Chaetoceros protobacilladnavirus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Baphyvirales; Bacilladnaviridae; Protobacilladnavirus

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3WH29|E3WH29_9VIRU Putative replication-associated protein OS=Chaetoceros protobacilladnavirus 4 OX=2170143 PE=4 SV=1
MM1 pKa = 7.61HH2 pKa = 7.31FRR4 pKa = 11.84NMSNNSFDD12 pKa = 3.76IQEE15 pKa = 5.05LICDD19 pKa = 4.48DD20 pKa = 4.47ASIDD24 pKa = 3.76CSISINTTPSVDD36 pKa = 3.97FLEE39 pKa = 4.87TEE41 pKa = 4.31LKK43 pKa = 10.78EE44 pKa = 4.22PTNNTLISRR53 pKa = 11.84AILTYY58 pKa = 9.92FPPTSDD64 pKa = 3.07AKK66 pKa = 10.21YY67 pKa = 10.46LEE69 pKa = 4.5PEE71 pKa = 4.08FMLGQDD77 pKa = 4.03IRR79 pKa = 11.84RR80 pKa = 11.84LSNWWSQYY88 pKa = 7.88EE89 pKa = 4.03KK90 pKa = 10.44CPEE93 pKa = 3.95SGAVHH98 pKa = 5.45IHH100 pKa = 6.76AYY102 pKa = 10.68AEE104 pKa = 4.76FKK106 pKa = 10.86RR107 pKa = 11.84EE108 pKa = 3.69LRR110 pKa = 11.84PRR112 pKa = 11.84FNLLDD117 pKa = 3.64EE118 pKa = 5.66LIRR121 pKa = 11.84SHH123 pKa = 6.73VDD125 pKa = 2.6CWNCRR130 pKa = 11.84TARR133 pKa = 11.84KK134 pKa = 9.69SSEE137 pKa = 4.02SQRR140 pKa = 11.84QGAINYY146 pKa = 5.81VHH148 pKa = 7.06KK149 pKa = 10.95FEE151 pKa = 4.29TRR153 pKa = 11.84LDD155 pKa = 3.63GFQPFTWPKK164 pKa = 9.94NEE166 pKa = 4.25SNAKK170 pKa = 9.6FDD172 pKa = 3.92PSFVRR177 pKa = 11.84TNSKK181 pKa = 8.91QNKK184 pKa = 8.58RR185 pKa = 11.84KK186 pKa = 10.12ARR188 pKa = 11.84DD189 pKa = 3.44DD190 pKa = 4.49AIEE193 pKa = 3.9EE194 pKa = 3.69QRR196 pKa = 11.84QYY198 pKa = 11.01IEE200 pKa = 4.12SKK202 pKa = 9.72PRR204 pKa = 11.84IWTWDD209 pKa = 3.36QILHH213 pKa = 5.84EE214 pKa = 4.65NEE216 pKa = 3.78ASKK219 pKa = 11.12KK220 pKa = 10.7LLVTCSWGKK229 pKa = 9.75KK230 pKa = 6.24YY231 pKa = 10.62HH232 pKa = 6.38EE233 pKa = 4.89GRR235 pKa = 11.84HH236 pKa = 5.65AEE238 pKa = 4.14DD239 pKa = 3.29KK240 pKa = 11.0RR241 pKa = 11.84RR242 pKa = 11.84DD243 pKa = 3.52VTDD246 pKa = 3.59VIIYY250 pKa = 9.63YY251 pKa = 10.43GAGGTGKK258 pKa = 7.43TTLCMEE264 pKa = 4.41YY265 pKa = 10.24NQTSSLEE272 pKa = 4.11PRR274 pKa = 11.84VEE276 pKa = 3.56RR277 pKa = 11.84YY278 pKa = 8.52YY279 pKa = 10.96RR280 pKa = 11.84RR281 pKa = 11.84NPDD284 pKa = 4.5DD285 pKa = 4.03GNFWGGGRR293 pKa = 11.84TAYY296 pKa = 9.76KK297 pKa = 8.82GQRR300 pKa = 11.84VIHH303 pKa = 6.06FEE305 pKa = 4.02EE306 pKa = 5.18FSGQEE311 pKa = 3.48PFSRR315 pKa = 11.84LKK317 pKa = 10.11EE318 pKa = 3.9VCDD321 pKa = 3.38IGKK324 pKa = 9.09PGPSVNIKK332 pKa = 10.38GAGVDD337 pKa = 4.25LNHH340 pKa = 6.34HH341 pKa = 5.45TVMFSSNVHH350 pKa = 5.23PAGWYY355 pKa = 8.66RR356 pKa = 11.84NLWRR360 pKa = 11.84DD361 pKa = 3.71DD362 pKa = 3.67PKK364 pKa = 10.89QFHH367 pKa = 6.28PFWRR371 pKa = 11.84RR372 pKa = 11.84VTKK375 pKa = 10.38VVFFPSHH382 pKa = 6.56RR383 pKa = 11.84PDD385 pKa = 3.01GSMNIPDD392 pKa = 4.62EE393 pKa = 5.01DD394 pKa = 4.42NPPHH398 pKa = 6.78MIDD401 pKa = 4.98QTDD404 pKa = 2.86EE405 pKa = 3.8WKK407 pKa = 10.81ALNGSYY413 pKa = 8.81EE414 pKa = 3.97ACMEE418 pKa = 4.51HH419 pKa = 8.19ADD421 pKa = 3.62EE422 pKa = 4.59HH423 pKa = 6.04WPVRR427 pKa = 11.84EE428 pKa = 3.8VSVDD432 pKa = 3.48GFVKK436 pKa = 10.54SFNHH440 pKa = 5.54SHH442 pKa = 4.64QTSAA446 pKa = 4.16

Molecular weight: 52.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3WH28|E3WH28_9VIRU Uncharacterized protein OS=Chaetoceros protobacilladnavirus 4 OX=2170143 PE=4 SV=1
MM1 pKa = 6.8VQHH4 pKa = 7.2DD5 pKa = 3.98VQNIGRR11 pKa = 11.84KK12 pKa = 8.72RR13 pKa = 11.84DD14 pKa = 3.59FRR16 pKa = 11.84TVTPEE21 pKa = 3.93APLEE25 pKa = 4.21MPSGQRR31 pKa = 11.84LHH33 pKa = 5.5YY34 pKa = 9.69TEE36 pKa = 5.28DD37 pKa = 3.59LAVIGPEE44 pKa = 4.16PEE46 pKa = 3.77RR47 pKa = 11.84APYY50 pKa = 10.27DD51 pKa = 3.46IVPFAGDD58 pKa = 3.41EE59 pKa = 4.29LKK61 pKa = 11.03EE62 pKa = 3.92QMTLIRR68 pKa = 11.84NNGMTEE74 pKa = 3.86QQAISYY80 pKa = 7.28FTDD83 pKa = 3.62YY84 pKa = 11.44AEE86 pKa = 4.63KK87 pKa = 10.33SHH89 pKa = 7.82LGEE92 pKa = 4.66FSAAAYY98 pKa = 7.6SAPSGVAIRR107 pKa = 11.84VNPHH111 pKa = 5.88TGLKK115 pKa = 9.46EE116 pKa = 3.69AFIAGTRR123 pKa = 11.84GPLDD127 pKa = 3.04WGRR130 pKa = 11.84NIIEE134 pKa = 4.41GADD137 pKa = 3.85SIVSAGLHH145 pKa = 5.26KK146 pKa = 10.04VTNIMPKK153 pKa = 10.48GEE155 pKa = 4.3LRR157 pKa = 11.84NKK159 pKa = 10.4AEE161 pKa = 3.75AAANVLEE168 pKa = 4.33NVSVPGRR175 pKa = 11.84ISKK178 pKa = 10.19ILTRR182 pKa = 11.84SEE184 pKa = 4.07GEE186 pKa = 3.87EE187 pKa = 3.58LAEE190 pKa = 4.16TFRR193 pKa = 11.84EE194 pKa = 4.02EE195 pKa = 4.38GVQVIYY201 pKa = 10.33GHH203 pKa = 6.03SRR205 pKa = 11.84AGAILQYY212 pKa = 10.46FPEE215 pKa = 4.49EE216 pKa = 4.19EE217 pKa = 4.22FTTIALDD224 pKa = 3.51GANIVGHH231 pKa = 7.1DD232 pKa = 4.29PMSAPNFSSDD242 pKa = 4.55DD243 pKa = 3.34IFSRR247 pKa = 11.84TIGIGSRR254 pKa = 11.84KK255 pKa = 9.31RR256 pKa = 11.84VEE258 pKa = 3.73LPGRR262 pKa = 11.84SFHH265 pKa = 7.05KK266 pKa = 10.49AWEE269 pKa = 4.41TKK271 pKa = 9.87AKK273 pKa = 9.72KK274 pKa = 10.38AKK276 pKa = 9.83ADD278 pKa = 3.47KK279 pKa = 10.48KK280 pKa = 11.14AEE282 pKa = 4.0DD283 pKa = 3.61RR284 pKa = 11.84KK285 pKa = 10.37KK286 pKa = 11.09SKK288 pKa = 10.79LGVPLAEE295 pKa = 4.46LKK297 pKa = 10.88KK298 pKa = 10.55EE299 pKa = 4.26FYY301 pKa = 10.2EE302 pKa = 4.3QADD305 pKa = 3.58KK306 pKa = 10.42ATRR309 pKa = 11.84RR310 pKa = 11.84QHH312 pKa = 4.87MKK314 pKa = 9.94LVEE317 pKa = 3.94RR318 pKa = 11.84AKK320 pKa = 10.27TKK322 pKa = 10.03IGKK325 pKa = 9.31KK326 pKa = 9.55FAANLAKK333 pKa = 10.72YY334 pKa = 9.22IAKK337 pKa = 10.12QSAEE341 pKa = 4.22TAIKK345 pKa = 10.03ALPGGSYY352 pKa = 10.7LPIGQASAPVAAYY365 pKa = 8.32PPLPRR370 pKa = 11.84LVEE373 pKa = 4.27GDD375 pKa = 3.74LRR377 pKa = 11.84SGTVVPYY384 pKa = 9.19TKK386 pKa = 10.32KK387 pKa = 10.17RR388 pKa = 11.84NKK390 pKa = 10.46KK391 pKa = 10.09KK392 pKa = 8.14MTSARR397 pKa = 11.84KK398 pKa = 9.35SKK400 pKa = 9.84YY401 pKa = 10.17ARR403 pKa = 11.84LNDD406 pKa = 4.07EE407 pKa = 4.88LPHH410 pKa = 6.39MGDD413 pKa = 2.92IDD415 pKa = 3.6

Molecular weight: 45.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1233

372

446

411.0

46.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.948 ± 1.494

1.217 ± 0.451

5.758 ± 0.477

6.569 ± 1.009

4.055 ± 0.48

6.407 ± 0.517

3.244 ± 0.441

5.353 ± 0.246

7.218 ± 0.685

6.002 ± 0.456

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.109 ± 0.143

5.921 ± 1.045

5.272 ± 0.408

3.731 ± 0.376

6.569 ± 0.393

7.218 ± 0.424

5.353 ± 0.184

5.434 ± 0.275

1.541 ± 0.592

3.082 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski