Peptostreptococcaceae bacterium oral taxon 113 str. W5053

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; unclassified Peptostreptococcaceae; Peptostreptococcaceae bacterium oral taxon 113

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2JEV3|U2JEV3_9FIRM ABC transporter ATP-binding protein OS=Peptostreptococcaceae bacterium oral taxon 113 str. W5053 OX=1321784 GN=HMPREF1987_00382 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.09EE3 pKa = 4.08KK4 pKa = 10.49IMSEE8 pKa = 4.14KK9 pKa = 10.39NLHH12 pKa = 6.19NDD14 pKa = 3.11EE15 pKa = 5.09CGCGCNHH22 pKa = 7.26DD23 pKa = 4.52LDD25 pKa = 4.2EE26 pKa = 4.72EE27 pKa = 4.62FYY29 pKa = 11.01EE30 pKa = 5.2DD31 pKa = 3.83EE32 pKa = 4.6LEE34 pKa = 4.41IMTLEE39 pKa = 4.13TEE41 pKa = 3.92EE42 pKa = 4.71GEE44 pKa = 4.28RR45 pKa = 11.84EE46 pKa = 3.89VGIIGVFGIEE56 pKa = 3.96EE57 pKa = 3.86QEE59 pKa = 4.26YY60 pKa = 10.22IALLSLEE67 pKa = 4.35TEE69 pKa = 3.99EE70 pKa = 4.38VMVYY74 pKa = 9.74RR75 pKa = 11.84YY76 pKa = 9.32YY77 pKa = 10.8EE78 pKa = 4.2KK79 pKa = 10.79EE80 pKa = 3.91DD81 pKa = 3.76GEE83 pKa = 4.52FEE85 pKa = 4.44LGSIEE90 pKa = 4.4SDD92 pKa = 3.12EE93 pKa = 4.28EE94 pKa = 4.63YY95 pKa = 11.36EE96 pKa = 4.12MVEE99 pKa = 4.13EE100 pKa = 4.37VFNTLYY106 pKa = 10.96VEE108 pKa = 4.18EE109 pKa = 5.43DD110 pKa = 3.44EE111 pKa = 5.61SEE113 pKa = 4.17EE114 pKa = 4.03

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2LDY5|U2LDY5_9FIRM Uncharacterized protein OS=Peptostreptococcaceae bacterium oral taxon 113 str. W5053 OX=1321784 GN=HMPREF1987_00007 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSIIKK11 pKa = 10.33RR12 pKa = 11.84KK13 pKa = 8.34RR14 pKa = 11.84NHH16 pKa = 5.66GFRR19 pKa = 11.84EE20 pKa = 3.84RR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.54NGRR28 pKa = 11.84LVLKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.33GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2325

0

2325

625010

39

2162

268.8

30.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.08 ± 0.055

1.123 ± 0.021

5.034 ± 0.046

7.871 ± 0.074

4.862 ± 0.05

6.735 ± 0.049

1.813 ± 0.022

8.456 ± 0.062

8.338 ± 0.058

9.804 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.017 ± 0.027

4.745 ± 0.038

3.056 ± 0.031

3.111 ± 0.031

4.324 ± 0.041

5.885 ± 0.041

4.696 ± 0.04

6.205 ± 0.039

0.87 ± 0.018

3.974 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski