Euonymus yellow vein virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218MK54|A0A218MK54_9VIRU TGB 2 protein OS=Euonymus yellow vein virus OX=2013968 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 4.53VLTRR6 pKa = 11.84LLNEE10 pKa = 3.71NRR12 pKa = 11.84FLRR15 pKa = 11.84TLEE18 pKa = 5.0PISKK22 pKa = 9.38PLVIHH27 pKa = 6.67ACAGAGKK34 pKa = 8.35STIIRR39 pKa = 11.84SVLNSVPGARR49 pKa = 11.84AYY51 pKa = 9.68TFGKK55 pKa = 10.24ADD57 pKa = 3.65KK58 pKa = 10.3KK59 pKa = 10.75NLSGQFIEE67 pKa = 5.52SACCHH72 pKa = 6.49PKK74 pKa = 10.35PEE76 pKa = 3.98ASFRR80 pKa = 11.84ILDD83 pKa = 4.0EE84 pKa = 4.26YY85 pKa = 11.22LVSDD89 pKa = 5.19DD90 pKa = 4.0GEE92 pKa = 4.21EE93 pKa = 4.02YY94 pKa = 10.91DD95 pKa = 6.15AVFCDD100 pKa = 3.72PLQVKK105 pKa = 8.05GTARR109 pKa = 11.84RR110 pKa = 11.84PHH112 pKa = 6.88FICTTSQRR120 pKa = 11.84FGWHH124 pKa = 5.31TADD127 pKa = 4.71LLRR130 pKa = 11.84KK131 pKa = 10.03LGIEE135 pKa = 4.13LNSSKK140 pKa = 10.84EE141 pKa = 3.95DD142 pKa = 3.41LVLIQPLFEE151 pKa = 4.66GEE153 pKa = 4.11PEE155 pKa = 4.25GVILAWEE162 pKa = 4.36PEE164 pKa = 4.29VCALLDD170 pKa = 3.51DD171 pKa = 4.8HH172 pKa = 7.16LVEE175 pKa = 4.95FKK177 pKa = 10.89KK178 pKa = 10.27PSEE181 pKa = 4.4VIGEE185 pKa = 4.31TFDD188 pKa = 3.72CVSVITEE195 pKa = 4.07SLIDD199 pKa = 3.7NVDD202 pKa = 4.0FEE204 pKa = 4.83SLYY207 pKa = 10.51VALSRR212 pKa = 11.84HH213 pKa = 5.22CEE215 pKa = 3.92KK216 pKa = 10.93LVVLSADD223 pKa = 3.5PRR225 pKa = 11.84DD226 pKa = 3.71LLLISDD232 pKa = 4.93ASHH235 pKa = 6.45SAA237 pKa = 3.05

Molecular weight: 26.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218MK55|A0A218MK55_9VIRU RNA-directed RNA polymerase OS=Euonymus yellow vein virus OX=2013968 PE=4 SV=1
MM1 pKa = 7.93PLTPPRR7 pKa = 11.84DD8 pKa = 3.53YY9 pKa = 10.86STVFVVALAAFSLVAFTFTATRR31 pKa = 11.84STLPFVGDD39 pKa = 4.12NIHH42 pKa = 6.42SLAHH46 pKa = 5.93GGCYY50 pKa = 9.27RR51 pKa = 11.84DD52 pKa = 3.7GTKK55 pKa = 10.24SIQYY59 pKa = 8.19FPPTPVGSALKK70 pKa = 10.47GGYY73 pKa = 8.55LAAAITCIVIPGVLYY88 pKa = 10.44AVHH91 pKa = 7.24RR92 pKa = 11.84SNLHH96 pKa = 6.07CKK98 pKa = 9.3PVPCPNCRR106 pKa = 11.84THH108 pKa = 6.52VASPQQ113 pKa = 3.09

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2258

93

1513

451.6

50.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.193 ± 1.267

1.949 ± 0.491

5.27 ± 0.363

7.396 ± 0.716

4.163 ± 0.138

5.447 ± 0.655

2.613 ± 0.265

5.314 ± 0.407

6.687 ± 1.456

8.725 ± 0.975

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.037 ± 0.373

3.676 ± 0.4

5.757 ± 0.423

4.03 ± 0.667

5.624 ± 0.569

6.776 ± 0.438

6.599 ± 0.592

5.802 ± 0.827

1.151 ± 0.116

2.79 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski