Saccharothrix sp. ALI-22-I

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharothrix; unclassified Saccharothrix

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8733 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2Q5Y3|A0A1V2Q5Y3_9PSEU Cupin OS=Saccharothrix sp. ALI-22-I OX=1933778 GN=ALI22I_34615 PE=4 SV=1
MM1 pKa = 7.09SAEE4 pKa = 3.34IRR6 pKa = 11.84IYY8 pKa = 10.27HH9 pKa = 7.09AYY11 pKa = 10.65SPDD14 pKa = 3.04QYY16 pKa = 11.39AQVVEE21 pKa = 4.07LWEE24 pKa = 4.43RR25 pKa = 11.84LLSCHH30 pKa = 6.47RR31 pKa = 11.84VGLSEE36 pKa = 4.12DD37 pKa = 3.55RR38 pKa = 11.84YY39 pKa = 11.31GRR41 pKa = 11.84GLDD44 pKa = 3.46KK45 pKa = 10.84IEE47 pKa = 4.71PEE49 pKa = 3.65WLYY52 pKa = 11.05QLVMSDD58 pKa = 3.18GAEE61 pKa = 4.21TYY63 pKa = 9.96DD64 pKa = 3.79YY65 pKa = 10.64PAVTVTVAEE74 pKa = 4.11YY75 pKa = 10.93SDD77 pKa = 4.26YY78 pKa = 11.74NGDD81 pKa = 3.7EE82 pKa = 3.88YY83 pKa = 11.55DD84 pKa = 3.59AANVAVLEE92 pKa = 4.25DD93 pKa = 3.69QYY95 pKa = 12.19GLNTRR100 pKa = 11.84GGSHH104 pKa = 6.33GWQAAWVQLGEE115 pKa = 4.56LPVITDD121 pKa = 3.31DD122 pKa = 4.42TIDD125 pKa = 3.43VGIEE129 pKa = 3.75RR130 pKa = 11.84LKK132 pKa = 10.07TLVEE136 pKa = 4.15VVEE139 pKa = 5.23ALTQGDD145 pKa = 4.97VVCLDD150 pKa = 4.03DD151 pKa = 6.78DD152 pKa = 3.77VLEE155 pKa = 4.38DD156 pKa = 3.43HH157 pKa = 6.55RR158 pKa = 11.84QAVIEE163 pKa = 4.27DD164 pKa = 3.47TWVNYY169 pKa = 7.28YY170 pKa = 10.74ARR172 pKa = 11.84EE173 pKa = 3.9LSSALEE179 pKa = 4.44DD180 pKa = 3.48LTDD183 pKa = 3.79YY184 pKa = 11.52NSDD187 pKa = 3.73DD188 pKa = 4.33LGFSDD193 pKa = 5.85EE194 pKa = 4.9EE195 pKa = 4.06IKK197 pKa = 10.92SLYY200 pKa = 10.62FEE202 pKa = 5.4FEE204 pKa = 4.02GNDD207 pKa = 3.26WEE209 pKa = 4.47FQGATEE215 pKa = 4.08ITNNGHH221 pKa = 6.77DD222 pKa = 3.69EE223 pKa = 4.19AVEE226 pKa = 3.96HH227 pKa = 6.22VIEE230 pKa = 4.83TIRR233 pKa = 11.84DD234 pKa = 3.21AWRR237 pKa = 11.84APYY240 pKa = 10.31VDD242 pKa = 4.57PDD244 pKa = 3.74QFALPLASS252 pKa = 3.68

Molecular weight: 28.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2Q1X3|A0A1V2Q1X3_9PSEU Uncharacterized protein OS=Saccharothrix sp. ALI-22-I OX=1933778 GN=ALI22I_39225 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.38GRR42 pKa = 11.84QQLSAA47 pKa = 4.05

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8733

0

8733

2886714

31

8808

330.6

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.852 ± 0.038

0.754 ± 0.007

6.293 ± 0.027

5.445 ± 0.022

2.898 ± 0.016

9.041 ± 0.026

2.351 ± 0.014

3.192 ± 0.02

1.974 ± 0.019

10.395 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.664 ± 0.011

2.029 ± 0.02

5.88 ± 0.024

2.786 ± 0.016

8.0 ± 0.031

5.107 ± 0.02

6.233 ± 0.029

9.475 ± 0.031

1.62 ± 0.011

2.009 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski