Snakehead retrovirus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; unclassified Orthoretrovirinae

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q88283|Q88283_9RETR Isoform of Q88282 Env protein OS=Snakehead retrovirus OX=40270 GN=env PE=4 SV=1
MM1 pKa = 7.15ATSFKK6 pKa = 10.34EE7 pKa = 4.13PPGCGSDD14 pKa = 3.33EE15 pKa = 5.34DD16 pKa = 4.84KK17 pKa = 11.69DD18 pKa = 3.69NDD20 pKa = 3.94TPSRR24 pKa = 11.84VPKK27 pKa = 9.62PEE29 pKa = 3.9EE30 pKa = 4.19VISLYY35 pKa = 10.81NPEE38 pKa = 3.94LFAWGGTLGDD48 pKa = 4.1YY49 pKa = 8.81VWCKK53 pKa = 6.35TTTIRR58 pKa = 11.84WGPIVMKK65 pKa = 10.62YY66 pKa = 9.47RR67 pKa = 11.84VCGHH71 pKa = 7.07LEE73 pKa = 3.74SDD75 pKa = 4.07YY76 pKa = 8.72TTSQYY81 pKa = 10.87YY82 pKa = 9.43RR83 pKa = 11.84EE84 pKa = 4.64SVNQTWIVGHH94 pKa = 5.79SGRR97 pKa = 11.84EE98 pKa = 3.9VTHH101 pKa = 6.49TCDD104 pKa = 3.76HH105 pKa = 6.51YY106 pKa = 11.31CRR108 pKa = 11.84CLNPPRR114 pKa = 11.84FCWCRR119 pKa = 11.84KK120 pKa = 7.54HH121 pKa = 6.71KK122 pKa = 10.0RR123 pKa = 11.84WHH125 pKa = 6.31PFISPLGLVHH135 pKa = 6.68WSWMEE140 pKa = 3.89KK141 pKa = 9.88FVQPVIRR148 pKa = 11.84LEE150 pKa = 3.86GWKK153 pKa = 9.72EE154 pKa = 3.75LKK156 pKa = 10.53EE157 pKa = 4.05EE158 pKa = 4.66LLTHH162 pKa = 6.73PDD164 pKa = 3.7KK165 pKa = 11.21IKK167 pKa = 8.96IWVGRR172 pKa = 11.84VHH174 pKa = 6.75GRR176 pKa = 11.84ISPDD180 pKa = 3.23LSEE183 pKa = 5.56HH184 pKa = 5.7EE185 pKa = 5.12LMVNMCAQGTLKK197 pKa = 9.4PTLLPALWW205 pKa = 3.98

Molecular weight:
23.92 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q88288|Q88288_9RETR Uncharacterized protein OS=Snakehead retrovirus OX=40270 PE=4 SV=1
MM1 pKa = 7.69WDD3 pKa = 3.31LWDD6 pKa = 4.57LVPMDD11 pKa = 4.37TEE13 pKa = 4.09QALGGSWKK21 pKa = 10.06SRR23 pKa = 11.84KK24 pKa = 5.83PTRR27 pKa = 11.84RR28 pKa = 11.84GLYY31 pKa = 8.8RR32 pKa = 11.84EE33 pKa = 3.76TGEE36 pKa = 4.22RR37 pKa = 11.84HH38 pKa = 6.63DD39 pKa = 4.09GQIFQPIRR47 pKa = 11.84NRR49 pKa = 11.84SNQVHH54 pKa = 6.52RR55 pKa = 11.84PQPPRR60 pKa = 11.84PTAPNPDD67 pKa = 3.19NQRR70 pKa = 11.84PIRR73 pKa = 11.84EE74 pKa = 4.16PRR76 pKa = 11.84PGEE79 pKa = 4.12PEE81 pKa = 4.14HH82 pKa = 8.06GDD84 pKa = 3.73FLQGASWMWQQ94 pKa = 2.92

Molecular weight:
11.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

4192

52

2017

598.9

67.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.178 ± 0.905

1.956 ± 0.236

4.771 ± 0.359

6.751 ± 0.718

2.314 ± 0.184

8.111 ± 0.249

2.767 ± 0.351

4.222 ± 0.49

7.013 ± 0.812

8.564 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.147 ± 0.115

3.793 ± 0.576

6.441 ± 0.517

5.94 ± 0.277

5.677 ± 0.502

5.248 ± 0.227

6.799 ± 0.519

5.725 ± 0.166

3.149 ± 0.207

2.409 ± 0.206

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski