Ruminococcus bromii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5JCR0|A0A1G5JCR0_9FIRM Ribosomal RNA large subunit methyltransferase H OS=Ruminococcus bromii OX=40518 GN=rlmH PE=3 SV=1
MM1 pKa = 7.29KK2 pKa = 10.49LIYY5 pKa = 9.26ATYY8 pKa = 9.83NQYY11 pKa = 11.07CNSIDD16 pKa = 2.95ITTFDD21 pKa = 4.67NILLRR26 pKa = 11.84IDD28 pKa = 3.49CSKK31 pKa = 11.53AEE33 pKa = 5.03DD34 pKa = 4.0GLKK37 pKa = 8.73ITPNSQCALNALAIDD52 pKa = 4.04EE53 pKa = 4.32PMEE56 pKa = 4.03YY57 pKa = 10.74ARR59 pKa = 11.84LVLDD63 pKa = 4.5GEE65 pKa = 4.31MQAWIDD71 pKa = 3.86AEE73 pKa = 4.41DD74 pKa = 3.82SLEE77 pKa = 3.99VWW79 pKa = 3.66

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5FVK1|A0A1G5FVK1_9FIRM Helix-turn-helix OS=Ruminococcus bromii OX=40518 GN=SAMN02910441_01458 PE=4 SV=1
MM1 pKa = 7.36QSAQIIIINGTLRR14 pKa = 11.84EE15 pKa = 3.97IGAFLLLSRR24 pKa = 11.84RR25 pKa = 11.84AHH27 pKa = 7.06LLTTCEE33 pKa = 4.18AGGGIPPAGQARR45 pKa = 11.84ALFTNCLSASSPEE58 pKa = 4.3GAGNAAGWHH67 pKa = 5.38SLCSRR72 pKa = 11.84HH73 pKa = 6.28FCLCRR78 pKa = 11.84DD79 pKa = 2.92WCLPHH84 pKa = 7.27QSGRR88 pKa = 11.84CSARR92 pKa = 11.84LLPAKK97 pKa = 9.29TCHH100 pKa = 5.57RR101 pKa = 11.84QLYY104 pKa = 9.41RR105 pKa = 11.84SYY107 pKa = 11.44GRR109 pKa = 11.84LAPPIFFLRR118 pKa = 11.84KK119 pKa = 8.79RR120 pKa = 11.84NKK122 pKa = 8.97NQEE125 pKa = 3.98RR126 pKa = 11.84KK127 pKa = 10.41DD128 pKa = 3.81NEE130 pKa = 4.32NKK132 pKa = 8.75WRR134 pKa = 11.84SIIWKK139 pKa = 9.0QRR141 pKa = 11.84LL142 pKa = 3.24

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2484

0

2484

738748

15

6641

297.4

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.875 ± 0.052

1.719 ± 0.023

6.173 ± 0.042

6.685 ± 0.05

4.415 ± 0.043

6.424 ± 0.045

1.508 ± 0.02

7.929 ± 0.051

7.789 ± 0.042

8.315 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.029

5.726 ± 0.053

3.085 ± 0.028

2.862 ± 0.028

3.733 ± 0.042

6.825 ± 0.056

5.607 ± 0.049

6.689 ± 0.042

0.786 ± 0.017

4.302 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski