Faecalibacterium prausnitzii (strain DSM 17677 / JCM 31915 / A2-165)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Faecalibacterium; Faecalibacterium prausnitzii

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7H466|C7H466_FAEPA Uncharacterized protein OS=Faecalibacterium prausnitzii (strain DSM 17677 / JCM 31915 / A2-165) OX=411483 GN=FAEPRAA2165_01079 PE=4 SV=1
MM1 pKa = 7.67EE2 pKa = 5.76YY3 pKa = 10.03IPKK6 pKa = 9.84VLQAVAGEE14 pKa = 4.48DD15 pKa = 3.53FTIYY19 pKa = 10.53LYY21 pKa = 10.89FSDD24 pKa = 4.51GSVRR28 pKa = 11.84LYY30 pKa = 10.36DD31 pKa = 4.77AKK33 pKa = 10.83PLLQLGGVFEE43 pKa = 4.56PMRR46 pKa = 11.84DD47 pKa = 3.11PDD49 pKa = 3.78YY50 pKa = 11.18FRR52 pKa = 11.84DD53 pKa = 3.67RR54 pKa = 11.84LTVLNDD60 pKa = 3.11TAAWDD65 pKa = 3.66VSGDD69 pKa = 3.82MDD71 pKa = 4.26PCNCIDD77 pKa = 4.68LDD79 pKa = 4.61PIEE82 pKa = 5.64LYY84 pKa = 7.55EE85 pKa = 4.16TCPVVVDD92 pKa = 4.21PLEE95 pKa = 4.34KK96 pKa = 10.68ASS98 pKa = 3.55

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7H1Y3|C7H1Y3_FAEPA Uncharacterized protein OS=Faecalibacterium prausnitzii (strain DSM 17677 / JCM 31915 / A2-165) OX=411483 GN=FAEPRAA2165_00273 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.43KK9 pKa = 7.51RR10 pKa = 11.84HH11 pKa = 5.49RR12 pKa = 11.84KK13 pKa = 7.53EE14 pKa = 3.39VHH16 pKa = 5.73GFLTRR21 pKa = 11.84MSTKK25 pKa = 10.35NGRR28 pKa = 11.84KK29 pKa = 9.33VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.97SLTVV44 pKa = 3.12

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3472

0

3472

930013

37

2454

267.9

29.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.76 ± 0.059

1.746 ± 0.019

5.379 ± 0.035

6.748 ± 0.043

3.938 ± 0.034

7.374 ± 0.046

1.901 ± 0.02

5.547 ± 0.039

5.466 ± 0.04

9.812 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.024

3.683 ± 0.027

4.156 ± 0.029

3.721 ± 0.029

5.358 ± 0.041

5.451 ± 0.033

5.685 ± 0.031

6.851 ± 0.039

1.052 ± 0.015

3.561 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski