage-gated channel protein eag isoform X1 OS=Pogonomyrmex barbatus OX=144034 GN=LOC105430841 PE=4 SV=1
MM1 pKa = 7.16YY2 pKa = 10.44NPVVRR7 pKa = 11.84CLTQRR12 pKa = 11.84SLKK15 pKa = 10.44RR16 pKa = 11.84PNRR19 pKa = 11.84CIFANIEE26 pKa = 3.92KK27 pKa = 10.27RR28 pKa = 11.84KK29 pKa = 10.36VSLFSDD35 pKa = 4.15WFCEE39 pKa = 3.56RR40 pKa = 11.84HH41 pKa = 4.94AFARR45 pKa = 11.84ASLTLTPGTARR56 pKa = 11.84AGKK59 pKa = 8.87VRR61 pKa = 11.84SYY63 pKa = 10.71SSRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84SLGWIPSRR76 pKa = 11.84ISDD79 pKa = 5.01LISDD83 pKa = 4.4LRR85 pKa = 11.84VSILSGDD92 pKa = 3.51SGRR95 pKa = 11.84FTFVTVPTCNTGLFLSSEE113 pKa = 4.58VIGHH117 pKa = 6.79GICEE121 pKa = 4.23YY122 pKa = 10.75VRR124 pKa = 11.84EE125 pKa = 4.24KK126 pKa = 11.2

Molecular weight: 14.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9843

3910

13753

8082949

53

16579

587.7

66.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.203 ± 0.016

1.926 ± 0.016

5.541 ± 0.014

6.744 ± 0.026

3.464 ± 0.013

5.457 ± 0.026

2.533 ± 0.01

5.897 ± 0.018

6.224 ± 0.025

8.955 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.009

5.131 ± 0.015

5.079 ± 0.02

4.434 ± 0.023

5.686 ± 0.018

8.226 ± 0.027

6.034 ± 0.016

5.892 ± 0.015

1.064 ± 0.006

3.131 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski