Beihai narna-like virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIB8|A0A1L3KIB8_9VIRU Uncharacterized protein OS=Beihai narna-like virus 10 OX=1922437 PE=4 SV=1
MM1 pKa = 7.51NFRR4 pKa = 11.84LEE6 pKa = 4.41DD7 pKa = 3.47QLVMYY12 pKa = 7.76RR13 pKa = 11.84TAAQWPPEE21 pKa = 3.97KK22 pKa = 10.08FIKK25 pKa = 8.98FAKK28 pKa = 8.76YY29 pKa = 4.53TTAWPMAKK37 pKa = 9.78FLEE40 pKa = 4.55NDD42 pKa = 3.58LPAVPDD48 pKa = 4.22GFQGNPLWSGAIKK61 pKa = 10.55RR62 pKa = 11.84FLKK65 pKa = 9.72TRR67 pKa = 11.84IVARR71 pKa = 11.84SPRR74 pKa = 11.84NGRR77 pKa = 11.84LFFGILQGVKK87 pKa = 9.68KK88 pKa = 10.57ACAQVPEE95 pKa = 4.4SFVMEE100 pKa = 4.69AYY102 pKa = 10.1VKK104 pKa = 10.7HH105 pKa = 6.78RR106 pKa = 11.84DD107 pKa = 3.65ALSGEE112 pKa = 4.15PRR114 pKa = 11.84GVDD117 pKa = 3.16PGQMSFYY124 pKa = 10.1YY125 pKa = 10.36RR126 pKa = 11.84EE127 pKa = 3.74FFKK130 pKa = 10.63RR131 pKa = 11.84FRR133 pKa = 11.84PRR135 pKa = 11.84KK136 pKa = 7.45PKK138 pKa = 10.4LYY140 pKa = 9.31EE141 pKa = 4.05ASNSASFEE149 pKa = 4.46TVRR152 pKa = 11.84SMGGARR158 pKa = 11.84GWIQSNHH165 pKa = 6.58DD166 pKa = 4.01LVDD169 pKa = 4.57DD170 pKa = 5.36DD171 pKa = 5.28GLIGMFEE178 pKa = 4.0TRR180 pKa = 11.84PGKK183 pKa = 10.48VEE185 pKa = 4.22TVKK188 pKa = 11.04GPALEE193 pKa = 4.15PTFDD197 pKa = 3.88EE198 pKa = 5.09LVDD201 pKa = 4.21LALEE205 pKa = 4.27EE206 pKa = 4.45PKK208 pKa = 10.37SVRR211 pKa = 11.84VGAVLEE217 pKa = 4.05PLKK220 pKa = 11.06VRR222 pKa = 11.84LITKK226 pKa = 10.13GNTLRR231 pKa = 11.84YY232 pKa = 8.16WLSRR236 pKa = 11.84DD237 pKa = 3.54CQKK240 pKa = 10.81QLWSYY245 pKa = 8.2LQKK248 pKa = 10.58YY249 pKa = 9.29DD250 pKa = 3.87QFALTGRR257 pKa = 11.84PLMAYY262 pKa = 9.04DD263 pKa = 3.39LHH265 pKa = 7.43GILIRR270 pKa = 11.84EE271 pKa = 4.38EE272 pKa = 3.95KK273 pKa = 10.76LGLKK277 pKa = 9.34FDD279 pKa = 3.65KK280 pKa = 10.14WVSGDD285 pKa = 3.39YY286 pKa = 10.71AAATDD291 pKa = 4.01TLDD294 pKa = 4.49LRR296 pKa = 11.84HH297 pKa = 6.01TKK299 pKa = 10.29AAFEE303 pKa = 4.06EE304 pKa = 4.15ALRR307 pKa = 11.84MCMFSYY313 pKa = 10.26SPKK316 pKa = 8.82YY317 pKa = 9.8QDD319 pKa = 3.12VLRR322 pKa = 11.84SVLYY326 pKa = 9.47EE327 pKa = 3.95QEE329 pKa = 3.21IHH331 pKa = 6.04YY332 pKa = 10.6PEE334 pKa = 4.86GAVKK338 pKa = 10.2KK339 pKa = 10.34CAGKK343 pKa = 10.22LDD345 pKa = 3.92PVMQRR350 pKa = 11.84TGQLMGSTLSFPILCAINLCAYY372 pKa = 7.73WAALEE377 pKa = 4.12EE378 pKa = 4.31LTGRR382 pKa = 11.84EE383 pKa = 4.01FDD385 pKa = 3.75VHH387 pKa = 6.84DD388 pKa = 5.31LPVLVNGDD396 pKa = 4.49DD397 pKa = 3.29ILFRR401 pKa = 11.84TDD403 pKa = 3.58DD404 pKa = 3.56RR405 pKa = 11.84LYY407 pKa = 10.75DD408 pKa = 2.82IWLRR412 pKa = 11.84KK413 pKa = 7.0TKK415 pKa = 10.3EE416 pKa = 3.73VGFEE420 pKa = 3.76LSLGKK425 pKa = 10.42NYY427 pKa = 9.5VHH429 pKa = 7.25PNYY432 pKa = 9.83LTVNSQLYY440 pKa = 8.53YY441 pKa = 10.86FKK443 pKa = 10.5NTTFRR448 pKa = 11.84GKK450 pKa = 10.1KK451 pKa = 9.12KK452 pKa = 10.82SLFIPLGYY460 pKa = 10.26LRR462 pKa = 11.84AGLLTGQSKK471 pKa = 8.2ITGRR475 pKa = 11.84QEE477 pKa = 3.79AKK479 pKa = 10.32AAPLWDD485 pKa = 3.69YY486 pKa = 10.76FNKK489 pKa = 8.09VTRR492 pKa = 11.84EE493 pKa = 3.78ALDD496 pKa = 4.11PIRR499 pKa = 11.84AKK501 pKa = 10.54RR502 pKa = 11.84RR503 pKa = 11.84FISYY507 pKa = 10.31HH508 pKa = 6.44KK509 pKa = 9.77DD510 pKa = 2.91TIVRR514 pKa = 11.84LTQKK518 pKa = 10.68GKK520 pKa = 9.21WNLFASPMKK529 pKa = 10.46GGLGFEE535 pKa = 4.19PVEE538 pKa = 4.09GDD540 pKa = 3.42KK541 pKa = 11.36LRR543 pKa = 11.84FTPFQRR549 pKa = 11.84RR550 pKa = 11.84WADD553 pKa = 3.37FMDD556 pKa = 3.27WKK558 pKa = 10.79LRR560 pKa = 11.84NDD562 pKa = 3.8PDD564 pKa = 3.65NFGTISLIQSRR575 pKa = 11.84SSKK578 pKa = 10.48DD579 pKa = 3.42GPRR582 pKa = 11.84VLHH585 pKa = 6.22NPQLTVQPRR594 pKa = 11.84YY595 pKa = 9.81GPYY598 pKa = 9.7EE599 pKa = 4.02EE600 pKa = 4.84GVVEE604 pKa = 5.15IKK606 pKa = 10.01DD607 pKa = 3.52TTIPLQILAARR618 pKa = 11.84LEE620 pKa = 4.37INDD623 pKa = 3.93AEE625 pKa = 4.37DD626 pKa = 3.22MKK628 pKa = 11.26SALRR632 pKa = 11.84VKK634 pKa = 10.28FPKK637 pKa = 10.33KK638 pKa = 8.64EE639 pKa = 3.98TLKK642 pKa = 10.62EE643 pKa = 3.78FRR645 pKa = 11.84SRR647 pKa = 11.84NWRR650 pKa = 11.84QLKK653 pKa = 10.61GSLTCDD659 pKa = 2.88RR660 pKa = 11.84FRR662 pKa = 11.84LMEE665 pKa = 4.67KK666 pKa = 9.81RR667 pKa = 11.84SEE669 pKa = 4.17MNSKK673 pKa = 9.84VLSALEE679 pKa = 3.87QMDD682 pKa = 4.04GVTEE686 pKa = 4.12LLQEE690 pKa = 4.27YY691 pKa = 10.35KK692 pKa = 9.74STGTFNSLSLEE703 pKa = 3.84IMNDD707 pKa = 2.88IVEE710 pKa = 5.11GICPSCSAAEE720 pKa = 3.98KK721 pKa = 10.4KK722 pKa = 10.38KK723 pKa = 9.81YY724 pKa = 9.91LSHH727 pKa = 7.23RR728 pKa = 11.84EE729 pKa = 3.79YY730 pKa = 11.38SEE732 pKa = 3.86

Molecular weight: 84.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIB8|A0A1L3KIB8_9VIRU Uncharacterized protein OS=Beihai narna-like virus 10 OX=1922437 PE=4 SV=1
MM1 pKa = 7.48SNNTRR6 pKa = 11.84SPIKK10 pKa = 10.09KK11 pKa = 9.77KK12 pKa = 10.55NGGSRR17 pKa = 11.84GRR19 pKa = 11.84RR20 pKa = 11.84PIRR23 pKa = 11.84GARR26 pKa = 11.84LAPFRR31 pKa = 11.84GLAQSMALVPARR43 pKa = 11.84AFGGASSSHH52 pKa = 5.88SVLKK56 pKa = 10.99GLDD59 pKa = 3.36AFDD62 pKa = 3.92EE63 pKa = 4.4HH64 pKa = 7.54HH65 pKa = 6.61VALPRR70 pKa = 11.84AVANYY75 pKa = 8.44TVIRR79 pKa = 11.84TTQVLNQADD88 pKa = 3.65ATLSVFGPLAITDD101 pKa = 4.3FQAPGYY107 pKa = 7.38GTQWSNGFCVQSVDD121 pKa = 3.56PQQSINVANNAVLKK135 pKa = 9.23TFSFMGGNNTSWNGVRR151 pKa = 11.84MTPAAFTIKK160 pKa = 10.36VMNPEE165 pKa = 4.03ALQTTRR171 pKa = 11.84GAVYY175 pKa = 10.25IGRR178 pKa = 11.84AKK180 pKa = 10.18QQLNLGGNVRR190 pKa = 11.84TWNDD194 pKa = 3.0LANALVSYY202 pKa = 9.01SAPEE206 pKa = 3.5IVAAGRR212 pKa = 11.84LALRR216 pKa = 11.84GVKK219 pKa = 9.71VDD221 pKa = 3.63ALPYY225 pKa = 10.88DD226 pKa = 3.87MQSLSDD232 pKa = 3.79FRR234 pKa = 11.84SGKK237 pKa = 9.94LLQDD241 pKa = 4.13NVFTWGDD248 pKa = 3.21QSTDD252 pKa = 3.51FDD254 pKa = 3.81GFAPIFVYY262 pKa = 10.84NPDD265 pKa = 3.45QVKK268 pKa = 10.01LQILVCCEE276 pKa = 3.01WRR278 pKa = 11.84VRR280 pKa = 11.84FDD282 pKa = 3.89PEE284 pKa = 3.57NPAYY288 pKa = 10.2ASHH291 pKa = 6.96TYY293 pKa = 9.88HH294 pKa = 7.26RR295 pKa = 11.84PTSLAYY301 pKa = 7.96WDD303 pKa = 3.53RR304 pKa = 11.84VQRR307 pKa = 11.84IGNSVGNGVFDD318 pKa = 5.97LIEE321 pKa = 4.1KK322 pKa = 9.57AAPAVIGNMAQRR334 pKa = 11.84AVGRR338 pKa = 11.84IAQGVASSSVPLLMNAAVV356 pKa = 3.44

Molecular weight: 38.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1088

356

732

544.0

61.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.18 ± 1.969

1.103 ± 0.142

5.423 ± 0.352

5.055 ± 1.834

5.055 ± 0.305

7.169 ± 0.685

1.379 ± 0.014

3.86 ± 0.039

6.342 ± 1.77

9.835 ± 1.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.128

4.32 ± 1.318

5.239 ± 0.053

4.228 ± 0.604

7.169 ± 0.233

6.25 ± 0.573

5.055 ± 0.001

6.526 ± 1.341

1.838 ± 0.083

3.493 ± 0.525
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski