Escherichia phage BA14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Berlinvirus; Escherichia virus BA14

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3VCQ8|B3VCQ8_9CAUD Major capsid protein OS=Escherichia phage BA14 OX=532074 GN=10A PE=3 SV=1
MM1 pKa = 7.95SGEE4 pKa = 4.31SNADD8 pKa = 3.13VYY10 pKa = 11.74ASFGVNSAVVGGSTPTDD27 pKa = 3.38HH28 pKa = 6.29EE29 pKa = 4.23QAMLEE34 pKa = 4.14LDD36 pKa = 3.56VAARR40 pKa = 11.84DD41 pKa = 3.57GDD43 pKa = 4.03DD44 pKa = 4.66AIEE47 pKa = 4.3LVGNDD52 pKa = 4.4DD53 pKa = 4.39PYY55 pKa = 11.87GNPDD59 pKa = 3.38PFGEE63 pKa = 3.98EE64 pKa = 3.92DD65 pKa = 3.44EE66 pKa = 4.45NRR68 pKa = 11.84TEE70 pKa = 4.6IRR72 pKa = 11.84ISTDD76 pKa = 3.1GEE78 pKa = 4.08PEE80 pKa = 3.82EE81 pKa = 4.47TEE83 pKa = 4.1EE84 pKa = 5.25GEE86 pKa = 3.8IDD88 pKa = 3.73YY89 pKa = 11.46SEE91 pKa = 4.39GDD93 pKa = 3.52EE94 pKa = 4.26EE95 pKa = 4.9GSEE98 pKa = 4.17EE99 pKa = 4.23FEE101 pKa = 4.44PVGDD105 pKa = 4.77VPDD108 pKa = 4.19EE109 pKa = 4.26LNEE112 pKa = 4.12ASSQLEE118 pKa = 3.72EE119 pKa = 4.54HH120 pKa = 6.31EE121 pKa = 4.7AGFEE125 pKa = 4.23EE126 pKa = 5.03MVNQAAEE133 pKa = 4.17RR134 pKa = 11.84GLSEE138 pKa = 3.98EE139 pKa = 4.34TIVRR143 pKa = 11.84IQQEE147 pKa = 4.23YY148 pKa = 10.7GEE150 pKa = 5.27DD151 pKa = 3.82GLSEE155 pKa = 3.96EE156 pKa = 5.07SYY158 pKa = 11.48EE159 pKa = 4.2EE160 pKa = 3.99LAKK163 pKa = 10.69AGYY166 pKa = 9.57SKK168 pKa = 11.18SFVDD172 pKa = 3.98SYY174 pKa = 11.69IRR176 pKa = 11.84GQEE179 pKa = 3.97ALVEE183 pKa = 4.18QYY185 pKa = 10.01VQSVMEE191 pKa = 4.15YY192 pKa = 10.75AGGEE196 pKa = 4.05AQFQAIYY203 pKa = 10.83NHH205 pKa = 6.79LEE207 pKa = 3.8VSNPDD212 pKa = 3.37AAEE215 pKa = 4.13SLVSALEE222 pKa = 3.86NRR224 pKa = 11.84DD225 pKa = 3.35LATVKK230 pKa = 10.77AIVNLAGASRR240 pKa = 11.84AKK242 pKa = 10.23TFGKK246 pKa = 9.34PAARR250 pKa = 11.84SISKK254 pKa = 9.91RR255 pKa = 11.84AVPAAPVRR263 pKa = 11.84AKK265 pKa = 10.59QSGFEE270 pKa = 4.22SQAEE274 pKa = 4.27MIAAMSDD281 pKa = 2.82PRR283 pKa = 11.84YY284 pKa = 10.16RR285 pKa = 11.84SDD287 pKa = 3.1SKK289 pKa = 10.95FRR291 pKa = 11.84AEE293 pKa = 4.32VEE295 pKa = 3.99RR296 pKa = 11.84KK297 pKa = 8.8MMYY300 pKa = 10.8SNFF303 pKa = 3.58

Molecular weight: 33.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3VCM5|B3VCM5_9CAUD Gp0.7 OS=Escherichia phage BA14 OX=532074 GN=AS7_0003 PE=4 SV=1
MM1 pKa = 7.07VALFVITMYY10 pKa = 11.5ALIGAYY16 pKa = 8.96FLRR19 pKa = 11.84DD20 pKa = 3.39FRR22 pKa = 11.84KK23 pKa = 9.71GLKK26 pKa = 7.69VHH28 pKa = 6.82KK29 pKa = 10.65ANFSHH34 pKa = 7.0LKK36 pKa = 10.44YY37 pKa = 10.74GFLPRR42 pKa = 11.84FTVRR46 pKa = 11.84EE47 pKa = 3.72RR48 pKa = 11.84NGRR51 pKa = 11.84FKK53 pKa = 11.15ANKK56 pKa = 9.23VGIFYY61 pKa = 10.82VATHH65 pKa = 6.59

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12261

26

1315

235.8

26.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.061 ± 0.528

1.134 ± 0.214

6.06 ± 0.247

7.071 ± 0.414

3.589 ± 0.188

7.716 ± 0.332

1.949 ± 0.172

4.771 ± 0.22

6.957 ± 0.296

8.034 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.107 ± 0.231

4.624 ± 0.264

3.532 ± 0.19

3.752 ± 0.216

5.505 ± 0.237

5.766 ± 0.292

5.595 ± 0.195

6.908 ± 0.359

1.59 ± 0.192

3.279 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski