Lachnospiraceae bacterium 5_1_57FAA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3365 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7KM73|F7KM73_9FIRM Y1_Tnp domain-containing protein (Fragment) OS=Lachnospiraceae bacterium 5_1_57FAA OX=658085 GN=HMPREF0993_00066 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 4.92FEE4 pKa = 4.24QDD6 pKa = 3.21YY7 pKa = 10.41IMRR10 pKa = 11.84MIQDD14 pKa = 3.55IVRR17 pKa = 11.84FMMQLITGRR26 pKa = 11.84QQFQYY31 pKa = 11.17DD32 pKa = 3.5FADD35 pKa = 3.84FTGNMTGNDD44 pKa = 3.01HH45 pKa = 7.04FARR48 pKa = 11.84LIALADD54 pKa = 3.35AGMINSAEE62 pKa = 3.72NMMYY66 pKa = 10.81EE67 pKa = 5.05DD68 pKa = 5.76LDD70 pKa = 4.7PEE72 pKa = 4.37DD73 pKa = 5.11KK74 pKa = 10.55EE75 pKa = 4.34YY76 pKa = 11.54LMLGLAFYY84 pKa = 10.56SHH86 pKa = 6.83INEE89 pKa = 4.18YY90 pKa = 11.12SDD92 pKa = 5.75DD93 pKa = 3.96FLTAADD99 pKa = 3.81YY100 pKa = 11.27SRR102 pKa = 11.84AEE104 pKa = 3.84IQEE107 pKa = 4.53GIRR110 pKa = 11.84SLLNEE115 pKa = 4.0YY116 pKa = 10.66GITGLDD122 pKa = 3.69AIYY125 pKa = 10.65EE126 pKa = 4.29SLDD129 pKa = 3.24

Molecular weight: 14.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7KMV3|F7KMV3_9FIRM Histidine kinase OS=Lachnospiraceae bacterium 5_1_57FAA OX=658085 GN=HMPREF0993_00295 PE=4 SV=1
MM1 pKa = 7.76AYY3 pKa = 10.38DD4 pKa = 3.32KK5 pKa = 11.38GNRR8 pKa = 11.84PEE10 pKa = 4.72GGFKK14 pKa = 10.43RR15 pKa = 11.84RR16 pKa = 11.84GGGRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 9.23KK24 pKa = 10.13VCVFCGKK31 pKa = 9.9EE32 pKa = 3.91NNEE35 pKa = 3.46IDD37 pKa = 4.06YY38 pKa = 11.26KK39 pKa = 11.27DD40 pKa = 3.48VAKK43 pKa = 10.34LRR45 pKa = 11.84KK46 pKa = 9.47YY47 pKa = 9.7ISEE50 pKa = 4.06RR51 pKa = 11.84GKK53 pKa = 9.99ILPRR57 pKa = 11.84RR58 pKa = 11.84ITGNCAKK65 pKa = 9.81HH66 pKa = 5.44QRR68 pKa = 11.84ALTVAIKK75 pKa = 10.29RR76 pKa = 11.84ARR78 pKa = 11.84HH79 pKa = 4.72VALMPYY85 pKa = 9.71VQDD88 pKa = 3.38

Molecular weight: 10.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3365

0

3365

986099

29

2451

293.0

32.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.701 ± 0.051

1.539 ± 0.02

5.633 ± 0.033

7.781 ± 0.047

3.869 ± 0.029

7.414 ± 0.041

1.744 ± 0.019

7.517 ± 0.038

6.915 ± 0.039

8.886 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.297 ± 0.021

3.962 ± 0.028

3.311 ± 0.022

3.253 ± 0.023

4.616 ± 0.04

5.525 ± 0.032

5.161 ± 0.032

6.871 ± 0.031

0.897 ± 0.015

4.109 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski