Bourbon virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Thogotovirus; unclassified Thogotovirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0C5I425|A0A0C5I425_9ORTO Nucleoprotein OS=Bourbon virus OX=1618189 PE=4 SV=2
MM1 pKa = 7.41EE2 pKa = 5.48RR3 pKa = 11.84FRR5 pKa = 11.84PKK7 pKa = 10.13TISTEE12 pKa = 3.16IWQMSEE18 pKa = 5.28HH19 pKa = 4.95STEE22 pKa = 4.31WISASSPHH30 pKa = 6.12EE31 pKa = 3.98MRR33 pKa = 11.84ILEE36 pKa = 4.26CVKK39 pKa = 9.97YY40 pKa = 7.99TVCCHH45 pKa = 6.09ISDD48 pKa = 3.74MHH50 pKa = 6.57LHH52 pKa = 6.38NGLPRR57 pKa = 11.84YY58 pKa = 9.3ILLTWCPKK66 pKa = 9.98EE67 pKa = 4.3VVDD70 pKa = 5.7SVMRR74 pKa = 11.84QNQMVFARR82 pKa = 11.84PNIPDD87 pKa = 3.59MLDD90 pKa = 3.12LKK92 pKa = 10.0TKK94 pKa = 10.48LGLYY98 pKa = 9.28VKK100 pKa = 10.01IYY102 pKa = 10.24TNEE105 pKa = 4.11DD106 pKa = 3.03QPKK109 pKa = 10.32DD110 pKa = 3.61YY111 pKa = 11.26DD112 pKa = 3.72SAPNEE117 pKa = 4.11MILRR121 pKa = 11.84MTTTGRR127 pKa = 11.84FSPATLEE134 pKa = 3.99YY135 pKa = 10.5LDD137 pKa = 5.01PSQSQDD143 pKa = 2.76VRR145 pKa = 11.84NFLVTFYY152 pKa = 11.31NEE154 pKa = 4.09CLEE157 pKa = 4.4SNLYY161 pKa = 10.03EE162 pKa = 5.02RR163 pKa = 11.84IARR166 pKa = 11.84VQNEE170 pKa = 4.4EE171 pKa = 4.18PVPFAVPLTVEE182 pKa = 4.12TLLLQDD188 pKa = 4.13TDD190 pKa = 4.82LDD192 pKa = 4.87LIPNLEE198 pKa = 4.21VSGTIPPVKK207 pKa = 9.91GDD209 pKa = 3.03RR210 pKa = 11.84WSQMKK215 pKa = 8.4TLPKK219 pKa = 10.28LKK221 pKa = 9.89TPIGRR226 pKa = 11.84PAKK229 pKa = 9.39WGAWLAGKK237 pKa = 7.5EE238 pKa = 3.99ARR240 pKa = 11.84FKK242 pKa = 11.46VFDD245 pKa = 3.9EE246 pKa = 4.38STDD249 pKa = 3.45QVVAARR255 pKa = 11.84FLADD259 pKa = 3.45YY260 pKa = 9.81TDD262 pKa = 4.02TVCVRR267 pKa = 11.84EE268 pKa = 4.2SHH270 pKa = 6.08ATPKK274 pKa = 10.33TIIEE278 pKa = 4.33TVLASLRR285 pKa = 11.84AFSEE289 pKa = 4.21GRR291 pKa = 11.84PKK293 pKa = 9.56RR294 pKa = 11.84TKK296 pKa = 10.16IQEE299 pKa = 4.0DD300 pKa = 3.74LQAFGIGIKK309 pKa = 9.71KK310 pKa = 9.96RR311 pKa = 11.84KK312 pKa = 8.98KK313 pKa = 8.61QDD315 pKa = 2.99EE316 pKa = 4.22EE317 pKa = 4.26VRR319 pKa = 11.84LKK321 pKa = 11.2SSVDD325 pKa = 2.83DD326 pKa = 3.78WKK328 pKa = 10.96RR329 pKa = 11.84ASFPMEE335 pKa = 3.91EE336 pKa = 4.1ASEE339 pKa = 4.36PEE341 pKa = 4.12WVVEE345 pKa = 3.87EE346 pKa = 4.59LEE348 pKa = 4.16EE349 pKa = 4.72LEE351 pKa = 5.28RR352 pKa = 11.84PTDD355 pKa = 4.01MEE357 pKa = 4.69WLTLDD362 pKa = 4.13DD363 pKa = 5.17NYY365 pKa = 10.71IHH367 pKa = 6.96TIVDD371 pKa = 3.22EE372 pKa = 4.62HH373 pKa = 6.25ATEE376 pKa = 4.36AVNSYY381 pKa = 10.9KK382 pKa = 10.88DD383 pKa = 3.26IVTKK387 pKa = 10.55LWVSALIEE395 pKa = 4.16RR396 pKa = 11.84MQVAATRR403 pKa = 11.84AFNEE407 pKa = 3.99LHH409 pKa = 6.17TDD411 pKa = 3.46RR412 pKa = 11.84AKK414 pKa = 10.08VTTVPIITRR423 pKa = 11.84KK424 pKa = 8.09SWKK427 pKa = 7.86THH429 pKa = 5.28SLSQLWGFVIVGPHH443 pKa = 6.8HH444 pKa = 7.08IKK446 pKa = 10.43QEE448 pKa = 3.92TDD450 pKa = 3.61RR451 pKa = 11.84IPIVTVEE458 pKa = 4.96LVSEE462 pKa = 4.73DD463 pKa = 3.82NPEE466 pKa = 3.99KK467 pKa = 10.82YY468 pKa = 9.93PNHH471 pKa = 5.58SHH473 pKa = 7.63FKK475 pKa = 10.02FLYY478 pKa = 9.91HH479 pKa = 7.14KK480 pKa = 10.84GNDD483 pKa = 3.99SVGHH487 pKa = 6.32EE488 pKa = 4.19DD489 pKa = 3.29VLVRR493 pKa = 11.84VTSIAKK499 pKa = 9.62YY500 pKa = 10.63KK501 pKa = 10.73LFTFSTIRR509 pKa = 11.84RR510 pKa = 11.84VYY512 pKa = 9.91IQPCSVFSKK521 pKa = 11.1LILKK525 pKa = 10.11QSAEE529 pKa = 3.91KK530 pKa = 10.44RR531 pKa = 11.84EE532 pKa = 4.02KK533 pKa = 10.94DD534 pKa = 3.35FSLEE538 pKa = 3.68NRR540 pKa = 11.84IEE542 pKa = 3.73IMINGRR548 pKa = 11.84PVLVTWRR555 pKa = 11.84SWIIRR560 pKa = 11.84VLCIEE565 pKa = 4.39YY566 pKa = 11.04LMAIHH571 pKa = 6.66NNPQMEE577 pKa = 4.36GFLANIRR584 pKa = 11.84RR585 pKa = 11.84LHH587 pKa = 5.06MARR590 pKa = 11.84HH591 pKa = 5.08AMMEE595 pKa = 4.16KK596 pKa = 10.1SCVFLPFGSAPEE608 pKa = 4.34DD609 pKa = 3.36KK610 pKa = 10.91CNEE613 pKa = 4.24CIINNPVVAFLARR626 pKa = 11.84TWNEE630 pKa = 3.55LPNVYY635 pKa = 8.89MM636 pKa = 5.38

Molecular weight:
73.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A140H4W7|A0A140H4W7_9ORTO RNA-directed RNA polymerase catalytic subunit OS=Bourbon virus OX=1618189 GN=PB1 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 5.25GNEE5 pKa = 3.93TAKK8 pKa = 10.8GIKK11 pKa = 10.1ALIKK15 pKa = 10.2KK16 pKa = 9.93YY17 pKa = 10.43KK18 pKa = 9.47EE19 pKa = 3.98LSNEE23 pKa = 3.7PLFQEE28 pKa = 4.1KK29 pKa = 10.4RR30 pKa = 11.84IWEE33 pKa = 3.95YY34 pKa = 10.95DD35 pKa = 3.16TFKK38 pKa = 11.0RR39 pKa = 11.84YY40 pKa = 6.42TTSKK44 pKa = 10.14KK45 pKa = 10.26DD46 pKa = 3.53HH47 pKa = 6.77APHH50 pKa = 6.07TRR52 pKa = 11.84LVYY55 pKa = 9.94NVRR58 pKa = 11.84RR59 pKa = 11.84SYY61 pKa = 10.65PLQVLPTLPEE71 pKa = 4.02VYY73 pKa = 9.82KK74 pKa = 10.25GHH76 pKa = 6.88RR77 pKa = 11.84LCKK80 pKa = 9.7RR81 pKa = 11.84EE82 pKa = 3.66VDD84 pKa = 3.03IGEE87 pKa = 4.32SKK89 pKa = 10.79RR90 pKa = 11.84ILGTIYY96 pKa = 10.87CPDD99 pKa = 2.89YY100 pKa = 10.82WMSQGEE106 pKa = 4.27PTSKK110 pKa = 10.53EE111 pKa = 3.63IAEE114 pKa = 4.22SLIGPEE120 pKa = 3.73MDD122 pKa = 3.04KK123 pKa = 11.02VDD125 pKa = 5.3RR126 pKa = 11.84FFQASWGDD134 pKa = 3.53LQYY137 pKa = 11.79GHH139 pKa = 7.93LLPYY143 pKa = 9.67RR144 pKa = 11.84KK145 pKa = 8.81PVSIVPVIQEE155 pKa = 3.92VTPGSIAPTLEE166 pKa = 4.29QVFCPQFSTIDD177 pKa = 3.14AAGRR181 pKa = 11.84SNRR184 pKa = 11.84EE185 pKa = 3.75AVEE188 pKa = 4.45KK189 pKa = 9.01ITKK192 pKa = 10.14KK193 pKa = 10.02IRR195 pKa = 11.84PLLSLKK201 pKa = 8.83STPALIHH208 pKa = 6.42LARR211 pKa = 11.84GLITQRR217 pKa = 11.84KK218 pKa = 7.2KK219 pKa = 9.68WVPVTIDD226 pKa = 3.7TNPSTAEE233 pKa = 3.84LSHH236 pKa = 6.65FLYY239 pKa = 10.57SSYY242 pKa = 11.14HH243 pKa = 5.89HH244 pKa = 6.83LPVSKK249 pKa = 10.65VHH251 pKa = 6.25TIEE254 pKa = 3.84TASIEE259 pKa = 4.21RR260 pKa = 11.84LCGEE264 pKa = 4.32IVQWALKK271 pKa = 8.13TWEE274 pKa = 4.29PKK276 pKa = 10.29KK277 pKa = 10.79KK278 pKa = 10.33LLEE281 pKa = 4.28LLEE284 pKa = 4.88KK285 pKa = 9.76ITVKK289 pKa = 9.69GTSLSSILEE298 pKa = 4.08SLDD301 pKa = 3.13SDD303 pKa = 3.61KK304 pKa = 11.27QYY306 pKa = 11.49TSICKK311 pKa = 9.31VALGIPIHH319 pKa = 6.36LVNQIRR325 pKa = 11.84EE326 pKa = 4.06TRR328 pKa = 11.84FVIMNRR334 pKa = 11.84EE335 pKa = 3.87AEE337 pKa = 4.46RR338 pKa = 11.84INVEE342 pKa = 4.24CIKK345 pKa = 10.81KK346 pKa = 10.27FGNIQYY352 pKa = 9.02LHH354 pKa = 7.03KK355 pKa = 9.56YY356 pKa = 8.72QRR358 pKa = 11.84FEE360 pKa = 3.75GPYY363 pKa = 9.91KK364 pKa = 10.92VFFHH368 pKa = 6.19WRR370 pKa = 11.84HH371 pKa = 4.47VKK373 pKa = 10.65GIIIGSDD380 pKa = 3.07MKK382 pKa = 10.45ISKK385 pKa = 9.94ILLHH389 pKa = 6.89AEE391 pKa = 3.83QGDD394 pKa = 3.92FLLPLVLDD402 pKa = 3.58ILYY405 pKa = 8.23YY406 pKa = 9.57TASLEE411 pKa = 4.23PGFEE415 pKa = 3.61RR416 pKa = 11.84SYY418 pKa = 11.51DD419 pKa = 3.45QFVEE423 pKa = 4.18KK424 pKa = 10.72KK425 pKa = 10.04NLFSHH430 pKa = 7.11FFAHH434 pKa = 6.23HH435 pKa = 6.07TSNPLEE441 pKa = 4.36VYY443 pKa = 9.92RR444 pKa = 11.84VLGVGPTGALANSFHH459 pKa = 6.35WKK461 pKa = 9.3IVSEE465 pKa = 4.3GFLSKK470 pKa = 10.0IGEE473 pKa = 4.54MEE475 pKa = 4.28TSVQPYY481 pKa = 8.31SAYY484 pKa = 10.54AATHH488 pKa = 5.27QLSDD492 pKa = 3.33QLEE495 pKa = 4.15VFRR498 pKa = 11.84ISKK501 pKa = 9.96HH502 pKa = 5.55SKK504 pKa = 10.03HH505 pKa = 6.57KK506 pKa = 10.93LIDD509 pKa = 4.2PNEE512 pKa = 3.97LLVPKK517 pKa = 10.03TPLPLGLDD525 pKa = 3.4ATSTEE530 pKa = 4.24LVDD533 pKa = 5.34VITNPLVKK541 pKa = 10.37AKK543 pKa = 9.68TMWTKK548 pKa = 11.17LITDD552 pKa = 3.94PMRR555 pKa = 11.84CKK557 pKa = 10.2TFVSNAITGNPDD569 pKa = 3.04PFMKK573 pKa = 10.02TVQQIMPPRR582 pKa = 11.84SRR584 pKa = 11.84VANKK588 pKa = 9.63RR589 pKa = 11.84RR590 pKa = 11.84LEE592 pKa = 3.96EE593 pKa = 4.35LVEE596 pKa = 5.52DD597 pKa = 4.36DD598 pKa = 4.92PNWTRR603 pKa = 11.84SPEE606 pKa = 4.04KK607 pKa = 10.14RR608 pKa = 11.84FKK610 pKa = 10.86AAFDD614 pKa = 3.74AAVLLAGKK622 pKa = 8.89PKK624 pKa = 9.98KK625 pKa = 10.18RR626 pKa = 11.84PAEE629 pKa = 4.2GPPLNKK635 pKa = 9.72VAMSCLGEE643 pKa = 4.07TLDD646 pKa = 3.93YY647 pKa = 11.44SMSGGLVVSTGHH659 pKa = 7.54KK660 pKa = 9.13IRR662 pKa = 11.84VMSQIVVDD670 pKa = 3.95NSITPVEE677 pKa = 4.22GFVYY681 pKa = 9.97TGVYY685 pKa = 7.84TEE687 pKa = 4.76KK688 pKa = 10.45PKK690 pKa = 11.25DD691 pKa = 3.61MEE693 pKa = 4.33VTTLEE698 pKa = 4.46DD699 pKa = 3.28AMKK702 pKa = 9.87MRR704 pKa = 11.84LKK706 pKa = 10.44RR707 pKa = 11.84ICLMQHH713 pKa = 3.83NRR715 pKa = 11.84YY716 pKa = 8.92YY717 pKa = 10.88VLEE720 pKa = 4.52EE721 pKa = 4.08KK722 pKa = 10.24TSLWSAIEE730 pKa = 4.12NIRR733 pKa = 11.84TTIDD737 pKa = 3.18HH738 pKa = 5.37QQSMIKK744 pKa = 10.4ASQVDD749 pKa = 3.92EE750 pKa = 4.6ALLSLGAPGPSWKK763 pKa = 10.61

Molecular weight:
86.86 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3356

270

763

559.3

63.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.274 ± 0.255

2.056 ± 0.345

4.678 ± 0.24

7.449 ± 0.498

3.903 ± 0.313

5.334 ± 0.622

2.443 ± 0.246

5.423 ± 0.5

7.509 ± 0.484

9.803 ± 0.528

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.297

4.112 ± 0.498

4.768 ± 0.557

3.725 ± 0.358

5.274 ± 0.41

7.33 ± 0.423

6.168 ± 0.268

6.943 ± 0.437

1.639 ± 0.259

3.248 ± 0.185

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski