Wohlfahrtiimonas chitiniclastica SH04

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Wohlfahrtiimonas; Wohlfahrtiimonas chitiniclastica

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8XUV4|L8XUV4_9GAMM Uncharacterized protein OS=Wohlfahrtiimonas chitiniclastica SH04 OX=1261130 GN=F387_01496 PE=4 SV=1
MM1 pKa = 7.55TYY3 pKa = 10.67KK4 pKa = 9.54KK5 pKa = 8.29TALSIASLLILGTFSYY21 pKa = 10.61AQQTPTEE28 pKa = 4.44LEE30 pKa = 3.97PNGIIAYY37 pKa = 9.15FAEE40 pKa = 5.6DD41 pKa = 3.51GTLLPSRR48 pKa = 11.84EE49 pKa = 3.43GSEE52 pKa = 4.5YY53 pKa = 10.03YY54 pKa = 10.27RR55 pKa = 11.84HH56 pKa = 6.13FKK58 pKa = 10.89DD59 pKa = 4.05VVSGCYY65 pKa = 9.46LVQDD69 pKa = 5.43FYY71 pKa = 11.99SQNDD75 pKa = 3.44QKK77 pKa = 10.24QTDD80 pKa = 5.09PICFMDD86 pKa = 4.54PGEE89 pKa = 4.13LQSWFPQSLYY99 pKa = 11.12GPLTFWHH106 pKa = 6.98KK107 pKa = 11.04DD108 pKa = 3.01GTKK111 pKa = 9.96AQSGYY116 pKa = 9.12SQQDD120 pKa = 3.05GGGNGTWTTWDD131 pKa = 3.23QDD133 pKa = 3.85GNASDD138 pKa = 5.29YY139 pKa = 10.93EE140 pKa = 4.26LQNGEE145 pKa = 4.91LIVSYY150 pKa = 10.64FDD152 pKa = 3.94EE153 pKa = 4.65NGNRR157 pKa = 11.84QPNAVDD163 pKa = 3.02GGTFRR168 pKa = 11.84TILSYY173 pKa = 11.24DD174 pKa = 3.5EE175 pKa = 5.39SSDD178 pKa = 3.61SFLVADD184 pKa = 4.45YY185 pKa = 8.17FTDD188 pKa = 3.07TRR190 pKa = 11.84TRR192 pKa = 11.84QMDD195 pKa = 3.58PVVIARR201 pKa = 11.84EE202 pKa = 3.9DD203 pKa = 3.49LLRR206 pKa = 11.84WNIEE210 pKa = 3.93NRR212 pKa = 11.84EE213 pKa = 4.01GTYY216 pKa = 10.6VYY218 pKa = 10.9YY219 pKa = 11.12NEE221 pKa = 4.31AQEE224 pKa = 3.83ITAQEE229 pKa = 4.66EE230 pKa = 4.58YY231 pKa = 11.16AQGKK235 pKa = 9.77LNGTVTFWYY244 pKa = 10.32QDD246 pKa = 3.06TGLPAQKK253 pKa = 9.62MEE255 pKa = 4.98EE256 pKa = 4.15INFKK260 pKa = 10.72DD261 pKa = 3.82GQQEE265 pKa = 4.0GLYY268 pKa = 10.31VSWYY272 pKa = 8.59PNGYY276 pKa = 7.27PQYY279 pKa = 10.23RR280 pKa = 11.84INFTDD285 pKa = 3.23MGIDD289 pKa = 5.07SVACWDD295 pKa = 3.7EE296 pKa = 4.15NFVAGTEE303 pKa = 4.14DD304 pKa = 3.13EE305 pKa = 4.49CMARR309 pKa = 11.84LTGAAEE315 pKa = 3.98AAEE318 pKa = 4.41VEE320 pKa = 4.53ANANDD325 pKa = 4.73EE326 pKa = 4.47INDD329 pKa = 4.0DD330 pKa = 4.33LDD332 pKa = 3.88VEE334 pKa = 4.71IIIPSDD340 pKa = 3.44QVVTPTVEE348 pKa = 4.09NQSDD352 pKa = 4.28LEE354 pKa = 4.24DD355 pKa = 5.86AITPDD360 pKa = 3.13ATVLFPHH367 pKa = 7.43DD368 pKa = 3.98EE369 pKa = 4.28DD370 pKa = 4.05NRR372 pKa = 11.84ILIDD376 pKa = 4.28DD377 pKa = 4.3ADD379 pKa = 3.98EE380 pKa = 5.04AFDD383 pKa = 3.96SAVDD387 pKa = 3.76TTEE390 pKa = 3.96TTPALTDD397 pKa = 3.34EE398 pKa = 4.88EE399 pKa = 4.2EE400 pKa = 4.28AADD403 pKa = 3.53IEE405 pKa = 4.5AVEE408 pKa = 4.46EE409 pKa = 4.29ADD411 pKa = 5.34DD412 pKa = 4.06VQIPNQPEE420 pKa = 4.06PEE422 pKa = 4.29TEE424 pKa = 3.98GKK426 pKa = 10.49RR427 pKa = 11.84ILRR430 pKa = 11.84SLNNILEE437 pKa = 4.28ALL439 pKa = 3.9

Molecular weight: 49.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L8XXF5|L8XXF5_9GAMM Cyclopropane-fatty-acyl-phospholipid synthase OS=Wohlfahrtiimonas chitiniclastica SH04 OX=1261130 GN=F387_01198 PE=3 SV=1
MM1 pKa = 7.69AVNQNYY7 pKa = 7.53GTGRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.42TSAARR18 pKa = 11.84VFLRR22 pKa = 11.84KK23 pKa = 8.53GTGNIVVNGKK33 pKa = 8.73PLDD36 pKa = 3.66EE37 pKa = 4.25YY38 pKa = 10.96FGRR41 pKa = 11.84KK42 pKa = 5.21TACMVVRR49 pKa = 11.84QPLEE53 pKa = 3.91LLDD56 pKa = 3.72ATEE59 pKa = 4.01QFDD62 pKa = 4.17VYY64 pKa = 9.71VTVVGGGQTGQSGAIRR80 pKa = 11.84HH81 pKa = 5.65GLTRR85 pKa = 11.84ALIEE89 pKa = 3.9YY90 pKa = 10.65DD91 pKa = 3.51EE92 pKa = 4.72VNRR95 pKa = 11.84SALRR99 pKa = 11.84KK100 pKa = 9.23AGYY103 pKa = 6.29VTRR106 pKa = 11.84DD107 pKa = 3.04ARR109 pKa = 11.84EE110 pKa = 3.89VEE112 pKa = 4.21RR113 pKa = 11.84KK114 pKa = 9.64KK115 pKa = 11.05VGLRR119 pKa = 11.84KK120 pKa = 9.46ARR122 pKa = 11.84RR123 pKa = 11.84ATQFSKK129 pKa = 10.98RR130 pKa = 3.61

Molecular weight: 14.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2004

0

2004

650783

14

4331

324.7

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.881 ± 0.061

0.893 ± 0.018

5.504 ± 0.05

6.08 ± 0.069

4.228 ± 0.041

6.735 ± 0.09

2.494 ± 0.028

7.312 ± 0.05

5.516 ± 0.046

10.33 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.031

4.364 ± 0.064

3.934 ± 0.041

4.085 ± 0.041

4.341 ± 0.048

5.771 ± 0.038

5.667 ± 0.063

6.738 ± 0.048

1.086 ± 0.02

3.215 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski