Klebsiella phage ST147-VIM1phi7.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J9X9|A0A482J9X9_9CAUD HNH endonuclease OS=Klebsiella phage ST147-VIM1phi7.2 OX=2555912 PE=4 SV=1
MM1 pKa = 7.55TEE3 pKa = 4.25DD4 pKa = 4.64DD5 pKa = 5.78LYY7 pKa = 11.78DD8 pKa = 4.1LLSPLAEE15 pKa = 3.92GRR17 pKa = 11.84VYY19 pKa = 10.49PYY21 pKa = 10.75VVSLGSDD28 pKa = 4.0GLPDD32 pKa = 3.5VPAPYY37 pKa = 9.74IIFSIPTDD45 pKa = 3.27VSGDD49 pKa = 3.84VFCGQAEE56 pKa = 4.32STLRR60 pKa = 11.84IQVDD64 pKa = 3.19VWAEE68 pKa = 4.06TNDD71 pKa = 3.14EE72 pKa = 3.92ARR74 pKa = 11.84ALRR77 pKa = 11.84LDD79 pKa = 3.39ALARR83 pKa = 11.84LQVLSPVEE91 pKa = 3.95VTKK94 pKa = 10.77IPGYY98 pKa = 9.2DD99 pKa = 3.31TTTHH103 pKa = 6.1LHH105 pKa = 6.34RR106 pKa = 11.84ATLEE110 pKa = 3.77ITVIAA115 pKa = 4.65

Molecular weight: 12.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J9W4|A0A482J9W4_9CAUD Nitrite transporter OS=Klebsiella phage ST147-VIM1phi7.2 OX=2555912 PE=4 SV=1
MM1 pKa = 7.4YY2 pKa = 10.05KK3 pKa = 9.03RR4 pKa = 11.84TAAPVDD10 pKa = 3.13AKK12 pKa = 9.79TRR14 pKa = 11.84KK15 pKa = 8.34STEE18 pKa = 3.78QQQLVANTKK27 pKa = 6.26TEE29 pKa = 3.72RR30 pKa = 11.84RR31 pKa = 11.84EE32 pKa = 3.93VSRR35 pKa = 11.84LAIWCHH41 pKa = 6.05NIHH44 pKa = 6.54SRR46 pKa = 4.12

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

10339

45

1129

229.8

25.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.817 ± 0.564

1.083 ± 0.142

5.678 ± 0.353

6.403 ± 0.346

3.395 ± 0.242

7.225 ± 0.379

1.722 ± 0.243

5.117 ± 0.239

5.281 ± 0.384

9.169 ± 0.443

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.153

4.014 ± 0.259

4.188 ± 0.35

4.246 ± 0.423

6.209 ± 0.406

6.751 ± 0.367

6.258 ± 0.364

6.442 ± 0.263

1.567 ± 0.147

2.94 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski